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Vol. 14, Issue 1, 129-141, January 2003
§ and
*Department of Biology, University of Rochester, Rochester,
New York 14627; Nucleus-vacuole (NV) junctions in Saccharomyces
cerevisiae are formed through specific interactions between
Vac8p on the vacuole membrane and Nvj1p in the nuclear envelope.
Herein, we report that NV junctions in yeast promote
piecemeal microautophagy of the
nucleus (PMN). During PMN, teardrop-like blebs are
pinched from the nucleus, released into the vacuole lumen, and degraded by soluble hydrolases. PMN occurs in rapidly dividing cells but is
induced to higher levels by carbon and nitrogen starvation and is under
the control of the Tor kinase nutrient-sensing pathway. Confocal and
biochemical assays demonstrate that Nvj1p is degraded in a
PMN-dependent manner. PMN occurs normally in apg7-Boulder Laboratory for
Three-Dimensional Fine Structure, University of Colorado, Boulder,
Colorado 80309-0347; and §Department of Molecular,
Cellular, and Developmental Biology, University of Colorado, Boulder,
Colorado 80309-0347
cells and is, therefore, not dependent on macroautophagy. Transmission electron microscopy reveals that portions of the granular nucleolus are
often sequestered into PMN structures. These results introduce a novel
mode of selective microautophagy that targets nonessential components
of the yeast nucleus for degradation and recycling in the vacuole.
These authors contributed equally to this study.
Corresponding author. E-mail address:
dasg{at}mail.rochester.edu.
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