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Vol. 14, Issue 1, 190-200, January 2003
and
§
*Department of Developmental Biology and
The multisubunit conserved oligomeric Golgi (COG) complex
has been shown previously to be involved in Golgi function in yeast and
mammalian tissue culture cells. Despite this broad conservation, several subunits, including Cog5, were not essential for growth and
showed only mild effects on secretion when mutated in yeast, raising
questions about what functions these COG complex subunits play in the
life of the cell. Here, we show that function of the gene four
way stop (fws), which encodes the
Drosophila Cog5 homologue, is necessary for dramatic
changes in cellular and subcellular morphology during spermatogenesis.
Loss-of-function mutations in fws caused failure of
cleavage furrow ingression in dividing spermatocytes and failure of
cell elongation in differentiating spermatids and disrupted the
formation and/or stability of the Golgi-based spermatid acroblast.
Consistent with the lack of a growth defect in yeast lacking Cog5,
animals lacking fws function were viable, although males
were sterile. Fws protein localized to Golgi structures throughout
spermatogenesis. We propose that Fws may directly or indirectly
facilitate efficient vesicle traffic through the Golgi to support rapid
and extensive increases in cell surface area during spermatocyte
cytokinesis and polarized elongation of differentiating spermatids. Our
study suggests that Drosophila spermatogenesis can be an
effective sensitized genetic system to uncover in vivo functions for
proteins involved in Golgi architecture and/or vesicle transport.
Department of Genetics, Stanford University
School of Medicine, Stanford, California 94305-5329, and
Istituto Pasteur Fondazionie Cenci Bolognetti and
Centro di Genetica Evoluzionistica del CNR, Dipartimento di Genetica e
Biologia Molecolare, Universitá La Sapienza, Rome, Italy
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