![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Vol. 14, Issue 1, 274-287, January 2003
ois-Michel
Boisvert,*
and
*Terry Fox Molecular Oncology Group and the Bloomfield
Center for Research on Aging, Lady Davis Institute for Medical
Research, Sir Mortimer B. Davis Jewish General Hospital, and
Departments of Oncology, Medicine, Microbiology, and Immunology, McGill
University, Montréal, Québec, H3T 1E2 Canada; and
RNA binding proteins often contain multiple arginine glycine
repeats, a sequence that is frequently methylated by protein arginine
methyltransferases. The role of this posttranslational modification in
the life cycle of RNA binding proteins is not well understood. Herein,
we report that Sam68, a heteronuclear ribonucleoprotein K
homology domain containing RNA binding protein, associates with and is
methylated in vivo by the protein arginine N-methyltransferase 1 (PRMT1). Sam68 contains
asymmetrical dimethylarginines near its proline motif P3 as assessed by
using a novel asymmetrical dimethylarginine-specific antibody and mass
spectrometry. Deletion of the methylation sites and the use of
methylase inhibitors resulted in Sam68 accumulation in the cytoplasm.
Sam68 was also detected in the cytoplasm of PRMT1-deficient embryonic
stem cells. Although the cellular function of Sam68 is unknown, it has
been shown to export unspliced human immunodeficiency virus RNAs. Cells
treated with methylase inhibitors prevented the ability of Sam68 to
export unspliced human immunodeficiency virus RNAs. Other K homology domain RNA binding proteins, including SLM-1, SLM-2, QKI-5, GRP33, and
heteronuclear ribonucleoprotein K were also methylated in vivo. These
findings demonstrate that RNA binding proteins are in vivo substrates
for PRMT1, and their methylation is essential for their proper
localization and function.
M.D. Anderson Cancer Center, Department of
Carcinogenesis, University of Texas, Smithville, Texas 78957
These authors contributed equally to this work.
§
Corresponding author. E-mail address:
stephane.richard{at}mcgill.ca.
This article has been cited by other articles:
|
|
 |
|
  K. Fronz, S. Otto, K. Kolbel, U. Kuhn, H. Friedrich, A. Schierhorn, A. G. Beck-Sickinger, A. Ostareck-Lederer, and E. Wahle Promiscuous Modification of the Nuclear Poly(A)-binding Protein by Multiple Protein-arginine Methyltransferases Does Not Affect the Aggregation Behavior J. Biol. Chem., July 18, 2008; 283(29): 20408 - 20420. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. M. Lakowski and A. Frankel A Kinetic Study of Human Protein Arginine N-Methyltransferase 6 Reveals a Distributive Mechanism J. Biol. Chem., April 11, 2008; 283(15): 10015 - 10025. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Tadesse, J. Deschenes-Furry, S. Boisvenue, and J. Cote KH-type splicing regulatory protein interacts with survival motor neuron protein and is misregulated in spinal muscular atrophy Hum. Mol. Genet., February 14, 2008; 17(4): 506 - 524. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Goulet, G. Gauvin, S. Boisvenue, and J. Cote Alternative Splicing Yields Protein Arginine Methyltransferase 1 Isoforms with Distinct Activity, Substrate Specificity, and Subcellular Localization J. Biol. Chem., November 9, 2007; 282(45): 33009 - 33021. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. E. McBride, C. Zurita-Lopez, A. Regis, E. Blum, A. Conboy, S. Elf, and S. Clarke Protein Arginine Methylation in Candida albicans: Role in Nuclear Transport Eukaryot. Cell, July 1, 2007; 6(7): 1119 - 1129. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Bachand Protein Arginine Methyltransferases: from Unicellular Eukaryotes to Humans Eukaryot. Cell, June 1, 2007; 6(6): 889 - 898. [Full Text] [PDF]
|
|
|
|
 |
|
 B. Xie, C. F. Invernizzi, S. Richard, and M. A. Wainberg Arginine Methylation of the Human Immunodeficiency Virus Type 1 Tat Protein by PRMT6 Negatively Affects Tat Interactions with both Cyclin T1 and the Tat Transactivation Region J. Virol., April 15, 2007; 81(8): 4226 - 4234. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. P. Paronetto, T. Achsel, A. Massiello, C. E. Chalfant, and C. Sette The RNA-binding protein Sam68 modulates the alternative splicing of Bcl-x J. Cell Biol., March 26, 2007; 176(7): 929 - 939. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Perreault, C. Lemieux, and F. Bachand Regulation of the Nuclear Poly(A)-binding Protein by Arginine Methylation in Fission Yeast J. Biol. Chem., March 9, 2007; 282(10): 7552 - 7562. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. C. Goulah and L. K. Read Differential Effects of Arginine Methylation on RBP16 mRNA Binding, Guide RNA (gRNA) Binding, and gRNA-containing Ribonucleoprotein Complex (gRNP) Formation J. Biol. Chem., March 9, 2007; 282(10): 7181 - 7190. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Robin-Lespinasse, S. Sentis, C. Kolytcheff, M.-C. Rostan, L. Corbo, and M. Le Romancer hCAF1, a new regulator of PRMT1-dependent arginine methylation J. Cell Sci., February 15, 2007; 120(4): 638 - 647. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. El-Andaloussi, T. Valovka, M. Toueille, P. O. Hassa, P. Gehrig, M. Covic, U. Hubscher, and M. O. Hottiger Methylation of DNA polymerase {beta} by protein arginine methyltransferase 1 regulates its binding to proliferating cell nuclear antigen FASEB J, January 1, 2007; 21(1): 26 - 34. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. P. Tan and S. Nakielny Control of the DNA Methylation System Component MBD2 by Protein Arginine Methylation. Mol. Cell. Biol., October 1, 2006; 26(19): 7224 - 7235. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. O. Yildirim, P. Bulau, D. Zakrzewicz, K. E. Kitowska, N. Weissmann, F. Grimminger, R. E. Morty, and O. Eickelberg Increased Protein Arginine Methylation in Chronic Hypoxia: Role of Protein Arginine Methyltransferases Am. J. Respir. Cell Mol. Biol., October 1, 2006; 35(4): 436 - 443. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. C. Goulah, M. Pelletier, and L. K. Read Arginine methylation regulates mitochondrial gene expression in Trypanosoma brucei through multiple effector proteins RNA, August 1, 2006; 12(8): 1545 - 1555. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Shire, P. Kapoor, K. Jiang, M. N. T. Hing, N. Sivachandran, T. Nguyen, and L. Frappier Regulation of the EBNA1 Epstein-Barr Virus Protein by Serine Phosphorylation and Arginine Methylation. J. Virol., June 1, 2006; 80(11): 5261 - 5272. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Dolzhanskaya, G. Merz, J. M. Aletta, and R. B. Denman Methylation regulates the intracellular protein-protein and protein-RNA interactions of FMRP. J. Cell Sci., May 1, 2006; 119(Pt 9): 1933 - 1946. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Ostareck-Lederer, D. H. Ostareck, K. P. Rucknagel, A. Schierhorn, B. Moritz, S. Huttelmaier, N. Flach, L. Handoko, and E. Wahle Asymmetric Arginine Dimethylation of Heterogeneous Nuclear Ribonucleoprotein K by Protein-arginine Methyltransferase 1 Inhibits Its Interaction with c-Src J. Biol. Chem., April 21, 2006; 281(16): 11115 - 11125. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Fujiwara, Y. Mori, D. L. Chu, Y. Koyama, S. Miyata, H. Tanaka, K. Yachi, T. Kubo, H. Yoshikawa, and M. Tohyama CARM1 Regulates Proliferation of PC12 Cells by Methylating HuD. Mol. Cell. Biol., March 1, 2006; 26(6): 2273 - 2285. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. E. Lukong, D. Larocque, A. L. Tyner, and S. Richard Tyrosine Phosphorylation of Sam68 by Breast Tumor Kinase Regulates Intranuclear Localization and Cell Cycle Progression J. Biol. Chem., November 18, 2005; 280(46): 38639 - 38647. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Herrmann, J. Lee, M. T. Bedford, and F. O. Fackelmayer Dynamics of Human Protein Arginine Methyltransferase 1(PRMT1) in Vivo J. Biol. Chem., November 11, 2005; 280(45): 38005 - 38010. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Cote and S. Richard Tudor Domains Bind Symmetrical Dimethylated Arginines J. Biol. Chem., August 5, 2005; 280(31): 28476 - 28483. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Blanchet, A. Cardona, F. A. Letimier, M. S. Hershfield, and O. Acuto CD28 costimulatory signal induces protein arginine methylation in T cells J. Exp. Med., August 1, 2005; 202(3): 371 - 377. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Y. Lee, C. Teyssier, B. D. Strahl, and M. R. Stallcup Role of Protein Methylation in Regulation of Transcription Endocr. Rev., April 1, 2005; 26(2): 147 - 170. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F.-M. Boisvert, U. Dery, J.-Y. Masson, and S. Richard Arginine methylation of MRE11 by PRMT1 is required for DNA damage checkpoint control Genes & Dev., March 15, 2005; 19(6): 671 - 676. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F.-M. Boisvert, C. A. Chenard, and S. Richard Protein Interfaces in Signaling Regulated by Arginine Methylation Sci. Signal., February 15, 2005; 2005(271): re2 - re2. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Haegebarth, D. Heap, W. Bie, J. J. Derry, S. Richard, and A. L. Tyner The Nuclear Tyrosine Kinase BRK/Sik Phosphorylates and Inhibits the RNA-binding Activities of the Sam68-like Mammalian Proteins SLM-1 and SLM-2 J. Biol. Chem., December 24, 2004; 279(52): 54398 - 54404. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Herrmann, M. Bossert, A. Schwander, E. Akgun, and F. O. Fackelmayer Arginine Methylation of Scaffold Attachment Factor A by Heterogeneous Nuclear Ribonucleoprotein Particle-associated PRMT1 J. Biol. Chem., November 19, 2004; 279(47): 48774 - 48779. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D.-H. Chen, K.-T. Wu, C.-J. Hung, M. Hsieh, and C. Li Effects of Adenosine Dialdehyde Treatment on In Vitro and In Vivo Stable Protein Methylation in HeLa Cells J. Biochem., September 1, 2004; 136(3): 371 - 376. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. P. Venables, C. Dalgliesh, M. P. Paronetto, L. Skitt, J. K. Thornton, P. T. Saunders, C. Sette, K. T. Jones, and D. J. Elliott SIAH1 targets the alternative splicing factor T-STAR for degradation by the proteasome Hum. Mol. Genet., July 15, 2004; 13(14): 1525 - 1534. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Kim, J. Lee, N. Yadav, Q. Wu, C. Carter, S. Richard, E. Richie, and M. T. Bedford Loss of CARM1 Results in Hypomethylation of Thymocyte Cyclic AMP-regulated Phosphoprotein and Deregulated Early T Cell Development J. Biol. Chem., June 11, 2004; 279(24): 25339 - 25344. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Cheng, N. Yadav, R. W. King, M. S. Swanson, E. J. Weinstein, and M. T. Bedford Small Molecule Regulators of Protein Arginine Methyltransferases J. Biol. Chem., June 4, 2004; 279(23): 23892 - 23899. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Liang, Y. Lu, M. Wilkes, T. A. Neubert, and M. D. Resh The N-terminal SH4 Region of the Src Family Kinase Fyn Is Modified by Methylation and Heterogeneous Fatty Acylation: ROLE IN MEMBRANE TARGETING, CELL ADHESION, AND SPREADING J. Biol. Chem., February 27, 2004; 279(9): 8133 - 8139. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F.-M. Boisvert, J. Cote, M.-C. Boulanger, and S. Richard A Proteomic Analysis of Arginine-methylated Protein Complexes Mol. Cell. Proteomics, December 1, 2003; 2(12): 1319 - 1330. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Kuhn, A. Nemeth, S. Meyer, and E. Wahle The RNA Binding Domains of the Nuclear poly(A)-binding Protein J. Biol. Chem., May 2, 2003; 278(19): 16916 - 16925. [Abstract] [Full Text] [PDF]
|
|
