![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Vol. 14, Issue 1, 54-66, January 2003
4 Integrin-mediated
Leukocyte Adhesion by Transforming Growth Factor-
1
and
Department of Immunology, Centro de Investigaciones
Biológicas, Madrid, 28006 Spain
The 
4 integrins (4
1 and 47) are cell surface
heterodimers expressed mostly on leukocytes that mediate cell-cell and
cell-extracellular matrix adhesion. A characteristic feature of 4
integrins is that their adhesive activity can be subjected to
rapid modulation during the process of cell migration. Herein, we show
that transforming growth factor-1 (TGF-1) rapidly (0.5-5 min)
and transiently up-regulated 4 integrin-dependent adhesion
of different human leukocyte cell lines and human peripheral blood
lymphocytes (PBLs) to their ligands vascular cell adhesion molecule-1
(VCAM-1) and connecting segment-1/fibronectin. In addition,
TGF-1 enhanced the 4 integrin-mediated adhesion of PBLs
to tumor necrosis factor--treated human umbilical vein endothelial
cells, indicating the stimulation of 41/VCAM-1 interaction.
Although TGF-1 rapidly activated the small GTPase RhoA and the p38
mitogen-activated protein kinase, enhanced adhesion did not require
activation of both signaling molecules. Instead, polymerization of
actin cytoskeleton triggered by TGF-1 was necessary for 4
integrin-dependent up-regulated adhesion, and elevation of
intracellular cAMP opposed this up-regulation. Moreover, TGF-1
further increased cell adhesion mediated by 4 integrins in
response to the chemokine stromal cell-derived factor-1. These data
suggest that TGF-1 can potentially contribute to cell migration by
dynamically regulating cell adhesion mediated by 4 integrins.
Present address: Department of Hematology, Mount
Sinai School of Medicine, One Gustave Lane, Levy Place, New York, NY 10029.
Corresponding author. E-mail address:
joaquint{at}cib.csic.es.
This article has been cited by other articles:
|
|
 |
|
  M. Parmo-Cabanas, D. Garcia-Bernal, R. Garcia-Verdugo, L. Kremer, G. Marquez, and J. Teixido Intracellular signaling required for CCL25-stimulated T cell adhesion mediated by the integrin {alpha}4{beta}1 J. Leukoc. Biol., August 1, 2007; 82(2): 380 - 391. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Garcia-Bernal, E. Sotillo-Mallo, C. Nombela-Arrieta, R. Samaniego, Y. Fukui, J. V. Stein, and J. Teixido DOCK2 is Required for Chemokine-Promoted Human T Lymphocyte Adhesion Under Shear Stress Mediated by the Integrin {alpha}4beta1 J. Immunol., October 15, 2006; 177(8): 5215 - 5225. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Basu and H. E. Broxmeyer Transforming growth factor-{beta}1 modulates responses of CD34+ cord blood cells to stromal cell-derived factor-1/CXCL12 Blood, July 15, 2005; 106(2): 485 - 493. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Garcia-Bernal, N. Wright, E. Sotillo-Mallo, C. Nombela-Arrieta, J. V. Stein, X. R. Bustelo, and J. Teixido Vav1 and Rac Control Chemokine-promoted T Lymphocyte Adhesion Mediated by the Integrin {alpha}4{beta}1 Mol. Biol. Cell, July 1, 2005; 16(7): 3223 - 3235. [Abstract] [Full Text] [PDF]
|
|
