QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: E02-01-0056v1 14/1/93    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Togo, T. Articles by Steinhardt, R. A. Search for Related Content PubMed PubMed Citation Articles by Togo, T. Articles by Steinhardt, R. A.

Vol. 14, Issue 1, 93-106, January 2003

Long-Term Potentiation of Exocytosis and Cell Membrane Repair in Fibroblasts

Tatsuru Togo,^* Janet M. Alderton,^* and Richard A. Steinhardt

Department of Molecular and Cell Biology, University of California, Berkeley, California 94720-3200

We previously found that a microdisruption of the plasma membrane evokes Ca²⁺-regulated exocytosis near the wound site, which is essential for membrane resealing. We demonstrate herein that repeated membrane disruption reveals long-term potentiation of Ca²⁺-regulated exocytosis in 3T3 fibroblasts, which is closely correlated with faster membrane resealing rates. This potentiation of exocytosis is cAMP-dependent protein kinase A dependent in the early stages (minutes), in the intermediate term (hours) requires protein synthesis, and for long term (24 h) depends on the activation of cAMP response element-binding protein (CREB). We were able to demonstrate that wounding cells activated CREB within 3.5 h. In all three phases, the increase in the amount of exocytosis was correlated with an increase in the rate of membrane resealing. However, a brief treatment with forskolin, which is effective for short-term potentiation and which could also activate CREB, was not sufficient to induce long-term potentiation of resealing. These results imply that long-term potentiation by CREB required activation by another, cAMP-independent pathway.

---

^* Present address: Misaki Marine Biological Station, University of Tokyo, Misaki, Miura, Kanagawa 238-0225, Japan.

Corresponding author. E-mail address: rsteinha{at}socrates.berkeley.edu.

---

Molecular Biology of the Cell
Vol. 14, 93-106, January 2003
Copyright © 2003 by The American Society for Cell Biology

This article has been cited by other articles:

				A. Yaradanakul, T.-M. Wang, V. Lariccia, M.-J. Lin, C. Shen, X. Liu, and D. W. Hilgemann Massive Ca-induced Membrane Fusion and Phospholipid Changes Triggered by Reverse Na/Ca Exchange in BHK Fibroblasts J. Gen. Physiol., July 1, 2008; 132(1): 29 - 50. [Abstract] [Full Text] [PDF]

				K. Amano, K. Miyake, J.L. Borke, and P.L. McNeil Breaking Biological Barriers with a Toothbrush Journal of Dental Research, August 1, 2007; 86(8): 769 - 774. [Abstract] [Full Text] [PDF]

				T. Togo Disruption of the plasma membrane stimulates rearrangement of microtubules and lipid traffic toward the wound site J. Cell Sci., July 1, 2006; 119(13): 2780 - 2786. [Abstract] [Full Text] [PDF]

				N. E. Vlahakis and R. D. Hubmayr Cellular Stress Failure in Ventilator-injured Lungs Am. J. Respir. Crit. Care Med., June 15, 2005; 171(12): 1328 - 1342. [Abstract] [Full Text] [PDF]

				N. W. Andrews Membrane Resealing: Synaptotagmin VII Keeps Running the Show Sci. Signal., May 3, 2005; 2005(282): pe19 - pe19. [Abstract] [Full Text] [PDF]

				S. S. Shen, W. C. Tucker, E. R. Chapman, and R. A. Steinhardt Molecular Regulation of Membrane Resealing in 3T3 Fibroblasts J. Biol. Chem., January 14, 2005; 280(2): 1652 - 1660. [Abstract] [Full Text] [PDF]

				T. Togo Long-term Potentiation of Wound-induced Exocytosis and Plasma Membrane Repair Is Dependant on cAMP-response Element-mediated Transcription via a Protein Kinase C- and p38 MAPK-dependent Pathway J. Biol. Chem., October 22, 2004; 279(43): 44996 - 45003. [Abstract] [Full Text] [PDF]

				T. Togo and R. A. Steinhardt Nonmuscle Myosin IIA and IIB Have Distinct Functions in the Exocytosis-dependent Process of Cell Membrane Repair Mol. Biol. Cell, February 1, 2004; 15(2): 688 - 695. [Abstract] [Full Text] [PDF]