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Vol. 14, Issue 10, 4103-4113, October 2003
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* Institut de Biologie Cellulaire et de Morphologie, University of Lausanne, 1005 Lausanne, Switzerland;
Fukuda Initiative Research Unit, RIKEN, Saitama 351-0198, Japan;
Division de Biochimie Clinique, University of Geneva, Geneva 1211, Switzerland; and
Unité de Génétique des Déficits Sensoriels CNRS URA 1968, Institut Pasteur, Paris, France
Submitted January 20, 2003;
Revised June 10, 2003;
Accepted June 12, 2003
Monitoring Editor: Guido Guidotti
Rab27a is a GTPase associated with insulin-containing secretory granules of pancreatic 
-cells. Selective reduction of Rab27a expression by RNA interference did not alter granule distribution and basal secretion but impaired exocytosis triggered by insulin secretagogues. Screening for potential effectors of the GTPase revealed that the Rab27a-binding protein Slac2c/MyRIP is associated with secretory granules of -cells. Attenuation of Slac2c/MyRIP expression by RNA interference did not modify basal secretion but severely impaired hormone release in response to secretagogues. Although -cells express Myosin-Va, a potential partner of Slac2c/MyRIP, no functional link between the two proteins could be demonstrated. In fact, overexpression of the Myosin-Va binding domain of Slac2c/MyRIP did not affect granule localization and hormone exocytosis. In contrast, overexpression of the actin-binding domain of Slac2c/MyRIP led to a potent inhibition of exocytosis without detectable alteration in granule distribution. This effect was prevented by point mutations that abolish actin binding. Taken together our data suggest that Rab27a and Slac2c/MyRIP are part of a complex mediating the interaction of secretory granules with cortical actin cytoskeleton and participate to the regulation of the final steps of insulin exocytosis.
¶ Corresponding author. E-mail address: Romano.Regazzi{at}ibcm.unil.ch.
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