![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 14, Issue 10, 4316-4328, October 2003
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Institut für Physiologische Chemie, Ruhr-Universität Bochum, D-44780 Bochum, Germany
Submitted March 17, 2003;
Revised June 6, 2003;
Accepted June 10, 2003
Monitoring Editor: Howard Riezman
We describe the isolation and characterization of a homologous pair of proteins, Pex25p (YPL112c) and Pex27p (YOR193w), whose C-termini are similar to the entire Pex11p. All three proteins localize to the peroxisomal membrane and are likely to form homo-oligomers. Deletion of any of the three genes resulted in enlarged peroxisomes as revealed by fluorescence and electron microscopy. The partial growth defect on fatty acids of a pex25
mutant was not exacerbated by the additional deletion of PEX27; however, when PEX11 was deleted on top of that, growth was abolished on all fatty acids. Moreover, a severe peroxisomal protein import defect was observed in the pex11pex25pex27 triple mutant strain. This import defect was also observed when cells were grown on ethanol-containing medium, where peroxisomes are not required, suggesting that the function of the proteins in peroxisome biogenesis exceeds their role in proliferation. When Pex25p was overexpressed in the triple mutant strain, growth on oleic acid was completely restored and a massive proliferation of laminar membranes and peroxisomes was observed. Our data demonstrate that Pex11p, Pex25p, and Pex27p build a family of proteins whose members are required for peroxisome biogenesis and play a role in the regulation of peroxisome size and number.
Abbreviations used: MCFA, medium-chain fatty acids; PTS, peroxisomal targeting signal.
* These authors contributed equally to this work.
Present address: Amersham Bioscience, Freiburg, Germany.
Corresponding author. E-mail address: Ralf.Erdmann{at}ruhruni-bochum.de.
This article has been cited by other articles:
|
|
 |
|
  E. H. Hettema and A. M. Motley How peroxisomes multiply J. Cell Sci., July 15, 2009; 122(14): 2331 - 2336. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. J. Perry, F. D. Mast, and R. A. Rachubinski Endoplasmic Reticulum-Associated Secretory Proteins Sec20p, Sec39p, and Dsl1p Are Involved in Peroxisome Biogenesis Eukaryot. Cell, June 1, 2009; 8(6): 830 - 843. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. S. Escano, P. R. Juvvadi, F. J. Jin, T. Takahashi, Y. Koyama, S. Yamashita, J.-i. Maruyama, and K. Kitamoto Disruption of the Aopex11-1 Gene Involved in Peroxisome Proliferation Leads to Impaired Woronin Body Formation in Aspergillus oryzae Eukaryot. Cell, March 1, 2009; 8(3): 296 - 305. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Halbach, R. Rucktaschel, H. Rottensteiner, and R. Erdmann The N-domain of Pex22p Can Functionally Replace the Pex3p N-domain in Targeting and Peroxisome Formation J. Biol. Chem., February 6, 2009; 284(6): 3906 - 3916. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Rahim, S. Bischof, F. Kessler, and B. Agne In vivo interaction between atToc33 and atToc159 GTP-binding domains demonstrated in a plant split-ubiquitin system J. Exp. Bot., January 1, 2009; 60(1): 257 - 267. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. Lingard, S. K. Gidda, S. Bingham, S. J. Rothstein, R. T. Mullen, and R. N. Trelease Arabidopsis PEROXIN11c-e, FISSION1b, and DYNAMIN-RELATED PROTEIN3A Cooperate in Cell Cycle-Associated Replication of Peroxisomes PLANT CELL, June 1, 2008; 20(6): 1567 - 1585. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Yan, D. A. Rachubinski, S. Joshi, R. A. Rachubinski, and S. Subramani Dysferlin Domain-containing Proteins, Pex30p and Pex31p, Localized to Two Compartments, Control the Number and Size of Oleate-induced Peroxisomes in Pichia pastoris Mol. Biol. Cell, March 1, 2008; 19(3): 885 - 898. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Rubino, B. Navarro, and M. Russo Cymbidium ringspot virus defective interfering RNA replication in yeast cells occurs on endoplasmic reticulum-derived membranes in the absence of peroxisomes J. Gen. Virol., May 1, 2007; 88(5): 1634 - 1642. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Orth, S. Reumann, X. Zhang, J. Fan, D. Wenzel, S. Quan, and J. Hu The PEROXIN11 Protein Family Controls Peroxisome Proliferation in Arabidopsis PLANT CELL, January 1, 2007; 19(1): 333 - 350. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Schliebs, C. Wurtz, W.-H. Kunau, M. Veenhuis, and H. Rottensteiner A Eukaryote without Catalase-Containing Microbodies: Neurospora crassa Exhibits a Unique Cellular Distribution of Its Four Catalases. Eukaryot. Cell, September 1, 2006; 5(9): 1490 - 1502. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. J. Vizeacoumar, W. N. Vreden, M. Fagarasanu, G. A. Eitzen, J. D. Aitchison, and R. A. Rachubinski The Dynamin-like Protein Vps1p of the Yeast Saccharomyces cerevisiae Associates with Peroxisomes in a Pex19p-dependent Manner J. Biol. Chem., May 5, 2006; 281(18): 12817 - 12823. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Lingard and R. N. Trelease Five Arabidopsis peroxin 11 homologs individually promote peroxisome elongation, duplication or aggregation. J. Cell Sci., May 1, 2006; 119(Pt 9): 1961 - 1972. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Navarro, M. Russo, V. Pantaleo, and L. Rubino Cytological analysis of Saccharomyces cerevisiae cells supporting cymbidium ringspot virus defective interfering RNA replication. J. Gen. Virol., March 1, 2006; 87(Pt 3): 705 - 714. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Lay, B. L. Grosshans, H. Heid, K. Gorgas, and W. W. Just Binding and Functions of ADP-ribosylation Factor on Mammalian and Yeast Peroxisomes J. Biol. Chem., October 14, 2005; 280(41): 34489 - 34499. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Fagarasanu, A. Fagarasanu, Y. Y. C. Tam, J. D. Aitchison, and R. A. Rachubinski Inp1p is a peroxisomal membrane protein required for peroxisome inheritance in Saccharomyces cerevisiae J. Cell Biol., June 6, 2005; 169(5): 765 - 775. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Ano, T. Hattori, M. Oku, H. Mukaiyama, M. Baba, Y. Ohsumi, N. Kato, and Y. Sakai A Sorting Nexin PpAtg24 Regulates Vacuolar Membrane Dynamics during Pexophagy via Binding to Phosphatidylinositol-3-Phosphate Mol. Biol. Cell, February 1, 2005; 16(2): 446 - 457. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Marelli, J. J. Smith, S. Jung, E. Yi, A. I. Nesvizhskii, R. H. Christmas, R. A. Saleem, Y. Y. C. Tam, A. Fagarasanu, D. R. Goodlett, et al. Quantitative mass spectrometry reveals a role for the GTPase Rho1p in actin organization on the peroxisome membrane J. Cell Biol., December 20, 2004; 167(6): 1099 - 1112. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Koch, G. Schneider, G. H. Luers, and M. Schrader Peroxisome elongation and constriction but not fission can occur independently of dynamin-like protein 1 J. Cell Sci., September 1, 2004; 117(17): 3995 - 4006. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. J. Vizeacoumar, J. C. Torres-Guzman, D. Bouard, J. D. Aitchison, and R. A. Rachubinski Pex30p, Pex31p, and Pex32p Form a Family of Peroxisomal Integral Membrane Proteins Regulating Peroxisome Size and Number in Saccharomyces cerevisiae Mol. Biol. Cell, February 1, 2004; 15(2): 665 - 677. [Abstract] [Full Text] [PDF]
|
|
