![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 14, Issue 11, 4352-4364, November 2003
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
* Institut für Mikrobiologie und Genetik, Abteilung Molekulare Mikrobiologie, Universität Göttingen, D-37077 Göttingen, Germany;
School of Biological Sciences, University of Missouri-Kansas City, Kansas City, Missouri 64110
Submitted July 26, 2002;
Revised July 17, 2003;
Accepted July 17, 2003
Monitoring Editor: John Pringle
Cellular polarity is a fundamental property of every cell. Due to their extremely fast growth rate (
1 µm/s) and their highly elongated form, filamentous fungi represent a prime example of polarized growth and are an attractive model for the analysis of fundamental mechanisms underlying cellular polarity. To identify the critical components that contribute to polarized growth, we developed a large-scale genetic screen for the isolation of conditional mutants defective in this process in the model fungus Neurospora crassa. Phenotypic analysis and complementation tests of ca. 950 mutants identified more than 100 complementation groups that define 21 distinct morphological classes. The phenotypes include polarity defects over the whole hypha, more specific defects localized to hyphal tips or subapical regions, and defects in branch formation and growth directionality. To begin converting this mutant collection into meaningful biological information, we identified the defective genes in 45 mutants covering all phenotypic classes. These genes encode novel proteins as well as proteins which 1) regulate the actin or microtubule cytoskeleton, 2) are kinases or components of signal transduction pathways, 3) are part of the secretory pathway, or 4) have functions in cell wall formation or membrane biosynthesis. These findings highlight the dynamic nature of a fungal hypha and establish a molecular model for studies of hyphal growth and polarity.
Corresponding author. E-mail address: sseiler{at}gwdg.de. This article has been cited by other articles:
|
|
 |
|
  N. Vogt and S. Seiler The RHO1-specific GTPase-activating Protein LRG1 Regulates Polar Tip Growth in Parallel to Ndr Kinase Signaling in Neurospora Mol. Biol. Cell, November 1, 2008; 19(11): 4554 - 4569. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Kasuga and N. L. Glass Dissecting Colony Development of Neurospora crassa Using mRNA Profiling and Comparative Genomics Approaches Eukaryot. Cell, September 1, 2008; 7(9): 1549 - 1564. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. P. Semighini and S. D. Harris Regulation of Apical Dominance in Aspergillus nidulans Hyphae by Reactive Oxygen Species Genetics, August 1, 2008; 179(4): 1919 - 1932. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Li, L. Zhang, D. Wang, H. Zhou, H. Ouyang, J. Ming, and C. Jin Deletion of the msdS/AfmsdC gene induces abnormal polarity and septation in Aspergillus fumigatus Microbiology, July 1, 2008; 154(7): 1960 - 1972. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Chantret and S. E H Moore Free oligosaccharide regulation during mammalian protein N-glycosylation Glycobiology, March 1, 2008; 18(3): 210 - 224. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Meyer, R. A. Damveld, M. Arentshorst, U. Stahl, C. A. M. J. J. van den Hondel, and A. F. J. Ram Survival in the Presence of Antifungals: GENOME-WIDE EXPRESSION PROFILING OF ASPERGILLUS NIGER IN RESPONSE TO SUBLETHAL CONCENTRATIONS OF CASPOFUNGIN AND FENPROPIMORPH J. Biol. Chem., November 9, 2007; 282(45): 32935 - 32948. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-O. Park and E. Bi Central Roles of Small GTPases in the Development of Cell Polarity in Yeast and Beyond Microbiol. Mol. Biol. Rev., March 1, 2007; 71(1): 48 - 96. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Knechtle, J. Wendland, and P. Philippsen The SH3/PH Domain Protein AgBoi1/2 Collaborates with the Rho-Type GTPase AgRho3 To Prevent Nonpolar Growth at Hyphal Tips of Ashbya gossypii Eukaryot. Cell, October 1, 2006; 5(10): 1635 - 1647. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Seiler, N. Vogt, C. Ziv, R. Gorovits, and O. Yarden The STE20/Germinal Center Kinase POD6 Interacts with the NDR Kinase COT1 and Is Involved in Polar Tip Extension in Neurospora crassa Mol. Biol. Cell, September 1, 2006; 17(9): 4080 - 4092. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Bowman, A. Piwowar, M. Al Dabbous, J. Vierula, and S. J. Free Mutational Analysis of the Glycosylphosphatidylinositol (GPI) Anchor Pathway Demonstrates that GPI-Anchored Proteins Are Required for Cell Wall Biogenesis and Normal Hyphal Growth in Neurospora crassa Eukaryot. Cell, March 1, 2006; 5(3): 587 - 600. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-P. Schmitz, A. Kaufmann, M. Kohli, P. P. Laissue, and P. Philippsen From Function to Shape: A Novel Role of a Formin in Morphogenesis of the Fungus Ashbya gossypii Mol. Biol. Cell, January 1, 2006; 17(1): 130 - 145. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. R. Lew Mass flow and pressure-driven hyphal extension in Neurospora crassa Microbiology, August 1, 2005; 151(8): 2685 - 2692. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. D. Harris, N. D. Read, R. W. Roberson, B. Shaw, S. Seiler, M. Plamann, and M. Momany Polarisome Meets Spitzenkorper: Microscopy, Genetics, and Genomics Converge Eukaryot. Cell, February 1, 2005; 4(2): 225 - 229. [Full Text] [PDF]
|
|
|
|
|
|
S. Konzack, P. E. Rischitor, C. Enke, and R. Fischer The Role of the Kinesin Motor KipA in Microtubule Organization and Polarized Growth of Aspergillus nidulans Mol. Biol. Cell, February 1, 2005; 16(2): 497 - 506. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Bauer, P. Knechtle, J. Wendland, H. Helfer, and P. Philippsen A Ras-like GTPase Is Involved in Hyphal Growth Guidance in the Filamentous Fungus Ashbya gossypii Mol. Biol. Cell, October 1, 2004; 15(10): 4622 - 4632. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. B. Nichols, J. A. Fraser, and J. Heitman PAK Kinases Ste20 and Pak1 Govern Cell Polarity at Different Stages of Mating in Cryptococcus neoformans Mol. Biol. Cell, October 1, 2004; 15(10): 4476 - 4489. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. L. Pearson, K. Xu, K. E. Sharpless, and S. D. Harris MesA, a Novel Fungal Protein Required for the Stabilization of Polarity Axes in Aspergillus nidulans Mol. Biol. Cell, August 1, 2004; 15(8): 3658 - 3672. [Abstract] [Full Text] [PDF]
|
|
