![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 14, Issue 11, 4414-4426, November 2003
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
The Wellcome Centre for Molecular Parasitology, The University of Glasgow, Glasgow G11 6NU, United Kingdom
Submitted March 20, 2003;
Revised June 25, 2003;
Accepted June 26, 2003
Monitoring Editor: John Pringle
SEC-23 is a component of coat protein complex II (COPII)-coated vesicles involved in the endoplasmic reticulum-to-Golgi transport pathway of eukaryotes. During postembryonic life, Caenorhabditis elegans is surrounded by a collagenous exoskeleton termed the cuticle. From a screen for mutants defective in cuticle secretion, we identified and characterized a sec-23 mutant of C. elegans. By sequence homology, C. elegans has only the single sec-23 gene described herein. In addition to the cuticle secretion defect, mutants fail to complete embryonic morphogenesis. However, they progress through the earlier stages of embryogenesis, including gastrulation, and achieve substantial morphogenesis before death. We demonstrated a maternal component of SEC-23 function sufficient for progression through the earlier stages of embryogenesis and explaining the limited phenotype of the zygotic mutant. By RNA-mediated interference, we investigated the effects of perturbing COPII function during various postembryonic stages. During larval stages, major defects in cuticle synthesis and molting were observed. In the adult hermaphrodite, reduction of SEC-23 function by RNA-mediated interference caused a rapid onset of sterility, with defects in oogenesis including early maturation of the germline nuclei, probably a result of the observed loss of the GLP-1 receptor from the membrane surfaces adjacent to the developing germline nuclei.
This article has been cited by other articles:
|
|
 |
|
  D. Longman, R. H.A. Plasterk, I. L. Johnstone, and J. F. Caceres Mechanistic insights and identification of two novel factors in the C. elegans NMD pathway Genes & Dev., May 1, 2007; 21(9): 1075 - 1085. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Amanai, S. Shoji, N. Yoshida, M. Brahmajosyula, and A. C.F. Perry Injection of Mammalian Metaphase II Oocytes with Short Interfering RNAs to Dissect Meiotic and Early Mitotic Events Biol Reprod, December 1, 2006; 75(6): 891 - 898. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Liegeois, A. Benedetto, J.-M. Garnier, Y. Schwab, and M. Labouesse The V0-ATPase mediates apical secretion of exosomes containing Hedgehog-related proteins in Caenorhabditis elegans J. Cell Biol., June 19, 2006; 173(6): 949 - 961. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Novelli, A. P. Page, and J. Hodgkin The C Terminus of Collagen SQT-3 Has Complex and Essential Functions in Nematode Collagen Assembly Genetics, April 1, 2006; 172(4): 2253 - 2267. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Zugasti, J. Rajan, and P. E. Kuwabara The function and expansion of the Patched- and Hedgehog-related homologs in C. elegans Genome Res., October 1, 2005; 15(10): 1402 - 1410. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. P.-v. Berkel, M.H.G. Verheijen, E. Cuppen, M. Asahina, J. de Rooij, G. Jansen, R.H.A. Plasterk, J. L. Bos, and F.J.T. Zwartkruis Requirement of the Caenorhabditis elegans RapGEF pxf-1 and rap-1 for Epithelial Integrity Mol. Biol. Cell, January 1, 2005; 16(1): 106 - 116. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Britton and L. Murray Cathepsin L protease (CPL-1) is essential for yolk processing during embryogenesis in Caenorhabditis elegans J. Cell Sci., October 1, 2004; 117(21): 5133 - 5143. [Abstract] [Full Text] [PDF]
|
|
