![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 14, Issue 11, 4676-4684, November 2003
Department of Biochemistry, Molecular Biology and Cell Biology Northwestern University, Evanston, Illinois 60208
Submitted May 22, 2003;
Revised July 23, 2003;
Accepted July 24, 2003
Monitoring Editor: Benjamin Glick
Efficient internalization of proteins from the cell surface is essential for regulating cell growth and differentiation. In a screen for yeast mutants defective in ligand-stimulated internalization of the 
-factor receptor, we identified a mutant allele of TOR2, tor2G2128R. Tor proteins are known to function in translation initiation and nutrient sensing and are required for cell cycle progression through G1. Yeast Tor2 has an additional role in regulating the integrity of the cell wall by activating the Rho1 guanine nucleotide exchange factor Rom2. The endocytic defect in tor2G2128R cells is due to disruption of this Tor2 unique function. Other proteins important for cell integrity, Rom2 and the cell integrity sensor Wsc1, are also required for efficient endocytosis. A rho1 mutant specifically defective in activation of the glucan synthase Fks1/2 does not internalize -factor efficiently, and fks1
cells exhibit a similar phenotype. Removal of the cell wall does not inhibit internalization, suggesting that the function of Rho1 and Fks1 in endocytosis is not through cell wall synthesis or structural integrity. These findings reveal a novel function for the Tor2-Rho1 pathway in controlling endocytosis in yeast, a function that is mediated in part through the plasma membrane protein Fks1.
* Corresponding author. E-mail address: l-hicke{at}northwestern.edu.
This article has been cited by other articles:
|
|
 |
|
  S. A. Zurita-Martinez, R. Puria, X. Pan, J. D. Boeke, and M. E. Cardenas Efficient Tor Signaling Requires a Functional Class C Vps Protein Complex in Saccharomyces cerevisiae Genetics, August 1, 2007; 176(4): 2139 - 2150. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Aronova, K. Wedaman, S. Anderson, J. Yates III, and T. Powers Probing the Membrane Environment of the TOR Kinases Reveals Functional Interactions between TORC1, Actin, and Membrane Trafficking in Saccharomyces cerevisiae Mol. Biol. Cell, August 1, 2007; 18(8): 2779 - 2794. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-O. Park and E. Bi Central Roles of Small GTPases in the Development of Cell Polarity in Yeast and Beyond Microbiol. Mol. Biol. Rev., March 1, 2007; 71(1): 48 - 96. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Reinke, J. C.-Y. Chen, S. Aronova, and T. Powers Caffeine Targets TOR Complex I and Provides Evidence for a Regulatory Link between the FRB and Kinase Domains of Tor1p J. Biol. Chem., October 20, 2006; 281(42): 31616 - 31626. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Kamada, Y. Fujioka, N. N. Suzuki, F. Inagaki, S. Wullschleger, R. Loewith, M. N. Hall, and Y. Ohsumi Tor2 Directly Phosphorylates the AGC Kinase Ypk2 To Regulate Actin Polarization Mol. Cell. Biol., August 15, 2005; 25(16): 7239 - 7248. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Fadri, A. Daquinag, S. Wang, T. Xue, and J. Kunz The Pleckstrin Homology Domain Proteins Slm1 and Slm2 Are Required for Actin Cytoskeleton Organization in Yeast and Bind Phosphatidylinositol-4,5-Bisphosphate and TORC2 Mol. Biol. Cell, April 1, 2005; 16(4): 1883 - 1900. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Marelli, J. J. Smith, S. Jung, E. Yi, A. I. Nesvizhskii, R. H. Christmas, R. A. Saleem, Y. Y. C. Tam, A. Fagarasanu, D. R. Goodlett, et al. Quantitative mass spectrometry reveals a role for the GTPase Rho1p in actin organization on the peroxisome membrane J. Cell Biol., December 20, 2004; 167(6): 1099 - 1112. [Abstract] [Full Text] [PDF]
|
|
