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Originally published as MBC in Press, 10.1091/mbc.E03-05-0288 on August 7, 2003

Vol. 14, Issue 11, 4707-4720, November 2003

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Schizosaccharomyces pombe Rdh54 (TID1) Acts with Rhp54 (RAD54) to Repair Meiotic Double-Strand Breaks

Michael G. Catlett * {dagger}, and Susan L. Forsburg * {ddagger}

* Molecular & Cell Biology Laboratory, The Salk Institute, La Jolla, California 92037; {dagger} Division of Biology, University of California, San Diego, La Jolla, California 92093

Submitted May 8, 2003; Revised July 24, 2003; Accepted July 25, 2003
Monitoring Editor: Mark Solomon

We report the characterization of rdh54+, the second fission yeast Schizosaccharomyces pombe Rad54 homolog. rdh54+ shares sequence and functional homology to budding yeast RDH54/TID1. Rdh54p is present during meiosis with appropriate timing for a meiotic recombination factor. It interacts with Rhp51 and the meiotic Rhp51 homolog Dmc1 in yeast two-hybrid assays. Deletion of rdh54+ has no effect on DNA damage repair during the haploid vegetative cell cycle. In meiosis, however, rdh54{Delta} shows decreased spore viability and homologous recombination with a concomitant increase in sister chromatid exchange. The rdh54{Delta} single mutant repairs meiotic breaks with similar timing to wild type, suggesting redundancy of meiotic recombination factors. Consistent with this, the rdh54{Delta} rhp54{Delta} double mutant fails to repair meiotic double strand breaks. Live cell analysis shows that rdh54{Delta} rhp54{Delta} asci do not arrest, but undergo both meiotic divisions with near normal timing, suggesting that failure to repair double strand breaks in S. pombe meiosis does not result in checkpoint arrest.

Online version of this article contains video material for some figures. Online version is available at www.molbiolcell.org.

§ Corresponding author. E-mail address: forsburg{at}salk.edu.




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