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Vol. 14, Issue 12, 4758-4769, December 2003

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Glom Is a Novel Mitochondrial DNA Packaging Protein in Physarum polycephalum and Causes Intense Chromatin Condensation without Suppressing DNA Functions

Narie Sasaki ^* , Haruko Kuroiwa , Chikako Nishitani , Hiroyoshi Takano ^¶, Tetsuya Higashiyama , Tamaki Kobayashi , Yuki Shirai ^*, Atsushi Sakai ^||, Shigeyuki Kawano ^#, Kimiko Murakami-Murofushi ^*, and Tsuneyoshi Kuroiwa 

^* Department of Biology, Faculty of Science, Ochanomizu University, Tokyo, Japan; Department of Life Science, College of Science, Rikkyo (St. Paul's) University, Tokyo, Japan; Department of Biological Sciences, Graduate School of Science, University of Tokyo, Tokyo, Japan; ^¶ Department of Biological Science, Faculty of Science, Kumamoto University, Kumamoto, Japan; ^|| Department of Biology, Faculty of Science, Nara Women's University, Nara, Japan; and ^# Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Chiba, Japan

Submitted February 21, 2003; Revised August 5, 2003; Accepted August 10, 2003
Monitoring Editor: Thomas Fox

Mitochondrial DNA (mtDNA) is packed into highly organized structures called mitochondrial nucleoids (mt-nucleoids). To understand the organization of mtDNA and the overall regulation of its genetic activity within the mt-nucleoids, we identified and characterized a novel mtDNA packaging protein, termed Glom (a protein inducing agglomeration of mitochondrial chromosome), from highly condensed mt-nucleoids of the true slime mold, Physarum polycephalum. This protein could bind to the entire mtDNA and package mtDNA into a highly condensed state in vitro. Immunostaining analysis showed that Glom specifically localized throughout the mt-nucleoid. Deduced amino acid sequence revealed that Glom has a lysine-rich region with proline-rich domain in the N-terminal half and two HMG boxes in C-terminal half. Deletion analysis of Glom revealed that the lysine-rich region was sufficient for the intense mtDNA condensation in vitro. When the recombinant Glom proteins containing the lysine-rich region were expressed in Escherichia coli, the condensed nucleoid structures were observed in E. coli. Such in vivo condensation did not interfere with transcription or replication of E. coli chromosome and the proline-rich domain was essential to keep those genetic activities. The expression of Glom also complemented the E. coli mutant lacking the bacterial histone-like protein HU and the HMG-boxes region of Glom was important for the complementation. Our results suggest that Glom is a new mitochondrial histone-like protein having a property to cause intense DNA condensation without suppressing DNA functions.

Corresponding author. E-mail address: narie{at}cc.ocha.ac.jp.

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