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Vol. 14, Issue 12, 4805-4812, December 2003
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* Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605;
Department of Physiology, University of Massachusetts Medical School, Worcester, Massachusetts 01605; and
Program in Cell Dynamics, University of Massachusetts Medical School, Worcester, Massachusetts 01605
Submitted June 12, 2003;
Revised July 18, 2003;
Accepted August 8, 2003
Monitoring Editor: Joseph Gall
Although the complex process of ribosome assembly in the nucleolus is beginning to be understood, little is known about how the ribosomal subunits move from the nucleolus to the nuclear membrane for transport to the cytoplasm. We show here that large ribosomal subunits move out from the nucleolus and into the nucleoplasm in all directions, with no evidence of concentrated movement along directed paths. Mobility was slowed compared with that expected in aqueous solution in a manner consistent with anomalous diffusion. Once nucleoplasmic, the subunits moved in the same random manner and also sometimes visited another nucleolus before leaving the nucleus.
Online version of this article contains video material for some figures. Online version is available at www.molbiolcell.org.
Corresponding author. E-mail address: joan.politz{at}umassmed.edu.
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