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Vol. 14, Issue 12, 4885-4895, December 2003
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to the Plasma Membrane in RBL-2H3 Cells
Department de Bioquímica y Biología Molecular (A), Facultad de Veterinaria, Universidad de Murcia, Apdo. 4021, E-30100 Murcia, Spain
Submitted May 13, 2003;
Revised July 3, 2003;
Accepted August 6, 2003
Monitoring Editor: Carl Henrik-Heldin
To evaluate the role of the C2 domain in protein kinase C
(PKC) localization and activation after stimulation of the IgE receptor in RBL-2H3 cells, we used a series of mutants located in the phospholipid binding region of the enzyme. The results obtained suggest that the interaction of the C2 domain with the phospholipids in the plasma membrane is essential for anchoring the enzyme in this cellular compartment. Furthermore, the use of specific inhibitors of the different pathways that generate both diacylglycerol and phosphatidic acid has shown that the phosphatidic acid generated via phospholipase D (PLD)-dependent pathway, in addition to the diacylglycerol generated via phosphoinosite-phospholipase C (PLC), are involved in the localization of PKC in the plasma membrane. Direct stimulation of RBL-2H3 cells with very low concentrations of permeable phosphatidic acid and diacylglycerol exerted a synergistic effect on the plasma membrane localization of PKC. Moreover, the in vitro kinase assays showed that both phosphatidic acid and diacylglycerol are essential for enzyme activation. Together, these results demonstrate that phosphatidic acid is an important and essential activator of PKC through the C2 domain and locate this isoenzyme in a new scenario where it acts as a downstream target of PLD.
Abbreviations used: DAG, diacylglycerol; DGK, diacylglycerol kinase; DiC8, 1,2-dioctanoylglycerol; DNP-HSA, dinitrophenyl-human serum albumin; PC-PLC, phosphatidylcholine-phospholipase C; PI-PLC, phosphoinosite-phospholipase C; PIP5, phosphatidylinositol 4-phosphate 5-kinase; PLD, phospholipase D; PtdOH, phosphatidic acid; POPA, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphate.
* Corresponding author. E-mail address: senena{at}um.es.
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