Molecular Biology of the Cell click for CBE Life Science Education Page

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

Originally published as MBC in Press, 10.1091/mbc.E03-01-0025 on October 17, 2003

Vol. 14, Issue 12, 5089-5097, December 2003

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
E03-01-0025v1
14/12/5089    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by King, S. J.
Right arrow Articles by Schroer, T. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by King, S. J.
Right arrow Articles by Schroer, T. A.

Analysis of the Dynein-Dynactin Interaction In Vitro and In Vivo

Stephen J. King * {dagger}, Christa L. Brown *, Kerstin C. Maier, Nicholas J. Quintyne {ddagger}, and Trina A. Schroer §

Department of Biology, The Johns Hopkins University, Baltimore, Maryland 21218

Submitted January 20, 2003; Revised August 7, 2003; Accepted August 18, 2003
Monitoring Editor: Lawrence Goldstein

Cytoplasmic dynein and dynactin are megadalton-sized multisubunit molecules that function together as a cytoskeletal motor. In the present study, we explore the mechanism of dynein-dynactin binding in vitro and then extend our findings to an in vivo context. Solution binding assays were used to define binding domains in the dynein intermediate chain (IC) and dynactin p150Glued subunit. Transient overexpression of a series of fragments of the dynein IC was used to determine the importance of this subunit for dynein function in mammalian tissue culture cells. Our results suggest that a functional dynein-dynactin interaction is required for proper microtubule organization and for the transport and localization of centrosomal components and endomembrane compartments. The dynein IC fragments have different effects on endomembrane localization, suggesting that different endomembranes may bind dynein via distinct mechanisms.

* These authors contributed equally to this work.

{dagger} Present addresses:Division of Molecular Biology and Biochemistry, School of Biological Sciences, University of Missouri-Kansas City, Kansas City, MO 64110;

{ddagger} Department of Biology, University of Pittsburgh, 258 Crawford Hall, Pittsburgh, PA 15260.

§ Corresponding author. E-mail address: schroer{at}jhu.edu.




This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
J. R. Kardon, S. L. Reck-Peterson, and R. D. Vale
Regulation of the processivity and intracellular localization of Saccharomyces cerevisiae dynein by dynactin
PNAS, April 7, 2009; 106(14): 5669 - 5674.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
E. Ai, D. S. Poole, and A. R. Skop
RACK-1 Directs Dynactin-dependent RAB-11 Endosomal Recycling during Mitosis in Caenorhabditis elegans
Mol. Biol. Cell, March 15, 2009; 20(6): 1629 - 1638.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
N. B. Martin-Cofreces, J. Robles-Valero, J. R. Cabrero, M. Mittelbrunn, M. Gordon-Alonso, C.-H. Sung, B. Alarcon, J. Vazquez, and F. Sanchez-Madrid
MTOC translocation modulates IS formation and controls sustained T cell signaling
J. Cell Biol., September 9, 2008; 182(5): 951 - 962.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
M. Uteng, C. Hentrich, K. Miura, P. Bieling, and T. Surrey
Poleward transport of Eg5 by dynein-dynactin in Xenopus laevis egg extract spindles
J. Cell Biol., August 25, 2008; 182(4): 715 - 726.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
H. Si, S. C. Verma, M. A. Lampson, Q. Cai, and E. S. Robertson
Kaposi's Sarcoma-Associated Herpesvirus-Encoded LANA Can Interact with the Nuclear Mitotic Apparatus Protein To Regulate Genome Maintenance and Segregation
J. Virol., July 1, 2008; 82(13): 6734 - 6746.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
N. P. Ferenz and P. Wadsworth
Prophase Microtubule Arrays Undergo Flux-like Behavior in Mammalian Cells
Mol. Biol. Cell, October 1, 2007; 18(10): 3993 - 4002.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. A. Melkonian, K. C. Maier, J. E. Godfrey, M. Rodgers, and T. A. Schroer
Mechanism of Dynamitin-mediated Disruption of Dynactin
J. Biol. Chem., July 6, 2007; 282(27): 19355 - 19364.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
H. Kim, S.-C. Ling, G. C. Rogers, C. Kural, P. R. Selvin, S. L. Rogers, and V. I. Gelfand
Microtubule binding by dynactin is required for microtubule organization but not cargo transport
J. Cell Biol., February 26, 2007; 176(5): 641 - 651.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Zhao, I. Gaidarov, and J. H. Keen
Phosphoinositide 3-Kinase C2{alpha} Links Clathrin to Microtubule-dependent Movement
J. Biol. Chem., January 12, 2007; 282(2): 1249 - 1256.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
J. Gaetz and T. M. Kapoor
Dynein/dynactin regulate metaphase spindle length by targeting depolymerizing activities to spindle poles
J. Cell Biol., August 16, 2004; 166(4): 465 - 471.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
J. J. Baggett, K. E. D'Aquino, and B. Wendland
The Sla2p Talin Domain Plays a Role in Endocytosis in Saccharomyces cerevisiae
Genetics, December 1, 2003; 165(4): 1661 - 1674.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Copyright © 2003 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.