![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Vol. 14, Issue 2, 445-459, February 2003
*Departament de Biologia Cel.lular i Anatomia
Patològica, Facultat de Medicina, Institut d'Investigacions
Biomèdiques August Pi i Sunyer, Universitat de Barcelona, E-08036
Barcelona, Spain; We have previously reported that actin filaments are involved in
protein transport from the Golgi complex to the endoplasmic reticulum.
Herein, we examined whether myosin motors or actin comets mediate this
transport. To address this issue we have used, on one hand, a
combination of specific inhibitors such as 2,3-butanedione monoxime
(BDM) and 1-[5-isoquinoline sulfonyl]-2-methyl piperazine (ML7),
which inhibit myosin and the phosphorylation of myosin II by the myosin
light chain kinase, respectively; and a mutant of the nonmuscle myosin
II regulatory light chain, which cannot be phosphorylated
(MRLC2AA). On the other hand, actin comet tails were
induced by the overexpression of phosphatidylinositol phosphate
5-kinase. Cells treated with BDM/ML7 or those that express the
MRLC2AA mutant revealed a significant reduction in the
brefeldin A (BFA)-induced fusion of Golgi enzymes with the endoplasmic
reticulum (ER). This delay was not caused by an alteration in the
formation of the BFA-induced tubules from the Golgi complex. In
addition, the Shiga toxin fragment B transport from the Golgi complex
to the ER was also altered. This impairment in the retrograde protein
transport was not due to depletion of intracellular calcium stores or
to the activation of Rho kinase. Neither the reassembly of the Golgi complex after BFA removal nor VSV-G transport from ER to the Golgi was
altered in cells treated with BDM/ML7 or expressing
MRLC2AA. Finally, transport carriers containing Shiga toxin
did not move into the cytosol at the tips of comet tails of
polymerizing actin. Collectively, the results indicate that 1) myosin
motors move to transport carriers from the Golgi complex to the ER
along actin filaments; 2) nonmuscle myosin II mediates in this process;
and 3) actin comets are not involved in retrograde transport.
Serveis
CientificoTècnics, Universitat de Barcelona, E-08028 Barcelona,
Spain; §School of Biosciences, Molecular Cell
Division, University of Birmingham, Birmingham B15-2TT, United
Kingdom; Centro de Investigación,
Hospital La Fe, E-46009 Valencia, Spain;
¶Department of Biological Science, Graduate
School of Science, Hiroshima University, 739-8526 Hiroshima, Japan; and
#Department of Biology, University of California
San Diego, La Jolla, California 92093
Online
version of this article contains supplementary data. Online version
available at www.molbiolcell.org.
Imperial Cancer Research Fund, 44 Lincoln's Inn
Fields, London WC2A 3PX, England.
@
Corresponding author. E-mail address:
egea{at}medicina.ub.es.
This article has been cited by other articles:
|
|
 |
|
  J. A. Wasylnka, M. A. Bakowski, J. Szeto, M. B. Ohlson, W. S. Trimble, S. I. Miller, and J. H. Brumell Role for Myosin II in Regulating Positioning of Salmonella-Containing Vacuoles and Intracellular Replication Infect. Immun., June 1, 2008; 76(6): 2722 - 2735. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. J. Guerriero and O. A. Weisz N-WASP inhibitor wiskostatin nonselectively perturbs membrane transport by decreasing cellular ATP levels Am J Physiol Cell Physiol, April 1, 2007; 292(4): C1562 - C1566. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Betapudi, L. S. Licate, and T. T. Egelhoff Distinct Roles of Nonmuscle Myosin II Isoforms in the Regulation of MDA-MB-231 Breast Cancer Cell Spreading and Migration. Cancer Res., May 1, 2006; 66(9): 4725 - 4733. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Steet and S. Kornfeld COG-7-deficient Human Fibroblasts Exhibit Altered Recycling of Golgi Proteins Mol. Biol. Cell, May 1, 2006; 17(5): 2312 - 2321. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Xu and M. Stamnes The Actin-depolymerizing Factor Homology and Charged/Helical Domains of Drebrin and mAbp1 Direct Membrane Binding and Localization via Distinct Interactions with Actin J. Biol. Chem., April 28, 2006; 281(17): 11826 - 11833. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. V. Jerdeva, K. Wu, F. A. Yarber, C. J. Rhodes, D. Kalman, J. E. Schechter, and S. F. Hamm-Alvarez Actin and non-muscle myosin II facilitate apical exocytosis of tear proteins in rabbit lacrimal acinar epithelial cells J. Cell Sci., October 15, 2005; 118(20): 4797 - 4812. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-L. Chen, R. V. Fucini, L. Lacomis, H. Erdjument-Bromage, P. Tempst, and M. Stamnes Coatomer-bound Cdc42 regulates dynein recruitment to COPI vesicles J. Cell Biol., May 9, 2005; 169(3): 383 - 389. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Betapudi, C. Mason, L. Licate, and T. T. Egelhoff Identification and Characterization of a Novel {alpha}-Kinase with a von Willebrand Factor A-like Motif Localized to the Contractile Vacuole and Golgi Complex in Dictyostelium discoideum Mol. Biol. Cell, May 1, 2005; 16(5): 2248 - 2262. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Azorin, M. Portoles, P. Marin, F. Lazaro-Dieguez, L. Megias, G. Egea, and J. Renau-Piqueras PRENATAL ETHANOL EXPOSURE ALTERS THE CYTOSKELETON AND INDUCES GLYCOPROTEIN MICROHETEROGENEITY IN RAT NEWBORN HEPATOCYTES Alcohol Alcohol., May 1, 2004; 39(3): 203 - 212. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Lalli, S. Gschmeissner, and G. Schiavo Myosin Va and microtubule-based motors are required for fast axonal retrograde transport of tetanus toxin in motor neurons J. Cell Sci., November 15, 2003; 116(22): 4639 - 4650. [Abstract] [Full Text] [PDF]
|
|
