QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: E02-09-0556v1 14/2/698    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hasegawa, H. Articles by Hay, J. C. Search for Related Content PubMed PubMed Citation Articles by Hasegawa, H. Articles by Hay, J. C.

Vol. 14, Issue 2, 698-720, February 2003

Mammalian Ykt6 Is a Neuronal SNARE Targeted to a Specialized Compartment by its Profilin-like Amino Terminal Domain

Haruki Hasegawa,^* Sara Zinsser,^* Yeyoung Rhee,^* Einar Osland Vik-Mo, Svend Davanger, and Jesse C. Hay^*

 ^*University of Michigan, Department of Molecular, Cellular, and Developmental Biology, Ann Arbor, Michigan 48109-1048; and  University of Bergen, Department of Anatomy and Cell Biology, and Locus on Neuroscience, Bergen, Norway

SNAREs are required for specific membrane fusion throughout the endomembrane system. Here we report the characterization of rat ykt6, a prenylated SNARE selectively expressed in brain neurons. Immunofluorescence microscopy in neuronal and neuroendocrine cell lines revealed that membrane-associated ykt6 did not colocalize significantly with any conventional markers of endosomes, lysosomes, or the secretory pathway. However, ykt6-containing membranes displayed very minor overlaps with lysosomes and dense-core secretory granules and were similar to lysosomes in buoyant density. Thus, ykt6 appears to be specialized for the trafficking of a unique membrane compartment, perhaps related to lysosomes, involved in aspects of neuronal function. Targeting of this SNARE to the ykt6 compartment was mediated by its profilin-like amino-terminal domain, even in the absence of protein prenylation. Although several other R-SNAREs contain related amino-terminal domains, only the ykt6 version was able to confer the specialized localization. Rat ykt6, which contains an arginine in its SNARE motif zero-layer, was found to behave like other R-SNAREs in its SNARE assembly properties. Interestingly, cytosolic ykt6, constituting more than half of the total cellular pool, appeared to be conformationally inactive for SNARE complex assembly, perhaps indicative of a regulatory mechanism that prevents promiscuous and potentially deleterious SNARE interactions.

---

Corresponding author. E-mail address: jessehay{at}umich.edu.

---

Molecular Biology of the Cell
Vol. 14, 698-720, February 2003
Copyright © 2003 by The American Society for Cell Biology

This article has been cited by other articles:

				P. Moreau, F. Brandizzi, S. Hanton, L. Chatre, S. Melser, C. Hawes, and B. Satiat-Jeunemaitre The plant ER-Golgi interface: a highly structured and dynamic membrane complex J. Exp. Bot., January 1, 2007; 58(1): 49 - 64. [Abstract] [Full Text] [PDF]

				S. A. Siddiqi, S. Siddiqi, J. Mahan, K. Peggs, F. S. Gorelick, and C. M. Mansbach II The Identification of a Novel Endoplasmic Reticulum to Golgi SNARE Complex Used by the Prechylomicron Transport Vesicle J. Biol. Chem., July 28, 2006; 281(30): 20974 - 20982. [Abstract] [Full Text] [PDF]

				S. A. Siddiqi, J. Mahan, S. Siddiqi, F. S. Gorelick, and C. M. Mansbach II Vesicle-associated membrane protein 7 is expressed in intestinal ER. J. Cell Sci., March 1, 2006; 119(Pt 5): 943 - 950. [Abstract] [Full Text] [PDF]

				H. Hasegawa, Z. Yang, L. Oltedal, S. Davanger, and J. C. Hay Intramolecular protein-protein and protein-lipid interactions control the conformation and subcellular targeting of neuronal Ykt6 J. Cell Sci., September 1, 2004; 117(19): 4495 - 4508. [Abstract] [Full Text] [PDF]

				Y. Liu, J. J. Flanagan, and C. Barlowe Sec22p Export from the Endoplasmic Reticulum Is Independent of SNARE Pairing J. Biol. Chem., June 25, 2004; 279(26): 27225 - 27232. [Abstract] [Full Text] [PDF]

				M. Fukasawa, O. Varlamov, W. S. Eng, T. H. Sollner, and J. E. Rothman Localization and activity of the SNARE Ykt6 determined by its regulatory domain and palmitoylation PNAS, April 6, 2004; 101(14): 4815 - 4820. [Abstract] [Full Text] [PDF]

				H. Nakanishi, P. de los Santos, and A. M. Neiman Positive and Negative Regulation of a SNARE Protein by Control of Intracellular Localization Mol. Biol. Cell, April 1, 2004; 15(4): 1802 - 1815. [Abstract] [Full Text] [PDF]

				A. Volchuk, M. Ravazzola, A. Perrelet, W. S. Eng, M. Di Liberto, O. Varlamov, M. Fukasawa, T. Engel, T. H. Sollner, J. E. Rothman, et al. Countercurrent Distribution of Two Distinct SNARE Complexes Mediating Transport within the Golgi Stack Mol. Biol. Cell, April 1, 2004; 15(4): 1506 - 1518. [Abstract] [Full Text] [PDF]

				R. Kuliawat, E. Kalinina, J. Bock, L. Fricker, T. E. McGraw, S. R. Kim, J. Zhong, R. Scheller, and P. Arvan Syntaxin-6 SNARE Involvement in Secretory and Endocytic Pathways of Cultured Pancreatic {beta}-Cells Mol. Biol. Cell, April 1, 2004; 15(4): 1690 - 1701. [Abstract] [Full Text] [PDF]

				S. Martinez-Arca, R. Rudge, M. Vacca, G. Raposo, J. Camonis, V. Proux-Gillardeaux, L. Daviet, E. Formstecher, A. Hamburger, F. Filippini, et al. A dual mechanism controlling the localization and function of exocytic v-SNAREs PNAS, July 22, 2003; 100(15): 9011 - 9016. [Abstract] [Full Text] [PDF]

				A. P. Joglekar, D. Xu, D. J. Rigotti, R. Fairman, and J. C. Hay The SNARE Motif Contributes to rbet1 Intracellular Targeting and Dynamics Independently of SNARE Interactions J. Biol. Chem., April 11, 2003; 278(16): 14121 - 14133. [Abstract] [Full Text] [PDF]