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Vol. 14, Issue 2, 721-729, February 2003

A Truncated Form of KlLsm4p and the Absence of Factors Involved in mRNA Decapping Trigger Apoptosis in Yeast

Cristina Mazzoni,^* Patrizia Mancini,^* Loredana Verdone,^§ Frank Madeo, Agnese Serafini,^* Eva Herker, and Claudio Falcone^*

 ^*Pasteur Institute-Cenci Bolognetti Foundation, Department of Cell and Developmental Biology, University of Rome, 00185 Rome, Italy;  Department of Experimental Medicine and Pathology, University of Rome, 00161 Rome, Italy;  ^§ Department of Genetic and Molecular Biology, University of Rome, 00185 Rome, Italy; and  Physiologish-chemisches Institut, Universität Tübingen, 72076 Tübingen, Germany

The LSM4 gene of Saccharomyces cerevisiae codes for an essential protein involved in pre-mRNA splicing and also in mRNA decapping, a crucial step for mRNA degradation. We previously demonstrated that the first 72 amino acids of the Kluyveromyces lactis Lsm4p (KlLsm4p), which contain the Sm-like domains, can restore cell viability in both K. lactis and S. cerevisiae cells not expressing the endogenous protein. However, the absence of the carboxy-terminal region resulted in a remarkable loss of viability in stationary phase cells (Mazzoni and Falcone, 2001). Herein, we demonstrate that S. cerevisiae cells expressing the truncated LSM4 protein of K. lactis showed the phenotypic markers of yeast apoptosis such as chromatin condensation, DNA fragmentation, and accumulation of reactive oxygen species. The study of deletion mutants revealed that apoptotic markers were clearly evident also in strains lacking genes involved in mRNA decapping, such as LSM1, DCP1, and DCP2, whereas a slight effect was observed in strains lacking the genes DHH1 and PAT1. This is the first time that a connection between mRNA stability and apoptosis is reported in yeast, pointing to mRNA decapping as the crucial step responsible of the observed apoptotic phenotypes.

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Corresponding author. E-mail address: cristina.mazzoni{at}uniroma1.it.

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Molecular Biology of the Cell
Vol. 14, 721-729, February 2003
Copyright © 2003 by The American Society for Cell Biology

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