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Originally published as MBC in Press, 10.1091/mbc.E02-06-0314 on November 18, 2002
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Vol. 14, Issue 2, 730-747, February 2003

Cdc50p, a Conserved Endosomal Membrane Protein, Controls Polarized Growth in Saccharomyces cerevisiae

Kenjiro Misu,*dagger Konomi Fujimura-Kamada,* Takashi Ueda,Dagger Akihiko Nakano,Dagger Hiroyuki Katoh,dagger and Kazuma Tanaka*§

 *Division of Molecular Interaction, Institute for Genetic Medicine and  dagger Surgical Oncology, Division of Cancer Medicine, Hokkaido University Graduate School of Medicine, N15 W7, Kita-ku, Sapporo, 060-0815, Japan; and  Dagger Molecular Membrane Biology Laboratory, Riken, 2-1 Wako, Saitama 351-0198, Japan

During the cell cycle of the yeast Saccharomyces cerevisiae, the actin cytoskeleton and the growth of cell surface are polarized, mediating bud emergence, bud growth, and cytokinesis. We identified CDC50 as a multicopy suppressor of the myo3 myo5-360 temperature-sensitive mutant, which is defective in organization of cortical actin patches. The cdc50 null mutant showed cold-sensitive cell cycle arrest with a small bud as reported previously. Cortical actin patches and Myo5p, which are normally localized to polarization sites, were depolarized in the cdc50 mutant. Furthermore, actin cables disappeared, and Bni1p and Gic1p, effectors of the Cdc42p small GTPase, were mislocalized in the cdc50 mutant. As predicted by its amino acid sequence, Cdc50p appears to be a transmembrane protein because it was solubilized from the membranes by detergent treatment. Cdc50p colocalized with Vps21p in endosomal compartments and was also localized to the class E compartment in the vps27 mutant. The cdc50 mutant showed defects in a late stage of endocytosis but not in the internalization step. It showed, however, only modest defects in vacuolar protein sorting. Our results indicate that Cdc50p is a novel endosomal protein that regulates polarized cell growth.

§ Corresponding author. E-mail address: k-tanaka{at}med.hokudai.ac.jp.

Molecular Biology of the Cell
Vol. 14, 730-747, February 2003
Copyright © 2003 by The American Society for Cell Biology


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