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Vol. 14, Issue 3, 1043-1057, March 2003
Cold Spring Harbor Laboratory, Cold Spring Harbor, New
York 11724
In eukaryotic cells, RNA polymerase II (RNA pol II) transcription
and pre-mRNA processing are coordinated events. We have addressed how
individual components of the transcription and pre-mRNA processing
machinery are organized during mitosis and subsequently recruited into
the newly formed daughter nuclei. Interestingly, localization studies
of numerous RNA pol II transcription and pre-mRNA processing factors
revealed a nonrandom and sequential entry of these factors into
daughter nuclei after nuclear envelope/lamina formation. The initiation
competent form of RNA pol II and general transcription factors appeared
in the daughter nuclei simultaneously, but prior to pre-mRNA processing
factors, whereas the elongation competent form of RNA pol II was
detected even later. The differential entry of these factors rules out
the possibility that they are transported as a unitary complex.
Telophase nuclei were competent for transcription and pre-mRNA splicing
concomitant with the initial entry of the respective factors. In
addition, our results revealed a low turnover rate of transcription and
pre-mRNA splicing factors during mitosis. We provide evidence to
support a model in which the entry of the RNA pol II gene expression
machinery into newly forming daughter nuclei is a staged and ordered process.
Online version of this article contains video material for some
figures. Online version available at www.molbiolcell.org.
*
Corresponding author. E-mail address: spector{at}cshl.org.
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