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Vol. 14, Issue 3, 1058-1073, March 2003
*University of California, Berkeley, California 94720-3200;
The striped bass has two retina-expressed class III myosin genes,
each composed of a kinase, motor, and tail domain. We report the
cloning, sequence analysis, and expression patterns of the long (Myo3A)
and short (Myo3B) class III myosins, as well as cellular localization
and biochemical characterization of the long isoform, Myo3A. Myo3A (209 kDa) is expressed in the retina, brain, testis, and sacculus, and Myo3B
(155 kDa) is expressed in the retina, intestine, and testis. The tails
of these two isoforms contain two highly conserved domains, 3THDI and
3THDII. Whereas Myo3B has three IQ motifs, Myo3A has nine IQ motifs,
four in its neck and five in its tail domain. Myo3A localizes to actin
filament bundles of photoreceptors and is concentrated in the calycal
processes. An anti-Myo3A antibody decorates the actin
cytoskeleton of rod inner/outer segments, and this labeling is reduced
by the presence of ATP. The ATP-sensitive actin association is a
feature characteristic of myosin motors. The numerous IQ motifs may
play a structural or signaling role in the Myo3A, and its localization
to calycal processes indicates that this myosin mediates a local
function at this site in vertebrate photoreceptors.
Bayer Pharmaceutical Division, Berkeley,
California 94701-1986; University of the Pacific
School of Pharmacy, Stockton, California 95211; and
§Ingenuity Inc., Mountain View, California
94043
Corresponding author. E-mail address:
acdose{at}socrates.berkeley.edu.
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