Molecular Biology of the Cell track citations

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

Originally published as MBC in Press, 10.1091/mbc.E02-07-0425 on December 7, 2002
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
E02-07-0425v1
14/3/1097    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zhou, R.
Right arrow Articles by Yao, X.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhou, R.
Right arrow Articles by Yao, X.

Vol. 14, Issue 3, 1097-1108, March 2003

Polarized Distribution of IQGAP Proteins in Gastric Parietal Cells and Their Roles in Regulated Epithelial Cell Secretion

Rihong Zhou,*dagger Dagger Zhen Guo,*dagger Charles Watson,Dagger Emily Chen,Dagger Rong Kong,dagger Wenxian Wang,dagger and Xuebiao Yaodagger Dagger §

 dagger Laboratory of Cell Dynamics, School of Life Sciences, University of Science and Technology of China, Hefei, China 230027; and  Dagger Department of Molecular and Cell Biology, University of California, Berkeley, California 94720

Actin cytoskeleton plays an important role in the establishment of epithelial cell polarity. Cdc42, a member of Rho GTPase family, modulates actin dynamics via its regulators, such as IQGAP proteins. Gastric parietal cells are polarized epithelial cells in which regulated acid secretion occurs in the apical membrane upon stimulation. We have previously shown that actin isoforms are polarized to different membrane domains and that the integrity of the actin cytoskeleton is essential for acid secretion. Herein, we show that Cdc42 is preferentially distributed to the apical membrane of gastric parietal cells. In addition, we revealed that two Cdc42 regulators, IQGAP1 and IQGAP2, are present in gastric parietal cells. Interestingly, IQGAP2 is polarized to the apical membrane of the parietal cells, whereas IQGAP1 is mainly distributed to the basolateral membrane. An IQGAP peptide that competes with full-length IQGAP proteins for Cdc42-binding in vitro also inhibits acid secretion in streptolysin-O-permeabilized gastric glands. Furthermore, this peptide disrupts the association of IQGAP and Cdc42 with the apical actin cytoskeleton and prevents the apical membrane remodeling upon stimulation. We propose that IQGAP2 forms a link that associates Cdc42 with the apical cytoskeleton and thus allows for activation of polarized secretion in gastric parietal cells.

* These authors contributed equally to this work.

§ Corresponding author. E-mail address: xbyao{at}uclink4.berkeley.edu.

Molecular Biology of the Cell
Vol. 14, 1097-1108, March 2003
Copyright © 2003 by The American Society for Cell Biology


This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
E. N. Rittmeyer, S. Daniel, S.-C. Hsu, and M. A. Osman
A dual role for IQGAP1 in regulating exocytosis
J. Cell Sci., February 1, 2008; 121(3): 391 - 403.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
S. Wang, T. Watanabe, J. Noritake, M. Fukata, T. Yoshimura, N. Itoh, T. Harada, M. Nakagawa, Y. Matsuura, N. Arimura, et al.
IQGAP3, a novel effector of Rac1 and Cdc42, regulates neurite outgrowth
J. Cell Sci., February 15, 2007; 120(4): 567 - 577.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
L. B. Bensenor, H.-M. Kan, N. Wang, H. Wallrabe, L. A. Davidson, Y. Cai, D. A. Schafer, and G. S. Bloom
IQGAP1 regulates cell motility by linking growth factor signaling to actin assembly
J. Cell Sci., February 15, 2007; 120(4): 658 - 669.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
C. S. Chew, C. T. Okamoto, X. Chen, and R. Thomas
Drebrin E2 is differentially expressed and phosphorylated in parietal cells in the gastric mucosa
Am J Physiol Gastrointest Liver Physiol, August 1, 2005; 289(2): G320 - G331.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
X. Cao, X. Ding, Z. Guo, R. Zhou, F. Wang, F. Long, F. Wu, F. Bi, Q. Wang, D. Fan, et al.
PALS1 Specifies the Localization of Ezrin to the Apical Membrane of Gastric Parietal Cells
J. Biol. Chem., April 8, 2005; 280(14): 13584 - 13592.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
C. S. Chew, C. T. Okamoto, X. Chen, and H. Y. Qin
IQGAPs are differentially expressed and regulated in polarized gastric epithelial cells
Am J Physiol Gastrointest Liver Physiol, February 1, 2005; 288(2): G376 - G387.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Grohmanova, D. Schlaepfer, D. Hess, P. Gutierrez, M. Beck, and R. Kroschewski
Phosphorylation of IQGAP1 Modulates Its Binding to Cdc42, Revealing a New Type of Rho-GTPase Regulator
J. Biol. Chem., November 19, 2004; 279(47): 48495 - 48504.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. Zhou, X. Cao, C. Watson, Y. Miao, Z. Guo, J. G. Forte, and X. Yao
Characterization of Protein Kinase A-mediated Phosphorylation of Ezrin in Gastric Parietal Cell Activation
J. Biol. Chem., September 12, 2003; 278(37): 35651 - 35659.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]