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Vol. 14, Issue 3, 1149-1157, March 2003
*Department of Medicine, Atherosclerosis Research
Unit, and ¶Department of Molecular Medicine,
Karolinska Institutet, Karolinska Hospital, S-171 76 Stockholm, Sweden;
§Department of Cell and Molecular Biology, and
#Division of Pulmonary and Critical Care Medicine,
Northwestern University Medical School, Chicago, Illinois 60611;
Dopamine (DA) increases Na+,K+-ATPase
activity in lung alveolar epithelial cells. This effect is associated
with an increase in Na+,K+-ATPase molecules
within the plasma membrane (Ridge et al., 2002). Analysis of Na+,K+-ATPase motion was performed
in real-time in alveolar cells stably expressing
Na+,K+-ATPase molecules carrying a fluorescent
tag (green fluorescent protein) in the College of Pharmacy, University of Houston,
Houston, Texas 77204; and @Laboratory of Cell Motility,
A.N. Belozersky Institute, Moscow State University, Moscow, Russia
-subunit. The data
demonstrate a distinct (random walk) pattern of basal movement of
Na+,K+-ATPase-containing vesicles in
nontreated cells. DA increased the directional movement (by 3.5 fold)
of the vesicles and an increase in their velocity (by 25%) that
consequently promoted the incorporation of vesicles into the plasma
membrane. The movement of
Na+,K+-ATPase-containing vesicles and
incorporation into the plasma membrane were microtubule dependent, and
disruption of this network perturbed vesicle motion toward the plasma
membrane and prevented the increase in the
Na+,K+-ATPase activity induced by DA. Thus,
recruitment of new Na+,K+-ATPase molecules into
the plasma membrane appears to be a major mechanism by which dopamine
increases total cell Na+,K+-ATPase activity.
Corresponding author. E-mail address:
aleber{at}mbox.ki.se.
Online version of this article contains video
material. Online version is available at www.molbiolcell.org.
Both authors contributed equally to this work.
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