![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Vol. 14, Issue 3, 1240-1254, March 2003
¶
*Department of Membrane Enzymology, Center for
Biomembranes and Lipid Enzymology, Utrecht University, 3584 CH Utrecht,
The Netherlands; Plasma membranes in eukaryotic cells display asymmetric lipid
distributions with aminophospholipids concentrated in the inner and
sphingolipids in the outer leaflet. This asymmetry is maintained by
ATP-driven lipid transporters whose identities are unknown. The yeast
plasma membrane contains two P-type ATPases, Dnf1p and Dnf2p, with
structural similarity to ATPase II, a candidate aminophospholipid translocase from bovine chromaffin granules. Loss of Dnf1p and Dnf2p
virtually abolished ATP-dependent transport of NBD-labeled phosphatidylethanolamine, phosphatidylserine, and phosphatidylcholine from the outer to the inner plasma membrane leaflet, leaving transport of sphingolipid analogs unaffected. Labeling with trinitrobenzene sulfonic acid revealed that the amount of phosphatidylethanolamine exposed on the surface of Department of Cell Biology and
Histology, Academic Medical Center, 1105 AZ Amsterdam, The Netherlands;
Department of Biochemistry, University of
Geneva, Geneva CH-1211, Switzerland; and
§Institut de Biologie Physico-chimique, 75005 Paris, France
dnf1dnf2 cells increased
twofold relative to wild-type cells. Phosphatidylethanolamine exposure
by dnf1dnf2 cells further increased upon removal
of Drs2p, an ATPase II homolog in the yeast Golgi. These changes in
lipid topology were accompanied by a cold-sensitive defect in the
uptake of markers for bulk-phase and receptor-mediated
endocytosis. Our findings demonstrate a requirement for Dnf1p and Dnf2p
in lipid translocation across the yeast plasma membrane. Moreover, it
appears that Dnf1p, Dnf2p and Drs2p each help regulate the transbilayer
lipid arrangement in the plasma membrane, and that this regulation is
critical for budding endocytic vesicles.
This article has been cited by other articles:
|
|
 |
|
  G. Lenoir, P. Williamson, C. F. Puts, and J. C. M. Holthuis Cdc50p Plays a Vital Role in the ATPase Reaction Cycle of the Putative Aminophospholipid Transporter Drs2p J. Biol. Chem., July 3, 2009; 284(27): 17956 - 17967. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Stapelbroek, T. A. Peters, D. H. A. van Beurden, J. H. A. J. Curfs, A. Joosten, A. J. Beynon, B. M. van Leeuwen, L. M. van der Velden, L. Bull, R. P. Oude Elferink, et al. ATP8B1 is essential for maintaining normal hearing PNAS, June 16, 2009; 106(24): 9709 - 9714. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B.-P. Muthusamy, S. Raychaudhuri, P. Natarajan, F. Abe, K. Liu, W. A. Prinz, and T. R. Graham Control of Protein and Sterol Trafficking by Antagonistic Activities of a Type IV P-type ATPase and Oxysterol Binding Protein Homologue Mol. Biol. Cell, June 15, 2009; 20(12): 2920 - 2931. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. C. Stevens, L. Malone, and J. W. Nichols The Putative Aminophospholipid Translocases, DNF1 and DNF2, Are Not Required for 7-Nitrobenz-2-oxa-1,3-diazol-4-yl-phosphatidylserine Flip across the Plasma Membrane of Saccharomyces cerevisiae J. Biol. Chem., December 12, 2008; 283(50): 35060 - 35069. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Gulshan, J. A. Schmidt, P. Shahi, and W. S. Moye-Rowley Evidence for the Bifunctional Nature of Mitochondrial Phosphatidylserine Decarboxylase: Role in Pdr3-Dependent Retrograde Regulation of PDR5 Expression Mol. Cell. Biol., October 1, 2008; 28(19): 5851 - 5864. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Liu, K. Surendhran, S. F. Nothwehr, and T. R. Graham P4-ATPase Requirement for AP-1/Clathrin Function in Protein Transport from the trans-Golgi Network and Early Endosomes Mol. Biol. Cell, August 1, 2008; 19(8): 3526 - 3535. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Ikeda, A. Kihara, A. Denpoh, and Y. Igarashi The Rim101 Pathway Is Involved in Rsb1 Expression Induced by Altered Lipid Asymmetry Mol. Biol. Cell, May 1, 2008; 19(5): 1922 - 1931. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Nakano, T. Yamamoto, T. Kishimoto, T. Noji, and K. Tanaka Protein Kinases Fpk1p and Fpk2p are Novel Regulators of Phospholipid Asymmetry Mol. Biol. Cell, April 1, 2008; 19(4): 1783 - 1797. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. R. Poulsen, R. L. Lopez-Marques, S. C. McDowell, J. Okkeri, D. Licht, A. Schulz, T. Pomorski, J. F. Harper, and M. G. Palmgren The Arabidopsis P4-ATPase ALA3 Localizes to the Golgi and Requires a {beta}-Subunit to Function in Lipid Translocation and Secretory Vesicle Formation PLANT CELL, March 1, 2008; 20(3): 658 - 676. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Fei, G. Alfaro, B.-P. Muthusamy, Z. Klaassen, T. R. Graham, H. Yang, and C. T. Beh Genome-Wide Analysis of Sterol-Lipid Storage and Trafficking in Saccharomyces cerevisiae Eukaryot. Cell, February 1, 2008; 7(2): 401 - 414. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. R. Riekhof, J. Wu, M. A. Gijon, S. Zarini, R. C. Murphy, and D. R. Voelker Lysophosphatidylcholine Metabolism in Saccharomyces cerevisiae: THE ROLE OF P-TYPE ATPases IN TRANSPORT AND A BROAD SPECIFICITY ACYLTRANSFERASE IN ACYLATION J. Biol. Chem., December 21, 2007; 282(51): 36853 - 36861. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Schuller, Y. M. Mamnun, H. Wolfger, N. Rockwell, J. Thorner, and K. Kuchler Membrane-active Compounds Activate the Transcription Factors Pdr1 and Pdr3 Connecting Pleiotropic Drug Resistance and Membrane Lipid Homeostasis in Saccharomyces cerevisiae Mol. Biol. Cell, December 1, 2007; 18(12): 4932 - 4944. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Szul, R. Grabski, S. Lyons, Y. Morohashi, S. Shestopal, M. Lowe, and E. Sztul Dissecting the role of the ARF guanine nucleotide exchange factor GBF1 in Golgi biogenesis and protein trafficking J. Cell Sci., November 15, 2007; 120(22): 3929 - 3940. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Gulshan and W. S. Moye-Rowley Multidrug Resistance in Fungi Eukaryot. Cell, November 1, 2007; 6(11): 1933 - 1942. [Full Text] [PDF]
|
|
|
|
 |
|
 R. A. Schlegel and P. Williamson P.S. to PS (Phosphatidylserine) Pertinent Proteins in Apoptotic Cell Clearance Sci. Signal., October 16, 2007; 2007(408): pe57 - pe57. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. C. Stevens and J. W. Nichols The Proton Electrochemical Gradient across the Plasma Membrane of Yeast Is Necessary for Phospholipid Flip J. Biol. Chem., June 15, 2007; 282(24): 17563 - 17567. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Liu, Z. Hua, J. A. Nepute, and T. R. Graham Yeast P4-ATPases Drs2p and Dnf1p Are Essential Cargos of the NPFXD/Sla1p Endocytic Pathway Mol. Biol. Cell, February 1, 2007; 18(2): 487 - 500. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. L. Daleke Phospholipid Flippases J. Biol. Chem., January 12, 2007; 282(2): 821 - 825. [Full Text] [PDF]
|
|
|
|
|
|
N. Furuta, K. Fujimura-Kamada, K. Saito, T. Yamamoto, and K. Tanaka Endocytic Recycling in Yeast Is Regulated by Putative Phospholipid Translocases and the Ypt31p/32p-Rcy1p Pathway Mol. Biol. Cell, January 1, 2007; 18(1): 295 - 312. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. R. Riekhof and D. R. Voelker Uptake and Utilization of Lyso-phosphatidylethanolamine by Saccharomyces cerevisiae J. Biol. Chem., December 1, 2006; 281(48): 36588 - 36596. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. J. Perez-Victoria, M. P. Sanchez-Canete, S. Castanys, and F. Gamarro Phospholipid Translocation and Miltefosine Potency Require Both L. donovani Miltefosine Transporter and the New Protein LdRos3 in Leishmania Parasites J. Biol. Chem., August 18, 2006; 281(33): 23766 - 23775. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Alder-Baerens, Q. Lisman, L. Luong, T. Pomorski, and J. C.M. Holthuis Loss of P4 ATPases Drs2p and Dnf3p Disrupts Aminophospholipid Transport and Asymmetry in Yeast Post-Golgi Secretory Vesicles Mol. Biol. Cell, April 1, 2006; 17(4): 1632 - 1642. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Elvington, F. Bu, and J. W. Nichols Fluorescent, Acyl Chain-labeled Phosphatidylcholine Analogs Reveal Novel Transport Pathways across the Plasma Membrane of Yeast J. Biol. Chem., December 9, 2005; 280(49): 40957 - 40964. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Alder-Baerens, P. Muller, A. Pohl, T. Korte, Y. Hamon, G. Chimini, T. Pomorski, and A. Herrmann Headgroup-specific Exposure of Phospholipids in ABCA1-expressing Cells J. Biol. Chem., July 15, 2005; 280(28): 26321 - 26329. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S W C van Mil, R H J Houwen, and L W J Klomp Genetics of familial intrahepatic cholestasis syndromes J. Med. Genet., June 1, 2005; 42(6): 449 - 463. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Kihara and Y. Igarashi Cross Talk between Sphingolipids and Glycerophospholipids in the Establishment of Plasma Membrane Asymmetry Mol. Biol. Cell, November 1, 2004; 15(11): 4949 - 4959. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. T. Doerrler, H. S. Gibbons, and C. R. H. Raetz MsbA-dependent Translocation of Lipids across the Inner Membrane of Escherichia coli J. Biol. Chem., October 22, 2004; 279(43): 45102 - 45109. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Iwamoto, S. Kobayashi, R. Fukuda, M. Umeda, T. Kobayashi, and A. Ohta Local exposure of phosphatidylethanolamine on the yeast plasma membrane is implicated in cell polarity Genes Cells, October 1, 2004; 9(10): 891 - 903. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Wicky, H. Schwarz, and B. Singer-Kruger Molecular Interactions of Yeast Neo1p, an Essential Member of the Drs2 Family of Aminophospholipid Translocases, and Its Role in Membrane Trafficking within the Endomembrane System Mol. Cell. Biol., September 1, 2004; 24(17): 7402 - 7418. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Kalin, J. Fernandes, S. Hrafnsdottir, and G. van Meer Natural Phosphatidylcholine Is Actively Translocated across the Plasma Membrane to the Surface of Mammalian Cells J. Biol. Chem., August 6, 2004; 279(32): 33228 - 33236. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Natarajan, J. Wang, Z. Hua, and T. R. Graham Drs2p-coupled aminophospholipid translocase activity in yeast Golgi membranes and relationship to in vivo function PNAS, July 20, 2004; 101(29): 10614 - 10619. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Saito, K. Fujimura-Kamada, N. Furuta, U. Kato, M. Umeda, and K. Tanaka Cdc50p, a Protein Required for Polarized Growth, Associates with the Drs2p P-Type ATPase Implicated in Phospholipid Translocation in Saccharomyces cerevisiae Mol. Biol. Cell, July 1, 2004; 15(7): 3418 - 3432. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Pomorski, J. C. M. Holthuis, A. Herrmann, and G. van Meer Tracking down lipid flippases and their biological functions J. Cell Sci., February 22, 2004; 117(6): 805 - 813. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Chantalat, S.-K. Park, Z. Hua, K. Liu, R. Gobin, A. Peyroche, A. Rambourg, T. R. Graham, and C. L. Jackson The Arf activator Gea2p and the P-type ATPase Drs2p interact at the Golgi in Saccharomyces cerevisiae J. Cell Sci., February 15, 2004; 117(5): 711 - 722. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. J. Perez-Victoria, F. Gamarro, M. Ouellette, and S. Castanys Functional Cloning of the Miltefosine Transporter: A NOVEL P-TYPE PHOSPHOLIPID TRANSLOCASE FROM LEISHMANIA INVOLVED IN DRUG RESISTANCE J. Biol. Chem., December 12, 2003; 278(50): 49965 - 49971. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Hua and T. R. Graham Requirement for Neo1p in Retrograde Transport from the Golgi Complex to the Endoplasmic Reticulum Mol. Biol. Cell, December 1, 2003; 14(12): 4971 - 4983. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. K. Hanson, L. Malone, J. L. Birchmore, and J. W. Nichols Lem3p Is Essential for the Uptake and Potency of Alkylphosphocholine Drugs, Edelfosine and Miltefosine J. Biol. Chem., September 19, 2003; 278(38): 36041 - 36050. [Abstract] [Full Text] [PDF]
|
|
