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Vol. 14, Issue 3, 987-1001, March 2003
Department of Molecular Biology and Genetics, Graduate
Program in Cellular and Molecular Medicine, The Johns Hopkins
University School of Medicine, Baltimore, Maryland 21205
Telomerase-deficient Saccharomyces cerevisiae cells
show a progressive decrease in telomere length. When grown for several days in log phase, the tlc1
cells initially display
wild-type growth kinetics with subsequent loss of growth potential
after which survivors are generated via RAD52-dependent
homologous recombination. We found that chromosome loss in these
telomerase-deficient cells only increased after a significant decline
in growth potential of the culture. At earlier stages of growth, as the
telomerase-deficient cells began to show loss of growth potential, the
cells arrested in G2/M and showed RNR3 induction and
Rad53p phosphorylation. These responses were dependent on
RAD24 and MEC1, suggesting that short
telomeres are recognized as DNA damage and signal G2/M arrest.
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