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Vol. 14, Issue 4, 1664-1676, April 2003
Departments of Biochemistry and Genetics, Dartmouth
Medical School, Hanover, New Hampshire 03755
The yeast DEAD-box protein Dbp5p/Rat8p is an essential factor for
mRNA export and shuttles between the nucleus and the cytoplasm. It is
concentrated at the cytoplasmic fibrils of the nuclear pore complex where it interacts with several nucleoporins. On the
basis of this localization, it has been suggested that it might
participate in a terminal step of RNA export, the release from the mRNA
of proteins that accompany the mRNA during translocation through nuclear pores. In this report, we present evidence linking Dbp5p to
transcription. Two different screens identified genetic interactions between DBP5 and genes involved in early transcription
events, initiation and promoter clearance. Mutations of transcription proteins expected to impair transcription act as suppressors of dbp5 mutants, whereas those that may act to increase
transcription are synthetically lethal with dbp5
mutations. We also show that growth and mRNA export in
dbp5 mutant strains are dependent on the
carboxy-terminal domain of the RNA pol II largest subunit. Finally, we
show that Dbp5p associates physically with components of transcription
factor IIH. Because these interactions affect not only growth but also
mRNA export, they are likely to reflect a functional relationship
between Dbp5p and the transcription machinery. Together, our results
suggest a nuclear role for Dbp5 during the early steps of transcription.
Corresponding author. E-mail address:
charles.cole{at}dartmouth.edu.
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