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Vol. 14, Issue 5, 1769-1779, May 2003
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||School of Biological Sciences, University of Manchester, Manchester, M13 9PT England
Submitted February 22, 2002;
Revised January 8, 2003;
Accepted January 30, 2003
Monitoring Editor: Paul Matsudaira
In trypanosomes, the large mitochondrial genome within the kinetoplast is physically connected to the flagellar basal bodies and is segregated by them during cell growth. The structural linkage enabling these phenomena is unknown. We have developed novel extraction/fixation protocols to characterize the links involved in kinetoplast-flagellum attachment and segregation. We show that three specific components comprise a structure that we have termed the tripartite attachment complex (TAC). The TAC involves a set of filaments linking the basal bodies to a zone of differentiated outer and inner mitochondrial membranes and a further set of intramitochondrial filaments linking the inner face of the differentiated membrane zone to the kinetoplast. The TAC and flagellum-kinetoplast DNA connections are sustained throughout the cell cycle and are replicated and remodeled during the periodic kinetoplast DNA S phase. This understanding of the high-order trans-membrane linkage provides an explanation for the spatial position of the trypanosome mitochondrial genome and its mechanism of segregation. Moreover, the architecture of the TAC suggests that it may also function in providing a structural and vectorial role during replication of this catenated mass of mitochondrial DNA. We suggest that this complex may represent an extreme form of a more generally occurring mitochondrion/cytoskeleton interaction.
Abbreviations used: kDNA, kinetoplast DNA; TAC, tripartite attachment complex.
* These authors share first authorship on this article.
Present address: Department of Biological Sciences, Federal University of
Technology, Bosso Rd., P.M.B. 65, Minna, Nigeria
Present address: Laboratoire de Parasitologie Molelulaire, Unité
Mixte Recherche-Centre National de la Recherche Scientifique 5016,
Université Bordeaux 2, 146 rue Léo Saignat, 33076 Bordeaux
Cedex, France
Present address: Sir William Dunn School of Pathology, University of
Oxford, South Parks Rd., Oxford OX1 3RE, United Kingdom.
|| Corresponding author. E-mail address: keith.gull{at}pathology.ox.ac.uk.
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