QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

Vol. 14, Issue 5, 1818-1834, May 2003

This Article Full Text Full Text (PDF) All Versions of this Article: E02-11-0709v1 14/5/1818    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Gavet, O. Articles by Bornens, M. Search for Related Content PubMed PubMed Citation Articles by Gavet, O. Articles by Bornens, M.

Centrin4p, a Novel Mammalian Centrin Specifically Expressed in Ciliated Cells

Olivier Gavet ^* , Chantale Alvarez , Patricia Gaspar , and Michel Bornens ^* 

^* Institut Curie, Section Recherche, Unité Mixte Recherche 144 du Centre National de la Recherche Scientifique, 75248 Paris, France; Institut National de la Santé et de la Recherche Médicale U106, Bat. Pédiatrie, Hopital Salpêtrière, 75651 Paris, France

Submitted November 5, 2002; Revised December 13, 2002; Accepted January 23, 2003
Monitoring Editor: Tim Stearns

Centriole assembly plays an important role in centrosome duplication during the cell cycle and is a prerequisite for cilia formation during the differentiation of ciliated cells. In spite of numerous investigations, the molecular machinery that governs centriole/basal body formation remains enigmatic. Recent reports suggest that the ubiquitously expressed mammalian centrins, centrin2p and centrin3p, could be involved in the centriole duplication process. To better understand the specific functions of these proteins, we performed a systematic search for novel mammalian centrins. We isolated a cDNA and the corresponding gene coding for a novel murine centrin, centrin4p, which is more closely related to centrin2p. Like centrin2p, centrin4p accumulates to centrioles and procentrioles when ectopically expressed in HeLa cells. However, centrin4p possesses two splice variants that do not localize to centrioles, suggesting a posttranscriptional regulation mechanism. We also observed that centrin4p does not share the same centriolar targeting properties with centrin2p and 3p, indicating that these proteins could recognize different centriolar partners. Centrin4 mRNA possesses a restricted expression profile and is only detected in brain, kidney, lung, and ovary. In brain, centrin4p is exclusively expressed in ependymal and choroidal ciliated cells where it is localized to basal bodies. Together, our present data suggest that centrin4p could be more specifically involved in basal bodies assembly or in a subsequent step of ciliogenesis.

Present address: Laboratoire de Biologie Cellulaire du Développement, Unité Mixte Recherche 7622, Centre National de la Recherche Scientifique, Université Pierre et Marie Curie, 9 quai Saint-Bernard, 72252 Paris Cedex 05, France.

Corresponding author. E-mail address: mbornens{at}curie.fr.

This article has been cited by other articles:

				S. Mana-Capelli, R. Graf, and D. A. Larochelle Dictyostelium discoideum CenB Is a Bona Fide Centrin Essential for Nuclear Architecture and Centrosome Stability Eukaryot. Cell, August 1, 2009; 8(8): 1106 - 1117. [Abstract] [Full Text] [PDF]

				A. Kubo, A. Yuba-Kubo, S. Tsukita, S. Tsukita, and M. Amagai Sentan: A Novel Specific Component of the Apical Structure of Vertebrate Motile Cilia Mol. Biol. Cell, December 1, 2008; 19(12): 5338 - 5346. [Abstract] [Full Text] [PDF]

				B. Mahajan, A. Selvapandiyan, N. J. Gerald, V. Majam, H. Zheng, T. Wickramarachchi, J. Tiwari, H. Fujioka, J. K. Moch, N. Kumar, et al. Centrins, Cell Cycle Regulation Proteins in Human Malaria Parasite Plasmodium falciparum J. Biol. Chem., November 14, 2008; 283(46): 31871 - 31883. [Abstract] [Full Text] [PDF]

				A. Selvapandiyan, P. Kumar, J. C. Morris, J. L. Salisbury, C. C. Wang, and H. L. Nakhasi Centrin1 Is Required for Organelle Segregation and Cytokinesis in Trypanosoma brucei Mol. Biol. Cell, September 1, 2007; 18(9): 3290 - 3301. [Abstract] [Full Text] [PDF]

				R. Kuriyama, Y. Terada, K. S. Lee, and C. L. C. Wang Centrosome replication in hydroxyurea-arrested CHO cells expressing GFP-tagged centrin2 J. Cell Sci., July 15, 2007; 120(14): 2444 - 2453. [Abstract] [Full Text] [PDF]

				J. R. Thompson, Z. C. Ryan, J. L. Salisbury, and R. Kumar The Structure of the Human Centrin 2-Xeroderma Pigmentosum Group C Protein Complex J. Biol. Chem., July 7, 2006; 281(27): 18746 - 18752. [Abstract] [Full Text] [PDF]

				S. Geimer and M. Melkonian Centrin Scaffold in Chlamydomonas reinhardtii Revealed by Immunoelectron Microscopy Eukaryot. Cell, July 1, 2005; 4(7): 1253 - 1263. [Abstract] [Full Text] [PDF]

				A. Giessl, A. Pulvermuller, P. Trojan, J. H. Park, H.-W. Choe, O. P. Ernst, K. P. Hofmann, and U. Wolfrum Differential Expression and Interaction with the Visual G-protein Transducin of Centrin Isoforms in Mammalian Photoreceptor Cells J. Biol. Chem., December 3, 2004; 279(49): 51472 - 51481. [Abstract] [Full Text] [PDF]

				M. Tourbez, C. Firanescu, A. Yang, L. Unipan, P. Duchambon, Y. Blouquit, and C. T. Craescu Calcium-dependent Self-assembly of Human Centrin 2 J. Biol. Chem., November 12, 2004; 279(46): 47672 - 47680. [Abstract] [Full Text] [PDF]

				A. Selvapandiyan, A. Debrabant, R. Duncan, J. Muller, P. Salotra, G. Sreenivas, J. L. Salisbury, and H. L. Nakhasi Centrin Gene Disruption Impairs Stage-specific Basal Body Duplication and Cell Cycle Progression in Leishmania J. Biol. Chem., June 11, 2004; 279(24): 25703 - 25710. [Abstract] [Full Text] [PDF]

				J. V. Kilmartin Sfi1p has conserved centrin-binding sites and an essential function in budding yeast spindle pole body duplication J. Cell Biol., September 29, 2003; 162(7): 1211 - 1221. [Abstract] [Full Text] [PDF]