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Vol. 14, Issue 5, 1953-1963, May 2003
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Department of Cell Biology and The Center for Blood Research, Harvard Medical School, Boston, Massachusetts 02115
Submitted October 16, 2002;
Revised December 9, 2002;
Accepted January 16, 2003
Monitoring Editor: Vivek Malhotra
Mitochondria undergo cycles of fusion and fission crucial for organelle homeostasis. Fission is regulated partially by recruitment of the large GTPase Dnm1p to the outer mitochondrial membrane. Using three-dimensional time-lapse fluorescence imaging of Saccharomyces cerevisiae cells, we found that Dnm1p-EGFP appears and disappears at "hot spots" along mitochondrial tubes. It forms patches that convert rapidly into different shapes regardless of whether mitochondrial fission ensues or not. Moreover, the thickness of the mitochondrial matrix displays frequent temporal fluctuations apparently unrelated to fission or to recruitment of Dnm1p-EGFP. These results suggest that mitochondrial fission requires coordination of at least two distinct processes.
Online version of this article contains video material for some figures.
Online version available at
www.molbiolcell.org.
* These authors contributed equally to this work.
Corresponding author. E-mail address:
kirchhausen{at}crystal.harvard.edu.
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