QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

Vol. 14, Issue 5, 2151-2162, May 2003

This Article Full Text Full Text (PDF) All Versions of this Article: E02-07-0451v1 14/5/2151    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by L'honore, A. Articles by Fernandez, A. Search for Related Content PubMed PubMed Citation Articles by L'honore, A. Articles by Fernandez, A.

MyoD Distal Regulatory Region Contains an SRF Binding CArG Element Required for MyoD Expression in Skeletal Myoblasts and during Muscle Regeneration

Aurore L'honore, Ned J. Lamb, Marie Vandromme  , Patric Turowski , Gilles Carnac , and Anne Fernandez 

Cell Biology Unit, Institut de Génétique Humaine, 34396 Montpellier cedex 05, France

Submitted July 31, 2002; Revised December 20, 2002; Accepted January 7, 2003
Monitoring Editor: Keith Yamamoto

We show here that the distal regulatory region (DRR) of the mouse and human MyoD gene contains a conserved SRF binding CArG-like element. In electrophoretic mobility shift assays with myoblast nuclear extracts, this CArG sequence, although slightly divergent, bound two complexes containing, respectively, the transcription factor YY1 and SRF associated with the acetyltransferase CBP and members of C/EBP family. A single nucleotide mutation in the MyoD-CArG element suppressed binding of both SRF and YY1 complexes and abolished DRR enhancer activity in stably transfected myoblasts. This MyoD-CArG sequence is active in modulating endogeneous MyoD gene expression because microinjection of oligonucleotides corresponding to the MyoD-CArG sequence specifically and rapidly suppressed MyoD expression in myoblasts. In vivo, the expression of a transgenic construct comprising a minimal MyoD promoter fused to the DRR and -galactosidase was induced with the same kinetics as MyoD during mouse muscle regeneration. In contrast induction of this reporter was no longer seen in regenerating muscle from transgenic mice carrying a mutated DRR-CArG. These results show that an SRF binding CArG element present in MyoD gene DRR is involved in the control of MyoD gene expression in skeletal myoblasts and in mature muscle satellite cell activation during muscle regeneration.

Present address: Ecole Normale Supérieure de Lyon, Laboratoire de Biologie Moléculaire et Cellulaire, 46, allée d'Italie, 69364 Lyon cedex 07, France

Present address: Endothelial and Epithelial Cell Biology, Division of Cell Biology, Institute of Ophtalmology, University College London, Bath Street, London ECIV 9EL, England.

Both authors contributed equally to this work.

Corresponding author. E-mail address: af{at}acrux.igh.cnrs.fr.

This article has been cited by other articles:

				J.-R. Kim, H. J. Kee, J.-Y. Kim, H. Joung, K.-I. Nam, G. H. Eom, N. Choe, H.-S. Kim, J. C. Kim, H. Kook, et al. Enhancer of Polycomb1 Acts on Serum Response Factor to Regulate Skeletal Muscle Differentiation J. Biol. Chem., June 12, 2009; 284(24): 16308 - 16316. [Abstract] [Full Text] [PDF]

				K. Iwasaki, K. Hayashi, T. Fujioka, and K. Sobue Rho/Rho-associated Kinase Signal Regulates Myogenic Differentiation via Myocardin-related Transcription Factor-A/Smad-dependent Transcription of the Id3 Gene J. Biol. Chem., July 25, 2008; 283(30): 21230 - 21241. [Abstract] [Full Text] [PDF]

				S. D. Gopinath, S. Narumiya, and J. Dhawan The RhoA effector mDiaphanous regulates MyoD expression and cell cycle progression via SRF-dependent and SRF-independent pathways J. Cell Sci., September 1, 2007; 120(17): 3086 - 3098. [Abstract] [Full Text] [PDF]

				A. L'honore, V. Rana, N. Arsic, C. Franckhauser, N. J. Lamb, and A. Fernandez Identification of a New Hybrid Serum Response Factor and Myocyte Enhancer Factor 2-binding Element in MyoD Enhancer Required for MyoD Expression during Myogenesis Mol. Biol. Cell, June 1, 2007; 18(6): 1992 - 2001. [Abstract] [Full Text] [PDF]

				P. Pomies, M. Pashmforoush, C. Vegezzi, K. R. Chien, C. Auffray, and M. C. Beckerle The Cytoskeleton-associated PDZ-LIM Protein, ALP, Acts on Serum Response Factor Activity to Regulate Muscle Differentiation Mol. Biol. Cell, May 1, 2007; 18(5): 1723 - 1733. [Abstract] [Full Text] [PDF]

				C. Charvet, C. Houbron, A. Parlakian, J. Giordani, C. Lahoute, A. Bertrand, A. Sotiropoulos, L. Renou, A. Schmitt, J. Melki, et al. New Role for Serum Response Factor in Postnatal Skeletal Muscle Growth and Regeneration via the Interleukin 4 and Insulin-Like Growth Factor 1 Pathways. Mol. Cell. Biol., September 1, 2006; 26(17): 6664 - 6674. [Abstract] [Full Text] [PDF]

				B. A. Bryan, D. C. Mitchell, L. Zhao, W. Ma, L. J. Stafford, B.-B. Teng, and M. Liu Modulation of Muscle Regeneration, Myogenesis, and Adipogenesis by the Rho Family Guanine Nucleotide Exchange Factor GEFT Mol. Cell. Biol., December 15, 2005; 25(24): 11089 - 11101. [Abstract] [Full Text] [PDF]

				C. Rene, M. Taulan, F. Iral, J. Doudement, A. L'Honore, C. Gerbon, J. Demaille, M. Claustres, and M.-C. Romey Binding of serum response factor to cystic fibrosis transmembrane conductance regulator CArG-like elements, as a new potential CFTR transcriptional regulation pathway Nucleic Acids Res., September 16, 2005; 33(16): 5271 - 5290. [Abstract] [Full Text] [PDF]

				G. Caretti, M. Di Padova, B. Micales, G. E. Lyons, and V. Sartorelli The Polycomb Ezh2 methyltransferase regulates muscle gene expression and skeletal muscle differentiation Genes & Dev., November 1, 2004; 18(21): 2627 - 2638. [Abstract] [Full Text] [PDF]

				A. Rochat, A. Fernandez, M. Vandromme, J.-P. Moles, T. Bouschet, G. Carnac, and N. J. C. Lamb Insulin and Wnt1 Pathways Cooperate to Induce Reserve Cell Activation in Differentiation and Myotube Hypertrophy Mol. Biol. Cell, October 1, 2004; 15(10): 4544 - 4555. [Abstract] [Full Text] [PDF]