![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 14, Issue 6, 2237-2249, June 2003
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
* Division of Molecular Interaction, Institute for Genetic Medicine, Hokkaido
University Graduate School of Medicine, Hokkaido 060-0815, Japan;
Department of Molecular Biology and Biochemistry, Osaka University Graduate
School of Medicine, Osaka 565-0871, Japan; and
Department of General Surgery, Hokkaido University Graduate School of
Medicine, Hokkaido 060-8638, Japan
Submitted September 27, 2002;
Revised January 10, 2003;
Accepted January 30, 2003
Monitoring Editor: David Drubin
She4p/Dim1p, a member of the UNC-45/CRO1/She4p (UCS) domain-containing protein family, is required for endocytosis, polarization of actin cytoskeleton, and polarization of ASH1 mRNA in Saccharomyces cerevisiae. We show herein that She4p/Dim1p is involved in endocytosis and actin polarization through interactions with the type I myosins Myo3p and Myo5p. Two-hybrid and biochemical experiments showed that She4p/Dim1p interacts with the motor domain of Myo3/5p through its UCS domain. She4p/Dim1p was required for Myo5p localization to cortical patch-like structures. Using random mutagenesis of the motor region of MYO5, we identified four independent dominant point mutations that suppress the temperature-sensitive growth phenotype of the she4/dim1 null mutant. All of the amino acid substitutions caused by these mutations, V164I, N168I, N209S, and K377M, could suppress the defects of endocytosis and actin polarization of the she4/dim1 mutant as well. She4p/Dim1p also showed two-hybrid interactions with the motor domain of a type II myosin Myo1p and type V myosins Myo2p and Myo4p, and was required for proper localization of Myo4p, which regulates polarization of ASH1 mRNA. Our results suggest that She4p/Dim1p is required for structural integrity or regulation of the motor domain of unconventional myosins.
Corresponding author. E-mail address:
k-tanaka{at}med.hokudai.ac.jp.
This article has been cited by other articles:
|
|
 |
|
  M. Lord, T. E. Sladewski, and T. D. Pollard Yeast UCS proteins promote actomyosin interactions and limit myosin turnover in cells PNAS, June 10, 2008; 105(23): 8014 - 8019. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Etard, U. Roostalu, and U. Strahle Shuttling of the chaperones Unc45b and Hsp90a between the A band and the Z line of the myofibril J. Cell Biol., March 24, 2008; 180(6): 1163 - 1175. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. L. Barker, L. Lee, B. D. Pierce, L. Maldonado-Baez, D. G. Drubin, and B. Wendland Interaction of the Endocytic Scaffold Protein Pan1 with the Type I Myosins Contributes to the Late Stages of Endocytosis Mol. Biol. Cell, August 1, 2007; 18(8): 2893 - 2903. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Furuta, K. Fujimura-Kamada, K. Saito, T. Yamamoto, and K. Tanaka Endocytic Recycling in Yeast Is Regulated by Putative Phospholipid Translocases and the Ypt31p/32p-Rcy1p Pathway Mol. Biol. Cell, January 1, 2007; 18(1): 295 - 312. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Kishimoto, T. Yamamoto, and K. Tanaka Defects in Structural Integrity of Ergosterol and the Cdc50p-Drs2p Putative Phospholipid Translocase Cause Accumulation of Endocytic Membranes, onto Which Actin Patches Are Assembled in Yeast Mol. Biol. Cell, December 1, 2005; 16(12): 5592 - 5609. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Lord, E. Laves, and T. D. Pollard Cytokinesis Depends on the Motor Domains of Myosin-II in Fission Yeast but Not in Budding Yeast Mol. Biol. Cell, November 1, 2005; 16(11): 5346 - 5355. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Kadota, T. Yamamoto, S. Yoshiuchi, E. Bi, and K. Tanaka Septin Ring Assembly Requires Concerted Action of Polarisome Components, a PAK Kinase Cla4p, and the Actin Cytoskeleton in Saccharomyces cerevisiae Mol. Biol. Cell, December 1, 2004; 15(12): 5329 - 5345. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Lord and T. D. Pollard UCS protein Rng3p activates actin filament gliding by fission yeast myosin-II J. Cell Biol., October 25, 2004; 167(2): 315 - 325. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Kachur, W. Ao, J. Berger, and D. Pilgrim Maternal UNC-45 is involved in cytokinesis and colocalizes with non-muscle myosin in the early Caenorhabditis elegans embryo J. Cell Sci., October 15, 2004; 117(22): 5313 - 5321. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Aronov and J. E. Gerst Involvement of the Late Secretory Pathway in Actin Regulation and mRNA Transport in Yeast J. Biol. Chem., August 27, 2004; 279(35): 36962 - 36971. [Abstract] [Full Text] [PDF]
|
|
