![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 14, Issue 6, 2277-2291, June 2003
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
* Department of Molecular, Cellular, and Developmental Biology, University of
Colorado, Boulder, Colorado 80309-0347;
Department of Molecular Genetics and Cell Biology, The University of Chicago,
Chicago, Illinois 60637
Submitted October 30, 2003;
Revised January 2, 2003;
Accepted March 4, 2003
Monitoring Editor: Vivek Malhotra
The budding yeast Pichia pastoris contains ordered Golgi stacks next to discrete transitional endoplasmic reticulum (tER) sites, making this organism ideal for structure–function studies of the secretory pathway. Here, we have used P. pastoris to test various models for Golgi trafficking. The experimental approach was to analyze P. pastoris tER-Golgi units by using cryofixed and freeze-substituted cells for electron microscope tomography, immunoelectron microscopy, and serial thin section analysis of entire cells. We find that tER sites and the adjacent Golgi stacks are enclosed in a ribosome-excluding "matrix." Each stack contains three to four cisternae, which can be classified as cis, medial, trans, or trans-Golgi network (TGN). No membrane continuities between compartments were detected. This work provides three major new insights. First, two types of transport vesicles accumulate at the tER-Golgi interface. Morphological analysis indicates that the center of the tER-Golgi interface contains COPII vesicles, whereas the periphery contains COPI vesicles. Second, fenestrae are absent from cis cisternae, but are present in medial through TGN cisternae. The number and distribution of the fenestrae suggest that they form at the edges of the medial cisternae and then migrate inward. Third, intact TGN cisternae apparently peel off from the Golgi stacks and persist for some time in the cytosol, and these "free-floating" TGN cisternae produce clathrin-coated vesicles. These observations are most readily explained by assuming that Golgi cisternae form at the cis face of the stack, progressively mature, and ultimately dissociate from the trans face of the stack.
Online version of this article contains video material for some figures.
Online version available at
www.molbiolcell.org.
S. Mogelsuang and N. Gomez-Ospina contributed equally to this work.
Corresponding author. E-mail address:
soren.mogelsvang{at}uchsc.edu.
This article has been cited by other articles:
|
|
 |
|
  R. Sinka, A. K. Gillingham, V. Kondylis, and S. Munro Golgi coiled-coil proteins contain multiple binding sites for Rab family G proteins J. Cell Biol., November 17, 2008; 183(4): 607 - 615. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Ivan, G. de Voer, D. Xanthakis, K. M. Spoorendonk, V. Kondylis, and C. Rabouille Drosophila Sec16 Mediates the Biogenesis of tER Sites Upstream of Sar1 through an Arginine-Rich Motif Mol. Biol. Cell, October 1, 2008; 19(10): 4352 - 4365. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. A. Staehelin and B.-H. Kang Nanoscale Architecture of Endoplasmic Reticulum Export Sites and of Golgi Membranes as Determined by Electron Tomography Plant Physiology, August 1, 2008; 147(4): 1454 - 1468. [Full Text] [PDF]
|
|
|
|
 |
|
 L. Jackson-Hayes, T. W. Hill, D. M. Loprete, L. M. Fay, B. S. Gordon, S. A. Nkashama, R. K. Patel, and C. V. Sartain Two GDP-mannose transporters contribute to hyphal form and cell wall integrity in Aspergillus nidulans Microbiology, July 1, 2008; 154(7): 2037 - 2047. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Hubbard and S. G. W. Kaminskyj Rapid tip-directed movement of Golgi equivalents in growing Aspergillus nidulans hyphae suggests a mechanism for delivery of growth-related materials Microbiology, May 1, 2008; 154(5): 1544 - 1553. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. J. Haas, M. K. Sliwinski, D. E. Martinez, M. Preuss, K. Ebine, T. Ueda, E. Nielsen, G. Odorizzi, and M. S. Otegui The Arabidopsis AAA ATPase SKD1 Is Involved in Multivesicular Endosome Function and Interacts with Its Positive Regulator LYST-INTERACTING PROTEIN5 PLANT CELL, April 1, 2007; 19(4): 1295 - 1312. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Bhattacharyya and B. S. Glick Two Mammalian Sec16 Homologues Have Nonredundant Functions in Endoplasmic Reticulum (ER) Export and Transitional ER Organization Mol. Biol. Cell, March 1, 2007; 18(3): 839 - 849. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. S. Donohoe, B.-H. Kang, and L. A. Staehelin Identification and characterization of COPIa- and COPIb-type vesicle classes associated with plant and algal Golgi PNAS, January 2, 2007; 104(1): 163 - 168. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Moreau, F. Brandizzi, S. Hanton, L. Chatre, S. Melser, C. Hawes, and B. Satiat-Jeunemaitre The plant ER-Golgi interface: a highly structured and dynamic membrane complex J. Exp. Bot., January 1, 2007; 58(1): 49 - 64. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Kondylis, K. M. Spoorendonk, and C. Rabouille dGRASP Localization and Function in the Early Exocytic Pathway in Drosophila S2 Cells Mol. Biol. Cell, September 1, 2005; 16(9): 4061 - 4072. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-d. Yang, R. Elamawi, J. Bubeck, R. Pepperkok, C. Ritzenthaler, and D. G. Robinson Dynamics of COPII Vesicles and the Golgi Apparatus in Cultured Nicotiana tabacum BY-2 Cells Provides Evidence for Transient Association of Golgi Stacks with Endoplasmic Reticulum Exit Sites PLANT CELL, May 1, 2005; 17(5): 1513 - 1531. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. E. Abazeed, J. M. Blanchette, and R. S. Fuller Cell-free Transport from the trans-Golgi Network to Late Endosome Requires Factors Involved in Formation and Consumption of Clathrin-coated Vesicles J. Biol. Chem., February 11, 2005; 280(6): 4442 - 4450. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. Reinke, P. Kozik, and B. S. Glick Golgi inheritance in small buds of Saccharomyces cerevisiae is linked to endoplasmic reticulum inheritance PNAS, December 28, 2004; 101(52): 18018 - 18023. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-W. Shin and K. Nakayama Guanine Nucleotide-Exchange Factors for Arf GTPases: Their Diverse Functions in Membrane Traffic J. Biochem., December 1, 2004; 136(6): 761 - 767. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Herpers and C. Rabouille mRNA Localization and ER-based Protein Sorting Mechanisms Dictate the Use of Transitional Endoplasmic Reticulum-Golgi Units Involved in Gurken Transport in Drosophila Oocytes Mol. Biol. Cell, December 1, 2004; 15(12): 5306 - 5317. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Kondylis and C. Rabouille A novel role for dp115 in the organization of tER sites in Drosophila J. Cell Biol., July 21, 2003; 162(2): 185 - 198. [Abstract] [Full Text] [PDF]
|
|
