![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 14, Issue 6, 2357-2371, June 2003
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
* SBGM, CEA Saclay, 91191 Gif-sur-Yvette Cedex, France;
UMR CNRS 144, Institut Curie, 75248 Paris Cedex 05, France
Submitted October 30, 2002;
Revised January 23, 2003;
Accepted February 22, 2003
Monitoring Editor: Vivek Malhotra
The Sec7 domain guanine nucleotide exchange factors (GEFs) for the GTPase ARF are highly conserved regulators of membrane dynamics and protein trafficking. The interactions of large ARF GEFs with cellular membranes for localization and/or activation are likely to participate in regulated recruitment of ARF and effectors. However, these interactions remain largely unknown. Here we characterize Gmh1p, the first Golgi transmembrane-domain partner of any of the high-molecular-weight ARF-GEFs. Gmh1p is an evolutionarily conserved protein. We demonstrate molecular interaction between the yeast Gmh1p and the large ARF-GEFs Gea1p and Gea2p. This interaction involves a domain of Gea1p and Gea2p that is conserved in the eukaryotic orthologues of the Gea proteins. A single mutation in a conserved amino acid residue of this domain is sufficient to abrogate the interaction, whereas the overexpression of Gmh1p can compensate in vivo defects caused by mutations in this domain. We show that Gmh1p is an integral membrane protein that localizes to the early Golgi in yeast and in human HeLa cells and cycles through the ER. Hence, we propose that Gmh1p acts as a positive Golgi-membrane partner for Gea function. These results are of general interest given the evolutionary conservation of both ARF-GEFs and the Gmh proteins.
Present address: Cell Biology and Metabolism Branch, NICHD, NIH, Bethesda,
MD 20892-5430.
Corresponding author. E-mail address:
annep{at}Matthieu.saclay.cea.fr.
This article has been cited by other articles:
|
|
 |
|
  Y. Miyazawa, A. Takahashi, A. Kobayashi, T. Kaneyasu, N. Fujii, and H. Takahashi GNOM-Mediated Vesicular Trafficking Plays an Essential Role in Hydrotropism of Arabidopsis Roots Plant Physiology, February 1, 2009; 149(2): 835 - 840. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Chun, Z. Shapovalova, S. Y. Dejgaard, J. F. Presley, and P. Melancon Characterization of Class I and II ADP-Ribosylation Factors (Arfs) in Live Cells: GDP-bound Class II Arfs Associate with the ER-Golgi Intermediate Compartment Independently of GBF1 Mol. Biol. Cell, August 1, 2008; 19(8): 3488 - 3500. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Anders, M. Nielsen, J. Keicher, Y.-D. Stierhof, M. Furutani, M. Tasaka, K. Skriver, and G. Jurgens Membrane Association of the Arabidopsis ARF Exchange Factor GNOM Involves Interaction of Conserved Domains PLANT CELL, January 1, 2008; 20(1): 142 - 151. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Szul, R. Grabski, S. Lyons, Y. Morohashi, S. Shestopal, M. Lowe, and E. Sztul Dissecting the role of the ARF guanine nucleotide exchange factor GBF1 in Golgi biogenesis and protein trafficking J. Cell Sci., November 15, 2007; 120(22): 3929 - 3940. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Monetta, I. Slavin, N. Romero, and C. Alvarez Rab1b Interacts with GBF1 and Modulates both ARF1 Dynamics and COPI Association Mol. Biol. Cell, July 1, 2007; 18(7): 2400 - 2410. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Salamat-Miller, J. Fang, C. W. Seidel, Y. Assenov, M. Albrecht, and C. R. Middaugh A Network-based Analysis of Polyanion-binding Proteins Utilizing Human Protein Arrays J. Biol. Chem., April 6, 2007; 282(14): 10153 - 10163. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. K. Gillingham, J. R. C. Whyte, B. Panic, and S. Munro Mon2, a Relative of Large Arf Exchange Factors, Recruits Dop1 to the Golgi Apparatus J. Biol. Chem., January 27, 2006; 281(4): 2273 - 2280. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-K. Park, L. M. Hartnell, and C. L. Jackson Mutations in a Highly Conserved Region of the Arf1p Activator GEA2 Block Anterograde Golgi Transport but Not COPI Recruitment to Membranes Mol. Biol. Cell, August 1, 2005; 16(8): 3786 - 3799. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T.-K. Niu, A. C. Pfeifer, J. Lippincott-Schwartz, and C. L. Jackson Dynamics of GBF1, a Brefeldin A-Sensitive Arf1 Exchange Factor at the Golgi Mol. Biol. Cell, March 1, 2005; 16(3): 1213 - 1222. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Heinrich and T. A. Rapoport Generation of nonidentical compartments in vesicular transport systems J. Cell Biol., January 17, 2005; 168(2): 271 - 280. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-W. Shin and K. Nakayama Guanine Nucleotide-Exchange Factors for Arf GTPases: Their Diverse Functions in Membrane Traffic J. Biochem., December 1, 2004; 136(6): 761 - 767. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Cox, R. J Mason-Gamer, C. L. Jackson, and N. Segev Phylogenetic Analysis of Sec7-Domain-containing Arf Nucleotide Exchangers Mol. Biol. Cell, April 1, 2004; 15(4): 1487 - 1505. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Chantalat, S.-K. Park, Z. Hua, K. Liu, R. Gobin, A. Peyroche, A. Rambourg, T. R. Graham, and C. L. Jackson The Arf activator Gea2p and the P-type ATPase Drs2p interact at the Golgi in Saccharomyces cerevisiae J. Cell Sci., February 15, 2004; 117(5): 711 - 722. [Abstract] [Full Text] [PDF]
|
|
