QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

Vol. 14, Issue 6, 2436-2446, June 2003

This Article Full Text Full Text (PDF) All Versions of this Article: E02-09-0614v1 14/6/2436    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Keryer, G. Articles by Bornens, M. Search for Related Content PubMed PubMed Citation Articles by Keryer, G. Articles by Bornens, M.

Dissociating the Centrosomal Matrix Protein AKAP450 from Centrioles Impairs Centriole Duplication and Cell Cycle Progression

Guy Keryer ^* , Oliwia Witczak ^*  , Annie Delouvée , Wolfram A. Kemmner  , Danielle Rouillard , Kjetil Taskén  , and Michel Bornens  ^||

Unité Mixte Recherche 144-Centre National de la Recherche Scientifique-Institut Curie, F-75248, Paris, France; Department of Medical Biochemistry, Institute of Basic Medical Sciences, University of Oslo, N-0317 Oslo, Norway; Department of Biochemistry, Max-Planck-Institute for Developmental Biology D-72076 Tübingen, Germany; and Unité Mixte Recherche 147-Centre National de la Recherche Scientifique-Institut Curie, F-75248, Paris, France

Submitted September 25, 2002; Revised December 27, 2002; Accepted February 5, 2003
Monitoring Editor: Marc Mumby

Centrosomes provide docking sites for regulatory molecules involved in the control of the cell division cycle. The centrosomal matrix contains several proteins, which anchor kinases and phosphatases. The large A-Kinase Anchoring Protein AKAP450 is acting as a scaffolding protein for other components of the cell signaling machinery. We selectively perturbed the centrosome by modifying the cellular localization of AKAP450. We report that the expression in HeLa cells of the C terminus of AKAP450, which contains the centrosome-targeting domain of AKAP450 but not its coiled-coil domains or binding sites for signaling molecules, leads to the displacement of the endogenous centrosomal AKAP450 without removing centriolar or pericentrosomal components such as centrin, -tubulin, or pericentrin. The centrosomal protein kinase A type II was delocalized. We further show that this expression impairs cytokinesis and increases ploidy in HeLa cells, whereas it arrests diploid RPE1 fibroblasts in G1, thus further establishing a role of the centrosome in the regulation of the cell division cycle. Moreover, centriole duplication is interrupted. Our data show that the association between centrioles and the centrosomal matrix protein AKAP450 is critical for the integrity of the centrosome and for its reproduction.

^|| Corresponding author. E-mail address: michel.bornens{at}curie.fr.

This article has been cited by other articles:

				R. Boutros, V. Lobjois, and B. Ducommun CDC25B Involvement in the Centrosome Duplication Cycle and in Microtubule Nucleation Cancer Res., December 15, 2007; 67(24): 11557 - 11564. [Abstract] [Full Text] [PDF]

				C. Kasbek, C.-H. Yang, A. M. Yusof, H. M. Chapman, M. Winey, and H. A. Fisk Preventing the Degradation of Mps1 at Centrosomes Is Sufficient to Cause Centrosome Reduplication in Human Cells Mol. Biol. Cell, November 1, 2007; 18(11): 4457 - 4469. [Abstract] [Full Text] [PDF]

				V. Srsen, N. Gnadt, A. Dammermann, and A. Merdes Inhibition of centrosome protein assembly leads to p53-dependent exit from the cell cycle J. Cell Biol., August 28, 2006; 174(5): 625 - 630. [Abstract] [Full Text] [PDF]

				W. Y. Tsang, A. Spektor, D. J. Luciano, V. B. Indjeian, Z. Chen, J. L. Salisbury, I. Sanchez, and B. D. Dynlacht CP110 Cooperates with Two Calcium-binding Proteins to Regulate Cytokinesis and Genome Stability Mol. Biol. Cell, August 1, 2006; 17(8): 3423 - 3434. [Abstract] [Full Text] [PDF]

				B. S. E. D. El Homasany, Y. Volkov, M. Takahashi, Y. Ono, G. Keryer, A. Delouvee, E. Looby, A. Long, and D. Kelleher The Scaffolding Protein CG-NAP/AKAP450 Is a Critical Integrating Component of the LFA-1-Induced Signaling Complex in Migratory T Cells J. Immunol., December 15, 2005; 175(12): 7811 - 7818. [Abstract] [Full Text] [PDF]

				T. Nishimura, M. Takahashi, H.-S. Kim, H. Mukai, and Y. Ono Centrosome-targeting region of CG-NAP causes centrosome amplification by recruiting cyclin E-cdk2 complex Genes Cells, January 1, 2005; 10(1): 75 - 86. [Abstract] [Full Text] [PDF]

				M. Martinez-Campos, R. Basto, J. Baker, M. Kernan, and J. W. Raff The Drosophila pericentrin-like protein is essential for cilia/flagella function, but appears to be dispensable for mitosis J. Cell Biol., June 7, 2004; 165(5): 673 - 683. [Abstract] [Full Text] [PDF]

				S. Dutertre, M. Cazales, M. Quaranta, C. Froment, V. Trabut, C. Dozier, G. Mirey, J.-P. Bouche, N. Theis-Febvre, E. Schmitt, et al. Phosphorylation of CDC25B by Aurora-A at the centrosome contributes to the G2-M transition J. Cell Sci., May 15, 2004; 117(12): 2523 - 2531. [Abstract] [Full Text] [PDF]

				M. Delattre and P. Gonczy The arithmetic of centrosome biogenesis J. Cell Sci., May 1, 2004; 117(9): 1619 - 1630. [Abstract] [Full Text] [PDF]

				C. Regnard, D. Fesquet, C. Janke, D. Boucher, E. Desbruyeres, A. Koulakoff, C. Insina, P. Travo, and B. Edde Characterisation of PGs1, a subunit of a protein complex co-purifying with tubulin polyglutamylase J. Cell Sci., October 15, 2003; 116(20): 4181 - 4190. [Abstract] [Full Text] [PDF]

				G. Keryer, B. Di Fiore, C. Celati, K. F. Lechtreck, M. Mogensen, A. Delouvee, P. Lavia, M. Bornens, and A.-M. Tassin Part of Ran Is Associated with AKAP450 at the Centrosome: Involvement in Microtubule-organizing Activity Mol. Biol. Cell, October 1, 2003; 14(10): 4260 - 4271. [Abstract] [Full Text] [PDF]