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Vol. 14, Issue 7, 2744-2755, July 2003

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Rpb4p, a Subunit of RNA Polymerase II, Mediates mRNA Export during Stress

Marganit Farago ^* , Tal Nahari ^* , Christopher Hammel , Charles N. Cole , and Mordechai Choder ^*  ¶

^*Department of Molecular Microbiology and Biotechnology, Tel-Aviv University, Ramat Aviv 69978, Israel; Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755; and Department of Molecular Microbiology, the Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa 31096, Israel

Submitted November 18, 2002; Revised February 17, 2003; Accepted February 17, 2003
Monitoring Editor: Pamela A. Silver

Changes in gene expression represent a major mechanism by which cells respond to stress. We and other investigators have previously shown that the yeast RNA polymerase II subunit Rpb4p is required for transcription under various stress conditions, but not under optimal growth conditions. Here we show that, in addition to its role in transcription, Rpb4p is also required for mRNA export, but only when cells are exposed to stress conditions. The roles of Rpb4p in transcription and in mRNA export can be uncoupled genetically by specific mutations in Rpb4p. Both functions of Rpb4p are required to maintain cell viability during stress. We propose that Rpb4p participates in the cellular responses to stress at the interface of the transcription and the export machineries.

Both authors contributed equally to this work.

^¶ Corresponding author. E-mail address: choder{at}tx.technion.ac.il.

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