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Vol. 14, Issue 7, 2793-2808, July 2003

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Fission Yeast cdc31p Is a Component of the Half-bridge and Controls SPB Duplication

Anne Paoletti ^* , Nicole Bordes ^*, Raphaël Haddad ^* , Cindi L. Schwartz , Fred Chang ^¶, and Michel Bornens ^*

^*Institut Curie, UMR144 CNRS, 75248 Paris Cedex 05 France; Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80309-0347; and ^¶Department of Microbiology, Columbia University, New York, New York 10032

Submitted October 17, 2002; Revised February 28, 2003; Accepted March 3, 2003
Monitoring Editor: Tim Stearns

The fission yeast spindle pole body (SPB) is a nucleus-associated organelle that duplicates once each cell cycle during interphase. Duplicated SPBs serve as the poles of an intranuclear mitotic spindle after their insertion into the nuclear envelope in mitosis (Ding et al., Mol. Biol. Cell 8, 1461–1479). Here, we report the identification and characterization of Schizosaccharomyces pombe cdc31p, a member of the conserved calcium-binding centrin/CDC31 family. Immunofluorescence and immunoelectron microscopy show that cdc31p is a SPB component localized at the half-bridge structure of the SPB. cdc31 is an essential gene and cdc31 cells and cdc31 conditional mutant cells arrest in mitosis with a monopolar mitotic spindle organized from a single SPB. EM analysis demonstrates that mutant cdc31 cells fail to duplicate the SPB. In addition, cdc31p exhibits genetic interactions with the SPB component sad1p and is required for sad1p localization. Finally, cdc31 mutant can undergo single or multiple rounds of septation before the exit from mitosis, suggesting that cdc31p activity or SPB duplication may be required for the proper coordination between the exit from mitosis and the initiation of septation.

Abbreviations used: HU, hydroxyurea; MTs, microtubules; MTOC, microtubule organizing center; SPB, spindle pole body.

Present address: Institut de Biochimie et Génétique cellulaires, UMR 5095 CNRS 1, rue Camille Saint-Saëns 33077 Bordeaux cedex.

Corresponding author. E-mail address: paoletti{at}curie.fr.

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