![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 14, Issue 7, 2972-2983, July 2003
* Department of Molecular Biosciences, The University of Kansas, Lawrence,
Kansas 66045;
University of Tokushima, Tokushima 770-8503 Japan;
Howard Hughes Medical Institutes and Department of Cell Biology, University of
Massachusetts Medical School, Worcester, Massachusetts 01605; and
|| Department of Embryology, Carnegie Institution of Washington, Baltimore,
Maryland 21210
Submitted January 7, 2003;
Revised March 13, 2003;
Accepted March 14, 2003
Monitoring Editor: Marvin Wickens
Introduction of double-stranded RNA (dsRNA) can elicit a gene-specific RNA interference response in a variety of organisms and cell types. In many cases, this response has a systemic character in that silencing of gene expression is observed in cells distal from the site of dsRNA delivery. The molecular mechanisms underlying the mobile nature of RNA silencing are unknown. For example, although cellular entry of dsRNA is possible, cellular exit of dsRNA from normal animal cells has not been directly observed. We provide evidence that transgenic strains of Caenorhabditis elegans transcribing dsRNA from a tissue-specific promoter do not exhibit comprehensive systemic RNA interference phenotypes. In these same animals, modifications of environmental conditions can result in more robust systemic RNA silencing. Additionally, we find that genetic mutations can influence the systemic character of RNA silencing in C. elegans and can separate mechanisms underlying systemic RNA silencing into tissue-specific components. These data suggest that trafficking of RNA silencing signals in C. elegans is regulated by specific physiological and genetic factors.
Present address: University of Tokushima, Tokushima 770-8503 Japan.
Corresponding author. E-mail address:
timmons{at}ku.edu.
This article has been cited by other articles:
|
|
 |
|
  A. M. Jose, J. J. Smith, and C. P. Hunter Export of RNA silencing from C. elegans tissues does not require the RNA channel SID-1 PNAS, February 17, 2009; 106(7): 2283 - 2288. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Briese, B. Esmaeili, S. Fraboulet, E. C. Burt, S. Christodoulou, P. R. Towers, K. E. Davies, and D. B. Sattelle Deletion of smn-1, the Caenorhabditis elegans ortholog of the spinal muscular atrophy gene, results in locomotor dysfunction and reduced lifespan Hum. Mol. Genet., January 1, 2009; 18(1): 97 - 104. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P.N. Pushparaj, J.J. Aarthi, J. Manikandan, and S.D. Kumar siRNA, miRNA, and shRNA: in vivo Applications Journal of Dental Research, November 1, 2008; 87(11): 992 - 1003. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Zhou, E. Ryabov, X. Zhang, and Y. Hong Influence of viral genes on the cell-to-cell spread of RNA silencing J. Exp. Bot., July 1, 2008; 59(10): 2803 - 2813. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Han, P. Sundaram, H. Kenjale, J. Grantham, and L. Timmons The Caenorhabditis elegans rsd-2 and rsd-6 Genes Are Required for Chromosome Functions During Exposure to Unfavorable Environments Genetics, April 1, 2008; 178(4): 1875 - 1893. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Uccelletti, A. Pascoli, F. Farina, A. Alberti, P. Mancini, C. B. Hirschberg, and C. Palleschi APY-1, a Novel Caenorhabditis elegans Apyrase Involved in Unfolded Protein Response Signalling and Stress Responses Mol. Biol. Cell, April 1, 2008; 19(4): 1337 - 1345. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Vogler, R. Akbergenov, P. V. Shivaprasad, V. Dang, M. Fasler, M.-O. Kwon, S. Zhanybekova, T. Hohn, and M. Heinlein Modification of Small RNAs Associated with Suppression of RNA Silencing by Tobamovirus Replicase Protein J. Virol., October 1, 2007; 81(19): 10379 - 10388. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Tegner, J. Skogsberg, and J. Bjorkegren Thematic review series: Systems Biology Approaches to Metabolic and Cardiovascular Disorders. Multi-organ whole-genome measurements and reverse engineering to uncover gene networks underlying complex traits J. Lipid Res., February 1, 2007; 48(2): 267 - 277. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Sundaram, B. Echalier, W. Han, D. Hull, and L. Timmons ATP-binding Cassette Transporters Are Required for Efficient RNA Interference in Caenorhabditis elegans Mol. Biol. Cell, August 1, 2006; 17(8): 3678 - 3688. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.P. HUNTER, W.M. WINSTON, C. MOLODOWITCH, E.H. FEINBERG, J. SHIH, M. SUTHERLIN, A.J. WRIGHT, and M.C. FITZGERALD Systemic RNAi in Caenorhabditis elegans Cold Spring Harb Symp Quant Biol, January 1, 2006; 71(0): 95 - 100. [Abstract] [PDF]
|
|
|
|
 |
|
 H. Murakami, K. Bessinger, J. Hellmann, and S. Murakami Aging-Dependent and -Independent Modulation of Associative Learning Behavior by Insulin/Insulin-Like Growth Factor-1 Signal in Caenorhabditis elegans J. Neurosci., November 23, 2005; 25(47): 10894 - 10904. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Cao, P. Zhou, X. Zhang, S. Zhu, X. Zhong, Q. Xiao, B. Ding, and Y. Li Identification of an RNA Silencing Suppressor from a Plant Double-Stranded RNA Virus J. Virol., October 15, 2005; 79(20): 13018 - 13027. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. A. Zerhouni US Biomedical Research: Basic, Translational, and Clinical Sciences JAMA, September 21, 2005; 294(11): 1352 - 1358. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. D. Buckingham, B. Esmaeili, M. Wood, and D. B. Sattelle RNA interference: from model organisms towards therapy for neural and neuromuscular disorders Hum. Mol. Genet., October 1, 2004; 13(suppl_2): R275 - R288. [Abstract] [Full Text] [PDF]
|
|
