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Vol. 14, Issue 8, 3180-3191, August 2003

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A Novel Variant of Cardiac Myosin-binding Protein-C That Is Unable to Assemble into Sarcomeres Is Expressed in the Aged Mouse Atrium

Naruki Sato ^* , Tsutomu Kawakami , Ayako Nakayama , Hiroyuki Suzuki , Hideko Kasahara , and Takashi Obinata ^*  

^* Department of Biology, Faculty of Science, Chiba University, Yayoi-cho, Inage-ku, Chiba 263-8522, Japan; Graduate School of Science and Technology, Chiba University, Yayoi-cho, Inage-ku, Chiba 263-8522, Japan; and Department of Physiology and Functional Genomics, College of Medicine, University of Florida, Gainesville, Florida 32610-0274

Submitted October 25, 2002; Revised March 3, 2003; Accepted April 9, 2003
Monitoring Editor: Mary Beckerle

Cardiac myosin-binding protein-C (MyBP-C), also known as C-protein, is one of the major myosin-binding proteins localizing at A-bands. MyBP-C has three isoforms encoded by three distinct genes: fast-skeletal, slow-skeletal, and cardiac type. Herein, we are reporting a novel alternative spliced form of cardiac MyBP-C, MyBP-C(+), which includes an extra 30 nucleotides, encoding 10 amino acids in the carboxyl-terminal connectin/titin binding region. This alternative spliced form of MyBP-C(+) has a markedly decreased binding affinity to myosin filaments and connectin/titin in vitro and does not localize to A-bands in cardiac myocytes. When MyBP-C(+) was expressed in chicken cardiac myocytes, sarcomere structure was markedly disorganized, suggesting it has possible dominant negative effects on sarcomere organization. Expression of MyBP-C(+) is hardly detected in ventricles through cardiac development, but its expression gradually increases in atria and becomes the dominant form after 6 mo of age. The present study demonstrates an age-induced new isoform of cardiac MyBP-C harboring possible dominant negative effects on sarcomere assembly.

Corresponding author. E-mail address: sato{at}bio.s.chiba-u.ac.jp or tobinata{at}bio.s.chiba-u.ac.jp.

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