QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

Vol. 14, Issue 8, 3192-3207, August 2003

This Article Full Text Full Text (PDF) All Versions of this Article: E03-03-0166v1 14/8/3192    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Brown, J. M. Articles by Gaertig, J. Search for Related Content PubMed PubMed Citation Articles by Brown, J. M. Articles by Gaertig, J.

Hypoxia Regulates Assembly of Cilia in Suppressors of Tetrahymena Lacking an Intraflagellar Transport Subunit Gene

Jason M. Brown ^* , Noah A. Fine , Gautham Pandiyan ^*, Rupal Thazhath ^*, and Jacek Gaertig ^* 

^* Department of Cellular Biology, University of Georgia, Athens, Georgia 30602; Department of Biology, York University, Toronto, Ontario, Canada M3J 1P3

Submitted March 21, 2003; Accepted April 2, 2003
Monitoring Editor: Paul Matsudaira

We cloned a Tetrahymena thermophila gene, IFT52, encoding a homolog of the Chlamydomonas intraflagellar transport protein, IFT52. Disruption of IFT52 led to loss of cilia and incomplete cytokinesis, a phenotype indistinguishable from that of mutants lacking kinesin-II, a known ciliary assembly transporter. The cytokinesis failures seem to result from lack of cell movement rather than from direct involvement of ciliary assembly pathway components in cytokinesis. Spontaneous partial suppressors of the IFT52 null mutants occurred, which assembled cilia at high cell density and resorbed cilia at low cell density. The stimulating effect of high cell density on cilia formation is based on the creation of pericellular hypoxia. Thus, at least under certain conditions, ciliary assembly is affected by an extracellular signal and the Ift52p function may be integrated into signaling pathways that regulate ciliogenesis.

Abbreviations used: IFT, intraflagellar transport; MAC, macronucleus; MIC, micronucleus; WT, wild-type.

Present address: Department of Biology, Young Harris College, Young Harris, GA 30582.

Corresponding author. E-mail address: jgaertig{at}cb.uga.edu.

This article has been cited by other articles:

				J. C. Hoeng, S. C. Dawson, S. A. House, M. S. Sagolla, J. K. Pham, J. J. Mancuso, J. Lowe, and W. Z. Cande High-Resolution Crystal Structure and In Vivo Function of a Kinesin-2 Homologue in Giardia intestinalis Mol. Biol. Cell, July 1, 2008; 19(7): 3124 - 3137. [Abstract] [Full Text] [PDF]

				C.-C. Tsao and M. A. Gorovsky Different Effects of Tetrahymena IFT172 Domains on Anterograde and Retrograde Intraflagellar Transport Mol. Biol. Cell, April 1, 2008; 19(4): 1450 - 1461. [Abstract] [Full Text] [PDF]

				S. Absalon, T. Blisnick, L. Kohl, G. Toutirais, G. Dore, D. Julkowska, A. Tavenet, and P. Bastin Intraflagellar Transport and Functional Analysis of Genes Required for Flagellum Formation in Trypanosomes Mol. Biol. Cell, March 1, 2008; 19(3): 929 - 944. [Abstract] [Full Text] [PDF]

				C.-C. Tsao and M. A. Gorovsky Tetrahymena IFT122A is not essential for cilia assembly but plays a role in returning IFT proteins from the ciliary tip to the cell body J. Cell Sci., February 15, 2008; 121(4): 428 - 436. [Abstract] [Full Text] [PDF]

				S. Griffiths, N. Portman, P. R. Taylor, S. Gordon, M. L. Ginger, and K. Gull RNA Interference Mutant Induction In Vivo Demonstrates the Essential Nature of Trypanosome Flagellar Function during Mammalian Infection Eukaryot. Cell, July 1, 2007; 6(7): 1248 - 1250. [Abstract] [Full Text] [PDF]

				Y. Hou, H. Qin, J. A. Follit, G. J. Pazour, J. L. Rosenbaum, and G. B. Witman Functional analysis of an individual IFT protein: IFT46 is required for transport of outer dynein arms into flagella J. Cell Biol., February 26, 2007; 176(5): 653 - 665. [Abstract] [Full Text] [PDF]

				D. Wloga, A. Camba, K. Rogowski, G. Manning, M. Jerka-Dziadosz, and J. Gaertig Members of the NIMA-related Kinase Family Promote Disassembly of Cilia by Multiple Mechanisms Mol. Biol. Cell, June 1, 2006; 17(6): 2799 - 2810. [Abstract] [Full Text] [PDF]

				N. E. Williams, C.-C. Tsao, J. Bowen, G. L. Hehman, R. J. Williams, and J. Frankel The Actin Gene ACT1 Is Required for Phagocytosis, Motility, and Cell Separation of Tetrahymena thermophila Eukaryot. Cell, March 1, 2006; 5(3): 555 - 567. [Abstract] [Full Text] [PDF]

				Y. Shang, C.-C. Tsao, and M. A. Gorovsky Mutational analyses reveal a novel function of the nucleotide-binding domain of {gamma}-tubulin in the regulation of basal body biogenesis J. Cell Biol., December 19, 2005; 171(6): 1035 - 1044. [Abstract] [Full Text] [PDF]

				R. Mussmann, M. Engstler, H. Gerrits, R. Kieft, C. B. Toaldo, J. Onderwater, H. Koerten, H. G. A. M. van Luenen, and P. Borst Factors Affecting the Level and Localization of the Transferrin Receptor in Trypanosoma brucei J. Biol. Chem., September 24, 2004; 279(39): 40690 - 40698. [Abstract] [Full Text] [PDF]

				R. Thazhath, M. Jerka-Dziadosz, J. Duan, D. Wloga, M. A. Gorovsky, J. Frankel, and J. Gaertig Cell Context-specific Effects of the {beta}-Tubulin Glycylation Domain on Assembly and Size of Microtubular Organelles Mol. Biol. Cell, September 1, 2004; 15(9): 4136 - 4147. [Abstract] [Full Text] [PDF]