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Vol. 14, Issue 8, 3192-3207, August 2003

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Hypoxia Regulates Assembly of Cilia in Suppressors of Tetrahymena Lacking an Intraflagellar Transport Subunit Gene

Jason M. Brown ^* , Noah A. Fine , Gautham Pandiyan ^*, Rupal Thazhath ^*, and Jacek Gaertig ^* 

^* Department of Cellular Biology, University of Georgia, Athens, Georgia 30602; Department of Biology, York University, Toronto, Ontario, Canada M3J 1P3

Submitted March 21, 2003; Accepted April 2, 2003
Monitoring Editor: Paul Matsudaira

We cloned a Tetrahymena thermophila gene, IFT52, encoding a homolog of the Chlamydomonas intraflagellar transport protein, IFT52. Disruption of IFT52 led to loss of cilia and incomplete cytokinesis, a phenotype indistinguishable from that of mutants lacking kinesin-II, a known ciliary assembly transporter. The cytokinesis failures seem to result from lack of cell movement rather than from direct involvement of ciliary assembly pathway components in cytokinesis. Spontaneous partial suppressors of the IFT52 null mutants occurred, which assembled cilia at high cell density and resorbed cilia at low cell density. The stimulating effect of high cell density on cilia formation is based on the creation of pericellular hypoxia. Thus, at least under certain conditions, ciliary assembly is affected by an extracellular signal and the Ift52p function may be integrated into signaling pathways that regulate ciliogenesis.

Abbreviations used: IFT, intraflagellar transport; MAC, macronucleus; MIC, micronucleus; WT, wild-type.

Present address: Department of Biology, Young Harris College, Young Harris, GA 30582.

Corresponding author. E-mail address: jgaertig{at}cb.uga.edu.

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