QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

Vol. 14, Issue 8, 3494-3505, August 2003

This Article Full Text Full Text (PDF) All Versions of this Article: E02-10-0655v1 14/8/3494    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Yoder, T. J. Articles by Davis, T. N. Search for Related Content PubMed PubMed Citation Articles by Yoder, T. J. Articles by Davis, T. N.

The Saccharomyces cerevisiae Spindle Pole Body Is a Dynamic Structure

Tennessee J. Yoder ^*, Chad G. Pearson , Kerry Bloom , and Trisha N. Davis ^* 

^* Program in Molecular and Cellular Biology and Department of Biochemistry, University of Washington, Seattle, Washington 98195; Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599

Submitted October 15, 2002; Revised April 2, 2003; Accepted April 2, 2003
Monitoring Editor: Tim Stearns

During spindle pole body (SPB) duplication, the new SPB is assembled at a distinct site adjacent to the old SPB. Using quantitative fluorescence methods, we studied the assembly and dynamics of the core structural SPB component Spc110p. The SPB core exhibits both exchange and growth in a cell cycle-dependent manner. During G1/S phase, the old SPB exchanges 50% of old Spc110p for new Spc110p. In G2 little Spc110p is exchangeable. Thus, Spc110p is dynamic during G1/S and becomes stable during G2. The SPB incorporates additional Spc110p in late G2 and M phases; this growth is followed by reduction in the next G1. Spc110p addition to the SPBs (growth) also occurs in response to G2 and mitotic arrests but not during a G1 arrest. Our results reveal several dynamic features of the SPB core: cell cycle-dependent growth and reduction, growth in response to cell cycle arrests, and exchange of Spc110p during SPB duplication. Moreover, rather than being considered a conservative or dispersive process, the assembly of Spc110p into the SPB is more readily considered in terms of growth and exchange.

Article published online ahead of print. Mol. Biol. Cell 10.1091/mbc.E02-10-0655. Article and publication date are available at www.molbiolcell.org/cgi/doi/10.1091/mbc.E02-10-0655.

Corresponding author. E-mail address: tdavis{at}u.washington.edu.

This article has been cited by other articles:

				J. K. Moore and R. K. Miller The Cyclin-dependent Kinase Cdc28p Regulates Multiple Aspects of Kar9p Function in Yeast Mol. Biol. Cell, April 1, 2007; 18(4): 1187 - 1202. [Abstract] [Full Text] [PDF]

				J. K. Moore, S. D'Silva, and R. K. Miller The CLIP-170 Homologue Bik1p Promotes the Phosphorylation and Asymmetric Localization of Kar9p Mol. Biol. Cell, January 1, 2006; 17(1): 178 - 191. [Abstract] [Full Text] [PDF]

				M. Niepel, C. Strambio-de-Castillia, J. Fasolo, B. T. Chait, and M. P. Rout The nuclear pore complex-associated protein, Mlp2p, binds to the yeast spindle pole body and promotes its efficient assembly J. Cell Biol., July 18, 2005; 170(2): 225 - 235. [Abstract] [Full Text] [PDF]

				E. G.D. Muller, B. E. Snydsman, I. Novik, D. W. Hailey, D. R. Gestaut, C. A. Niemann, E. T. O'Toole, T. H. Giddings Jr, B. A. Sundin, and T. N. Davis The Organization of the Core Proteins of the Yeast Spindle Pole Body Mol. Biol. Cell, July 1, 2005; 16(7): 3341 - 3352. [Abstract] [Full Text] [PDF]

				T. J. Yoder, M. A. McElwain, S. E. Francis, J. Bagley, E. G.D. Muller, B. Pak, E. T. O'Toole, M. Winey, and T. N. Davis Analysis of a Spindle Pole Body Mutant Reveals a Defect in Biorientation and Illuminates Spindle Forces Mol. Biol. Cell, January 1, 2005; 16(1): 141 - 152. [Abstract] [Full Text] [PDF]