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Vol. 15, Issue 1, 397-406, January 2004
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* Humangenetik fuer Biologen, Johann Wolfgang Goethe-Universität, D-60054 Frankfurt, Germany;
Bürgerhospital Frankfurt, Section of Gynecological Endocrinology and Fertility Surgery, D-60318 Frankfurt, Germany
Submitted May 7, 2003;
Revised September 1, 2003;
Accepted September 9, 2003
Monitoring Editor: Mary Beckerle
While searching for potential candidate molecules relevant for the pathogenesis of endometriosis, we discovered a 2910-base pair cDNA encoding a novel putative 411-amino acid integral membrane protein that we called shrew-1. The putative open-reading frame was confirmed with antibodies against shrew-1 peptides that labeled a protein of 
48 kDa in extracts of shrew-1 mRNA-positive tissue and also detected ectopically expressed shrew-1. Expression of epitope-tagged shrew-1 in epithelial cells and analysis by surface biotinylation and immunoblots demonstrated that shrew-1 is indeed a transmembrane protein. Shrew-1 is able to target to E-cadherin-mediated adherens junctions and interact with the E-cadherin–catenin complex in polarized MCF7 and Madin-Darby canine kidney cells, but not with the N-cadherin–catenin complex in nonpolarized epithelial cells. Direct interaction of shrew-1 with 
-catenin in in vitro pull-down assay suggests that -catenin might be one of the proteins that targets and/or retains shrew-1 in the adherens junctions. Interestingly, shrew-1 was partially translocated in response to scatter factor (ligand of receptor tyrosine kinase c-met) from the plasma membrane to the cytoplasm where it still colocalized with endogenous E-cadherin. In summary, we introduce shrew-1 as a novel component of adherens junctions, interacting with E-cadherin–-catenin complexes in polarized epithelial cells.
Corresponding author. E-mail address: starzinski-powitz{at}em.uni-frankfurt.de.
This article has been cited by other articles:
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 A. Coxon, K. Rex, S. Meyer, J. Sun, J. Sun, Q. Chen, R. Radinsky, R. Kendall, and T. L. Burgess Soluble c-Met receptors inhibit phosphorylation of c-Met and growth of hepatocyte growth factor: c-Met-dependent tumors in animal models Mol. Cancer Ther., May 1, 2009; 8(5): 1119 - 1125. [Abstract] [Full Text] [PDF]
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 E. M. Grund, D. Kagan, C. A. Tran, A. Zeitvogel, A. Starzinski-Powitz, S. Nataraja, and S. S. Palmer Tumor Necrosis Factor-{alpha} Regulates Inflammatory and Mesenchymal Responses via Mitogen-Activated Protein Kinase Kinase, p38, and Nuclear Factor {kappa}B in Human Endometriotic Epithelial Cells Mol. Pharmacol., May 1, 2008; 73(5): 1394 - 1404. [Abstract] [Full Text] [PDF]
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A. Schreiner, M. Ruonala, V. Jakob, J. Suthaus, E. Boles, F. Wouters, and A. Starzinski-Powitz Junction Protein Shrew-1 Influences Cell Invasion and Interacts with Invasion-promoting Protein CD147 Mol. Biol. Cell, April 1, 2007; 18(4): 1272 - 1281. [Abstract] [Full Text] [PDF]
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V. Jakob, A. Schreiner, R. Tikkanen, and A. Starzinski-Powitz Targeting of Transmembrane Protein Shrew-1 to Adherens Junctions Is Controlled by Cytoplasmic Sorting Motifs Mol. Biol. Cell, August 1, 2006; 17(8): 3397 - 3408. [Abstract] [Full Text] [PDF]
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