![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 15, Issue 10, 4382-4394, October 2004
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
* Department of Cell Biology, University of Massachusetts Medical School, Worcester, MA 01655;
Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605
Submitted May 8, 2004;
Revised July 9, 2004;
Accepted July 12, 2004
Monitoring Editor: Paul Matsudaira
Intraflagellar transport (IFT), the bidirectional movement of particles along flagella, is essential for flagellar assembly. The motor for retrograde IFT in Chlamydomonas is cytoplasmic dynein 1b, which contains the dynein heavy chain DHC1b and the light intermediate chain (LIC) D1bLIC. To investigate a possible role for the LIC in IFT, we identified a d1blic mutant. DHC1b is reduced in the mutant, indicating that D1bLIC is important for stabilizing dynein 1b. The mutant has variable length flagella that accumulate IFT-particle proteins, indicative of a defect in retrograde IFT. Interestingly, the remaining DHC1b is normally distributed in the mutant flagella, strongly suggesting that the defect is in binding of cargo to the retrograde motor rather than in motor activity per se. Cell growth and Golgi apparatus localization and morphology are normal in the mutant, indicating that D1bLIC is involved mainly in retrograde IFT. Like mammalian LICs, D1bLIC has a phosphate-binding domain (P-loop) at its N-terminus. To investigate the function of this conserved domain, d1blic mutant cells were transformed with constructs designed to express D1bLIC proteins with mutated P-loops. The constructs rescued the mutant cells to a wild-type phenotype, indicating that the function of D1bLIC in IFT is independent of its P-loop.
Abbreviations used: BAC, bacterial artificial chromosome; DHC, dynein heavy chain; DIC, differential interference contrast; EM, electron microscopy; EST, expressed sequence tag; HA, influenza hemagglutinin epitope; IFT, intraflagellar transport; LIC, light intermediate chain; P-loop, phosphate-binding domain.
Corresponding author. E-mail address: george.witman{at}umassmed.edu.
This article has been cited by other articles:
|
|
 |
|
  P. Yang, C. Yang, M. Wirschell, and S. Davis Novel LC8 Mutations Have Disparate Effects on the Assembly and Stability of Flagellar Complexes J. Biol. Chem., November 6, 2009; 284(45): 31412 - 31421. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. L. Krock, I. Mills-Henry, and B. D. Perkins Retrograde Intraflagellar Transport by Cytoplasmic Dynein-2 Is Required for Outer Segment Extension in Vertebrate Photoreceptors but Not Arrestin Translocation Invest. Ophthalmol. Vis. Sci., November 1, 2009; 50(11): 5463 - 5471. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Iomini, L. Li, J. M. Esparza, and S. K. Dutcher Retrograde Intraflagellar Transport Mutants Identify Complex A Proteins With Multiple Genetic Interactions in Chlamydomonas reinhardtii Genetics, November 1, 2009; 183(3): 885 - 896. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Huang, D. R. Diener, and J. L. Rosenbaum The ubiquitin conjugation system is involved in the disassembly of cilia and flagella J. Cell Biol., August 24, 2009; 186(4): 601 - 613. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Rajagopalan, A. Subramanian, D. E. Wilkes, D. G. Pennock, and D. J. Asai Dynein-2 Affects the Regulation of Ciliary Length but Is Not Required for Ciliogenesis in Tetrahymena thermophila Mol. Biol. Cell, January 1, 2009; 20(2): 708 - 720. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. S. Shah, S. L. Farmen, T. O. Moninger, T. R. Businga, M. P. Andrews, K. Bugge, C. C. Searby, D. Nishimura, K. A. Brogden, J. N. Kline, et al. Loss of Bardet-Biedl syndrome proteins alters the morphology and function of motile cilia in airway epithelia PNAS, March 4, 2008; 105(9): 3380 - 3385. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-C. Tsao and M. A. Gorovsky Tetrahymena IFT122A is not essential for cilia assembly but plays a role in returning IFT proteins from the ciliary tip to the cell body J. Cell Sci., February 15, 2008; 121(4): 428 - 436. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Rompolas, L. B. Pedersen, R. S. Patel-King, and S. M. King Chlamydomonas FAP133 is a dynein intermediate chain associated with the retrograde intraflagellar transport motor J. Cell Sci., October 15, 2007; 120(20): 3653 - 3665. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L.-W. Tam, N. F. Wilson, and P. A. Lefebvre A CDK-related kinase regulates the length and assembly of flagella in Chlamydomonas J. Cell Biol., March 12, 2007; 176(6): 819 - 829. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Hou, H. Qin, J. A. Follit, G. J. Pazour, J. L. Rosenbaum, and G. B. Witman Functional analysis of an individual IFT protein: IFT46 is required for transport of outer dynein arms into flagella J. Cell Biol., February 26, 2007; 176(5): 653 - 665. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.-F. Lechtreck and G. B. Witman Chlamydomonas reinhardtii hydin is a central pair protein required for flagellar motility J. Cell Biol., February 12, 2007; 176(4): 473 - 482. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. F. Lucker, R. H. Behal, H. Qin, L. C. Siron, W. D. Taggart, J. L. Rosenbaum, and D. G. Cole Characterization of the Intraflagellar Transport Complex B Core: DIRECT INTERACTION OF THE IFT81 AND IFT74/72 SUBUNITS J. Biol. Chem., July 29, 2005; 280(30): 27688 - 27696. [Abstract] [Full Text] [PDF]
|
|
