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Vol. 15, Issue 10, 4490-4499, October 2004

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Transcription Profiling of Cyclic AMP Signaling in Candida albicans

Doreen Harcus ^*, André Nantel ^* , Anne Marcil ^*, Tracey Rigby ^*, and Malcolm Whiteway ^*  

^* Genetics Group, Biotechnology Research Institute, National Research Council of Canada, Montreal, Quebec, Canada H4P 2R2; Department of Anatomy and Cell Biology, McGill University, Montreal, Quebec, Canada H3A 2B2; and Department of Biology, McGill University, Montreal, Quebec, Canada H3A 1B1

Submitted February 23, 2004; Revised July 8, 2004; Accepted July 12, 2004
Monitoring Editor: Keith Yamamoto

We used transcription profiling in Candida albicans to investigate cellular regulation involving cAMP. We found that many genes require the adenylyl cyclase Cdc35p for proper expression. These include genes encoding ribosomal subunit proteins and RNA polymerase subunit proteins, suggesting that growth could be controlled in part by cAMP-mediated modulation of gene expression. Other genes influenced by loss of adenylyl cyclase are involved in metabolism, the cell wall, and stress response and include a group of genes of unknown function that are unique to C. albicans. The profiles generated by loss of the adenylyl cyclase regulator Ras1p and a downstream effector Efg1p were also examined. The loss of Ras1p function disturbs the expression of a subset of the genes regulated by adenylyl cyclase, suggesting both that the primary role of Ras1p in transcriptional regulation involves its influence on the function of Cdc35p and that there are Ras1p independent roles for Cdc35p. The transcription factor Efg1p is also needed for the expression of many genes; however, these genes are distinct from those modulated by Cdc35p with the exception of a class of hyphal-specific genes. Therefore transcription profiling establishes that cAMP plays a key role in the overall regulation of gene expression in C. albicans, and enhances our detailed understanding of the circuitry controlling this regulation.

The online version of this article contains supplemental material accessible through http://www.molbiolcell.org.

Corresponding author. E-mail address: malcolm.whiteway{at}cnrc-nrc.gc.ca.

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