QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

Vol. 15, Issue 10, 4568-4583, October 2004

This Article Full Text Full Text (PDF) All Versions of this Article: E04-04-0330v1 15/10/4568    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Versele, M. Articles by Thorner, J. Search for Related Content PubMed PubMed Citation Articles by Versele, M. Articles by Thorner, J.

Protein–Protein Interactions Governing Septin Heteropentamer Assembly and Septin Filament Organization in Saccharomyces cerevisiae

Matthias Versele ^*, Björn Gullbrand, Mark J. Shulewitz , Victor J. Cid , Shirin Bahmanyar , Raymond E. Chen, Patrick Barth, Tom Alber, and Jeremy Thorner ^||

Division of Biochemistry and Molecular Biology, Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720-3202

Submitted April 21, 2004; Revised July 14, 2004; Accepted July 16, 2004
Monitoring Editor: Anthony Bretscher

Mitotic yeast (Saccharomyces cerevisiae) cells express five related septins (Cdc3, Cdc10, Cdc11, Cdc12, and Shs1) that form a cortical filamentous collar at the mother-bud neck necessary for normal morphogenesis and cytokinesis. All five possess an N-terminal GTPase domain and, except for Cdc10, a C-terminal extension (CTE) containing a predicted coiled coil. Here, we show that the CTEs of Cdc3 and Cdc12 are essential for their association and for the function of both septins in vivo. Cdc10 interacts with a Cdc3–Cdc12 complex independently of the CTE of either protein. In contrast to Cdc3 and Cdc12, the Cdc11 CTE, which recruits the nonessential septin Shs1, is dispensable for its function in vivo. In addition, Cdc11 forms a stoichiometric complex with Cdc12, independent of its CTE. Reconstitution of various multiseptin complexes and electron microscopic analysis reveal that Cdc3, Cdc11, and Cdc12 are all necessary and sufficient for septin filament formation, and presence of Cdc10 causes filament pairing. These data provide novel insights about the connectivity among the five individual septins in functional septin heteropentamers and the organization of septin filaments.

Abbreviations used: CTE, C-terminal extension; NTA, nitrilotriacetate.

^* Present address: Laboratory of Molecular Cell Biology, Institute of Botany and Microbiology, Katholieke Universiteit Leuven, Leuven-Heverlee B-3001, Belgium

Present address: Genentech, Inc., South San Francisco, CA 94080

Present address: Department of Microbiology, School of Pharmacy, University of Madrid Complutense, Madrid 28040, Spain

Present address: Department of Biological Sciences, Stanford University, Stanford, CA 94305.

^|| Corresponding author. E-mail address: jeremy{at}socrates.berkeley.edu.

This article has been cited by other articles:

				S. Nagaraj, A. Rajendran, C. E. Jackson, and M. S. Longtine Role of Nucleotide Binding in Septin-Septin Interactions and Septin Localization in Saccharomyces cerevisiae Mol. Cell. Biol., August 15, 2008; 28(16): 5120 - 5137. [Abstract] [Full Text] [PDF]

				J. P. Bharucha, J. R. Larson, J. B. Konopka, and K. Tatchell Saccharomyces cerevisiae Afr1 Protein Is a Protein Phosphatase 1/Glc7-Targeting Subunit That Regulates the Septin Cytoskeleton during Mating Eukaryot. Cell, August 1, 2008; 7(8): 1246 - 1255. [Abstract] [Full Text] [PDF]

				A. Bertin, M. A. McMurray, P. Grob, S.-S. Park, G. Garcia III, I. Patanwala, H.-l. Ng, T. Alber, J. Thorner, and E. Nogales Saccharomyces cerevisiae septins: Supramolecular organization of heterooligomers and the mechanism of filament assembly PNAS, June 17, 2008; 105(24): 8274 - 8279. [Abstract] [Full Text] [PDF]

				A. Gonzalez-Novo, J. Correa-Bordes, L. Labrador, M. Sanchez, C. R. Vazquez de Aldana, and J. Jimenez Sep7 Is Essential to Modify Septin Ring Dynamics and Inhibit Cell Separation during Candida albicans Hyphal Growth Mol. Biol. Cell, April 1, 2008; 19(4): 1509 - 1518. [Abstract] [Full Text] [PDF]

				H.-O. Park and E. Bi Central Roles of Small GTPases in the Development of Cell Polarity in Yeast and Beyond Microbiol. Mol. Biol. Rev., March 1, 2007; 71(1): 48 - 96. [Abstract] [Full Text] [PDF]

				C. Low and I. G. Macara Structural Analysis of Septin 2, 6, and 7 Complexes J. Biol. Chem., October 13, 2006; 281(41): 30697 - 30706. [Abstract] [Full Text] [PDF]

				S. Asano, J.-E. Park, L.-R. Yu, M. Zhou, K. Sakchaisri, C. J. Park, Y. H. Kang, J. Thorner, T. D. Veenstra, and K. S. Lee Direct Phosphorylation and Activation of a Nim1-related Kinase Gin4 by Elm1 in Budding Yeast J. Biol. Chem., September 15, 2006; 281(37): 27090 - 27098. [Abstract] [Full Text] [PDF]

				M. Iwase, J. Luo, S. Nagaraj, M. Longtine, H. B. Kim, B. K. Haarer, C. Caruso, Z. Tong, J. R. Pringle, and E. Bi Role of a Cdc42p Effector Pathway in Recruitment of the Yeast Septins to the Presumptive Bud Site Mol. Biol. Cell, March 1, 2006; 17(3): 1110 - 1125. [Abstract] [Full Text] [PDF]

				L. Kozubowski, J. R. Larson, and K. Tatchell Role of the Septin Ring in the Asymmetric Localization of Proteins at the Mother-Bud Neck in Saccharomyces cerevisiae Mol. Biol. Cell, August 1, 2005; 16(8): 3455 - 3466. [Abstract] [Full Text] [PDF]

				A. A. Rodal, L. Kozubowski, B. L. Goode, D. G. Drubin, and J. H. Hartwig Actin and Septin Ultrastructures at the Budding Yeast Cell Cortex Mol. Biol. Cell, January 1, 2005; 16(1): 372 - 384. [Abstract] [Full Text] [PDF]

				H. An, J. L. Morrell, J. L. Jennings, A. J. Link, and K. L. Gould Requirements of Fission Yeast Septins for Complex Formation, Localization, and Function Mol. Biol. Cell, December 1, 2004; 15(12): 5551 - 5564. [Abstract] [Full Text] [PDF]