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Vol. 15, Issue 10, 4568-4583, October 2004
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Division of Biochemistry and Molecular Biology, Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720-3202
Submitted April 21, 2004;
Revised July 14, 2004;
Accepted July 16, 2004
Monitoring Editor: Anthony Bretscher
Mitotic yeast (Saccharomyces cerevisiae) cells express five related septins (Cdc3, Cdc10, Cdc11, Cdc12, and Shs1) that form a cortical filamentous collar at the mother-bud neck necessary for normal morphogenesis and cytokinesis. All five possess an N-terminal GTPase domain and, except for Cdc10, a C-terminal extension (CTE) containing a predicted coiled coil. Here, we show that the CTEs of Cdc3 and Cdc12 are essential for their association and for the function of both septins in vivo. Cdc10 interacts with a Cdc3–Cdc12 complex independently of the CTE of either protein. In contrast to Cdc3 and Cdc12, the Cdc11 CTE, which recruits the nonessential septin Shs1, is dispensable for its function in vivo. In addition, Cdc11 forms a stoichiometric complex with Cdc12, independent of its CTE. Reconstitution of various multiseptin complexes and electron microscopic analysis reveal that Cdc3, Cdc11, and Cdc12 are all necessary and sufficient for septin filament formation, and presence of Cdc10 causes filament pairing. These data provide novel insights about the connectivity among the five individual septins in functional septin heteropentamers and the organization of septin filaments.
Abbreviations used: CTE, C-terminal extension; NTA, nitrilotriacetate.
* Present address: Laboratory of Molecular Cell Biology, Institute of Botany and Microbiology, Katholieke Universiteit Leuven, Leuven-Heverlee B-3001, Belgium
Present address: Genentech, Inc., South San Francisco, CA 94080
Present address: Department of Microbiology, School of Pharmacy, University of Madrid Complutense, Madrid 28040, Spain
Present address: Department of Biological Sciences, Stanford University, Stanford, CA 94305.
|| Corresponding author. E-mail address: jeremy{at}socrates.berkeley.edu.
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