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Vol. 15, Issue 10, 4633-4646, October 2004

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The LC7 Light Chains of Chlamydomonas Flagellar Dyneins Interact with Components Required for Both Motor Assembly and Regulation

Linda M. DiBella ^*, Miho Sakato ^*, Ramila S. Patel-King ^*, Gregory J. Pazour , and Stephen M. King ^* 

^* Departments of Biochemistry, and Molecular, Microbial, and Structural Biology, University of Connecticut Health Center, Farmington, CT 06030-3305; Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605

Submitted June 8, 2004; Revised July 9, 2004; Accepted July 23, 2004
Monitoring Editor: Tim Stearns

Members of the LC7/Roadblock family of light chains (LCs) have been found in both cytoplasmic and axonemal dyneins. LC7a was originally identified within Chlamydomonas outer arm dynein and associates with this motor's cargo-binding region. We describe here a novel member of this protein family, termed LC7b that is also present in the Chlamydomonas flagellum. Levels of LC7b are reduced 20% in axonemes isolated from strains lacking inner arm I1 and are 80% lower in the absence of the outer arms. When both dyneins are missing, LC7b levels are diminished to <10%. In oda9 axonemal extracts that completely lack outer arms, LC7b copurifies with inner arm I1, whereas in ida1 extracts that are devoid of I1 inner arms it associates with outer arm dynein. We also have observed that some LC7a is present in both isolated axonemes and purified 18S dynein from oda1, suggesting that it is also a component of both the outer arm and inner arm I1. Intriguingly, in axonemal extracts from the LC7a null mutant, oda15, which assembles 30% of its outer arms, LC7b fails to copurify with either dynein, suggesting that it interacts with LC7a. Furthermore, both the outer arm heavy chain and DC2 from the outer arm docking complex completely dissociate after salt extraction from oda15 axonemes. EDC cross-linking of purified dynein revealed that LC7b interacts with LC3, an outer dynein arm thioredoxin; DC2, an outer arm docking complex component; and also with the phosphoprotein IC138 from inner arm I1. These data suggest that LC7a stabilizes both the outer arms and inner arm I1 and that both LC7a and LC7b are involved in multiple intradynein interactions within both dyneins.

Abbreviations used: CBB, Coomassie Brilliant Blue; DC, docking complex; EDC, 1-ethyl-3-[3-dimethylaminopropyl]-carbodiimide; HC, heavy chain; IC, intermediate chain; LC, light chain; Robl, roadblock.

Corresponding author. E-mail address: steve{at}king2.uchc.edu.

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