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Originally published as MBC in Press, 10.1091/mbc.E04-05-0411 on September 8, 2004

Vol. 15, Issue 11, 5021-5037, November 2004

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Arf1p Provides an Unexpected Link between COPI Vesicles and mRNA in Saccharomyces cerevisiae{boxd}

Mark Trautwein *, Jörn Dengjel {dagger}, Markus Schirle {dagger}, and Anne Spang * {ddagger}

* Friedrich Miescher Laboratorium, Max Planck Gesellschaft, D-72076 Tübingen, Germany; {dagger} Universität Tübingen, Interfakultäres Institut, D-72076 Tübingen, Germany

Submitted May 18, 2004; Revised August 26, 2004; Accepted August 27, 2004
Monitoring Editor: Howard Riezman

The small GTPase Arf1p is involved in different cellular processes that require its accumulation at specific cellular locations. The recruitment of Arf1p to distinct points of action might be achieved by association of Arf1p with different proteins. To identify new interactors of Arf1p, we performed an affinity chromatography with GTP- or GDP-bound Arf1p proteins. A new interactor of Arf1p-GTP was identified as Pab1p, which binds to the polyA-tail of mRNAs. Pab1p was found to associate with purified COPI-coated vesicles generated from Golgi membranes in vitro. The stability of the Pab1p–Arf1p complex depends on the presence of mRNA. Both symmetrically distributed mRNAs as well as the asymmetrically localized ASH1 mRNA are found in association with Arf1p. Remarkably, Arf1p and Pab1p are both required to restrict ASH1 mRNA to the bud tip. Arf1p and coatomer play an unexpected role in localizing mRNA independent and downstream of the SHE machinery. Hereby acts the SHE machinery in long-range mRNA transport, whereas COPI vesicles could act as short-range and localization vehicles. The endoplasmic reticulum (ER)–Golgi shuttle might be involved in concentrating mRNA at the ER.

Article published online ahead of print. Mol. Biol. Cell 10.1091/mbc.E04–05–0411. Article and publication date are available at www.molbiolcell.org/cgi/doi/10.1091/mbc.E04–05–0411.

{boxd} The online version of this article contains supplementary material accessible through http://www.molbiolcell.org.

{ddagger} Corresponding author. E-mail address: anne.spang{at}tuebingen.mpg.de.




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