![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 15, Issue 12, 5208-5218, December 2004
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892-5431
Submitted July 14, 2004;
Accepted September 15, 2004
Monitoring Editor: Joseph Gall
There are three mammalian SUMO paralogues: SUMO-1 is 
45% identical to SUMO-2 and SUMO-3, which are 96% identical to each other. It is currently unclear whether SUMO-1, -2, and -3 function in ways that are unique, redundant, or antagonistic. To address this question, we examined the dynamics of individual SUMO paralogues by using cell lines that stably express each of the mammalian SUMO proteins fused to the yellow fluorescent protein (YFP). Whereas SUMO-2 and -3 showed very similar distributions throughout the nucleoplasm, SUMO-1 was uniquely distributed to the nuclear envelope and to the nucleolus. Photobleaching experiments revealed that SUMO-1 dynamics was much slower than SUMO-2 and -3 dynamics. Additionally, the mobility of SUMO paralogues differed between subnuclear structures. Finally, the timing and distributions were dissimilar between paralogues as cells exited from mitosis. SUMO-1 was recruited to nuclear membrane as nuclear envelopes reformed in late anaphase, and accumulated rapidly into the nucleus. SUMO-2 and SUMO-3 localized to chromosome earlier and accumulated gradually during telophase. Together, these findings demonstrate that mammalian SUMO-1 shows patterns of utilization that are clearly discrete from the patterns of SUMO-2 and -3 throughout the cell cycle, arguing that it is functionally distinct and specifically regulated in vivo.
Abbreviations used: GFP, green fluorescent protein; YFP, yellow fluorescent protein.
The online version of this article contains supplemental material at MBC Online (http://www.molbiolcell.org).
* Corresponding author. E-mail address: mdasso{at}helix.nih.gov.
This article has been cited by other articles:
|
|
 |
|
  Y. Wang and M. Dasso SUMOylation and deSUMOylation at a glance J. Cell Sci., December 1, 2009; 122(23): 4249 - 4252. [Full Text] [PDF]
|
|
|
|
 |
|
 S. Messner, D. Schuermann, M. Altmeyer, I. Kassner, D. Schmidt, P. Schar, S. Muller, and M. O. Hottiger Sumoylation of poly(ADP-ribose) polymerase 1 inhibits its acetylation and restrains transcriptional coactivator function FASEB J, November 1, 2009; 23(11): 3978 - 3989. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Percherancier, D. Germain-Desprez, F. Galisson, X. H. Mascle, L. Dianoux, P. Estephan, M. K. Chelbi-Alix, and M. Aubry Role of SUMO in RNF4-mediated Promyelocytic Leukemia Protein (PML) Degradation: Sumoylation of pml and phospho-switch control of its sumo binding domain dissected in living cells J. Biol. Chem., June 12, 2009; 284(24): 16595 - 16608. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. R. Klein, M. Haindl, E. A. Nigg, and S. Muller RanBP2 and SENP3 Function in a Mitotic SUMO2/3 Conjugation-Deconjugation Cycle on Borealin Mol. Biol. Cell, January 1, 2009; 20(1): 410 - 418. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Sekiyama, T. Ikegami, T. Yamane, M. Ikeguchi, Y. Uchimura, D. Baba, M. Ariyoshi, H. Tochio, H. Saitoh, and M. Shirakawa Structure of the Small Ubiquitin-like Modifier (SUMO)-interacting Motif of MBD1-containing Chromatin-associated Factor 1 Bound to SUMO-3 J. Biol. Chem., December 19, 2008; 283(51): 35966 - 35975. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Evdokimov, P. Sharma, S. J. Lockett, M. Lualdi, and M. R. Kuehn Loss of SUMO1 in mice affects RanGAP1 localization and formation of PML nuclear bodies, but is not lethal as it can be compensated by SUMO2 or SUMO3 J. Cell Sci., December 15, 2008; 121(24): 4106 - 4113. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Yun, Y. Wang, D. Mukhopadhyay, P. Backlund, N. Kolli, A. Yergey, K. D. Wilkinson, and M. Dasso Nucleolar protein B23/nucleophosmin regulates the vertebrate SUMO pathway through SENP3 and SENP5 proteases J. Cell Biol., November 17, 2008; 183(4): 589 - 595. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. D. Lima and D. Reverter Structure of the Human SENP7 Catalytic Domain and Poly-SUMO Deconjugation Activities for SENP6 and SENP7 J. Biol. Chem., November 14, 2008; 283(46): 32045 - 32055. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Ihara, P. Stein, and R. M. Schultz UBE2I (UBC9), a SUMO-Conjugating Enzyme, Localizes to Nuclear Speckles and Stimulates Transcription in Mouse Oocytes Biol Reprod, November 1, 2008; 79(5): 906 - 913. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Zhu, S. Zhu, C. M. Guzzo, N. A. Ellis, K. S. Sung, C. Y. Choi, and M. J. Matunis Small Ubiquitin-related Modifier (SUMO) Binding Determines Substrate Recognition and Paralog-selective SUMO Modification J. Biol. Chem., October 24, 2008; 283(43): 29405 - 29415. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Lee, Y. Lee, M. J. Lee, E. Park, S. H. Kang, C. H. Chung, K. H. Lee, and K. Kim Dual Modification of BMAL1 by SUMO2/3 and Ubiquitin Promotes Circadian Activation of the CLOCK/BMAL1 Complex Mol. Cell. Biol., October 1, 2008; 28(19): 6056 - 6065. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Gao, C.-C. Ho, E. Reineke, M. Lam, X. Cheng, K. J. Stanya, Y. Liu, S. Chakraborty, H.-M. Shih, and H.-Y. Kao Histone Deacetylase 7 Promotes PML Sumoylation and Is Essential for PML Nuclear Body Formation Mol. Cell. Biol., September 15, 2008; 28(18): 5658 - 5667. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Agostinho, V. Santos, F. Ferreira, R. Costa, J. Cardoso, I. Pinheiro, J. Rino, E. Jaffray, R. T. Hay, and J. Ferreira Conjugation of Human Topoisomerase 2{alpha} with Small Ubiquitin-like Modifiers 2/3 in Response to Topoisomerase Inhibitors: Cell Cycle Stage and Chromosome Domain Specificity Cancer Res., April 1, 2008; 68(7): 2409 - 2418. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. F. Deyrieux, G. Rosas-Acosta, M. A. Ozbun, and V. G. Wilson Sumoylation dynamics during keratinocyte differentiation J. Cell Sci., January 1, 2007; 120(1): 125 - 136. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Li, R. Santockyte, R.-F. Shen, E. Tekle, G. Wang, D. C. H. Yang, and P. B. Chock Expression of SUMO-2/3 Induced Senescence through p53- and pRB-mediated Pathways J. Biol. Chem., November 24, 2006; 281(47): 36221 - 36227. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Mukhopadhyay, F. Ayaydin, N. Kolli, S.-H. Tan, T. Anan, A. Kametaka, Y. Azuma, K. D. Wilkinson, and M. Dasso SUSP1 antagonizes formation of highly SUMO2/3-conjugated species J. Cell Biol., September 25, 2006; 174(7): 939 - 949. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Di Bacco, J. Ouyang, H.-Y. Lee, A. Catic, H. Ploegh, and G. Gill The SUMO-Specific Protease SENP5 Is Required for Cell Division Mol. Cell. Biol., June 15, 2006; 26(12): 4489 - 4498. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-M. Hecker, M. Rabiller, K. Haglund, P. Bayer, and I. Dikic Specification of SUMO1- and SUMO2-interacting Motifs J. Biol. Chem., June 9, 2006; 281(23): 16117 - 16127. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. Baron, C. G. Tepper, S. Y. Liu, R. R. Davis, N. J. Wang, N. C. Schanen, and J. P. Gregg Genomic and functional profiling of duplicated chromosome 15 cell lines reveal regulatory alterations in UBE3A-associated ubiquitin-proteasome pathway processes Hum. Mol. Genet., March 15, 2006; 15(6): 853 - 869. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Yurchenko, Z. Xue, and M. J. Sadofsky SUMO Modification of Human XRCC4 Regulates Its Localization and Function in DNA Double-Strand Break Repair Mol. Cell. Biol., March 1, 2006; 26(5): 1786 - 1794. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Hietakangas, J. Anckar, H. A. Blomster, M. Fujimoto, J. J. Palvimo, A. Nakai, and L. Sistonen PDSM, a motif for phosphorylation-dependent SUMO modification PNAS, January 3, 2006; 103(1): 45 - 50. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Song, Z. Zhang, W. Hu, and Y. Chen Small Ubiquitin-like Modifier (SUMO) Recognition of a SUMO Binding Motif: A REVERSAL OF THE BOUND ORIENTATION J. Biol. Chem., December 2, 2005; 280(48): 40122 - 40129. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M.P. Desterro, L. P. Keegan, E. Jaffray, R. T. Hay, M. A. O'Connell, and M. Carmo-Fonseca SUMO-1 Modification Alters ADAR1 Editing Activity Mol. Biol. Cell, November 1, 2005; 16(11): 5115 - 5126. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. M. Veltman, L. A. Vreede, J. Cheng, L. H.J. Looijenga, B. Janssen, E. F.P.M. Schoenmakers, E. T.H. Yeh, and A. G. van Kessel Fusion of the SUMO/Sentrin-specific protease 1 gene SENP1 and the embryonic polarity-related mesoderm development gene MESDC2 in a patient with an infantile teratoma and a constitutional t(12;15)(q13;q25) Hum. Mol. Genet., July 15, 2005; 14(14): 1955 - 1963. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Gregoire and X.-J. Yang Association with Class IIa Histone Deacetylases Upregulates the Sumoylation of MEF2 Transcription Factors Mol. Cell. Biol., March 15, 2005; 25(6): 2273 - 2287. [Abstract] [Full Text] [PDF]
|
|
