![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 15, Issue 12, 5231-5241, December 2004
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
* Laboratoire de Biologie Moléculaire de la Cellule, ENS/CNRS 5161, IFR 128 Biosciences Lyon-Gerland, 69007 Lyon, France;
Cell and Developmental Biology Department, University of California, La Jolla, CA 92093-0347; and
European Synchrotron Radiation Facility (ESRF), X-Ray Microscopy, BP 220 38043 Grenoble, France
Submitted June 25, 2004;
Accepted September 1, 2004
Monitoring Editor: Thomas Pollard
The actin cytoskeleton is essential for osteoclasts main function, bone resorption. Two different organizations of actin have been described in osteoclasts, the podosomes belt corresponding to numerous F-actin columns arranged at the cell periphery, and the sealing zone defined as a unique large band of actin. To compare the role of these two different actin organizations, we imaged osteoclasts on various substrata: glass, dentin, and apatite. Using primary osteoclasts expressing GFP-actin, we found that podosome belts and sealing zones, both very dynamic actin structures, were present in mature osteoclasts; podosome belts were observed only in spread osteoclasts adhering onto glass, whereas sealing zone were seen in apico-basal polarized osteoclasts adherent on mineralized matrix. Dynamic observations of several resorption cycles of osteoclasts seeded on apatite revealed that 1) podosomes do not fuse together to form the sealing zone; 2) osteoclasts alternate successive stationary polarized resorption phases with a sealing zone and migration, nonresorption phases without any specific actin structure; and 3) apatite itself promotes sealing zone formation though c-src and Rho signaling. Finally, our work suggests that apatite-mediated sealing zone formation is dependent on both c-src and Rho whereas apico-basal polarization requires only Rho.
The online version of this article contains supplemental material at MBC Online (http://www.molbiolcell.org).
These authors contributed equally to this work.
|| Corresponding author. E-mail address: pjurdic{at}ens-lyon.fr.
This article has been cited by other articles:
|
|
 |
|
  T. Heckel, C. Czupalla, A. I. Expirto Santo, M. Anitei, M. Arantzazu Sanchez-Fernandez, K. Mosch, E. Krause, and B. Hoflack Src-dependent repression of ARF6 is required to maintain podosome-rich sealing zones in bone-digesting osteoclasts PNAS, February 3, 2009; 106(5): 1451 - 1456. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. R. Wilson, C. Peters, P. Saftig, and D. Bromme Cathepsin K Activity-dependent Regulation of Osteoclast Actin Ring Formation and Bone Resorption J. Biol. Chem., January 23, 2009; 284(4): 2584 - 2592. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Chabadel, I. Banon-Rodriguez, D. Cluet, B. B. Rudkin, B. Wehrle-Haller, E. Genot, P. Jurdic, I. M. Anton, and F. Saltel CD44 and beta3 Integrin Organize Two Functionally Distinct Actin-based Domains in Osteoclasts Mol. Biol. Cell, December 1, 2007; 18(12): 4899 - 4910. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Chellaiah, D. Kuppuswamy, L. Lasky, and S. Linder Phosphorylation of a Wiscott-Aldrich Syndrome Protein-associated Signal Complex Is Critical in Osteoclast Bone Resorption J. Biol. Chem., March 30, 2007; 282(13): 10104 - 10116. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Valcourt, B. Merle, E. Gineyts, S. Viguet-Carrin, P. D. Delmas, and P. Garnero Non-enzymatic Glycation of Bone Collagen Modifies Osteoclastic Activity and Differentiation J. Biol. Chem., February 23, 2007; 282(8): 5691 - 5703. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Edwards, C. Cohen, W. Xu, and P. H. Schlesinger c-Src Control of Chloride Channel Support for Osteoclast HCl Transport and Bone Resorption J. Biol. Chem., September 22, 2006; 281(38): 28011 - 28022. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Tehrani, R. Faccio, I. Chandrasekar, F. P. Ross, and J. A. Cooper Cortactin Has an Essential and Specific Role in Osteoclast Actin Assembly Mol. Biol. Cell, July 1, 2006; 17(7): 2882 - 2895. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Collin, P. Tracqui, A. Stephanou, Y. Usson, J. Clement-Lacroix, and E. Planus Spatiotemporal dynamics of actin-rich adhesion microdomains: influence of substrate flexibility. J. Cell Sci., May 1, 2006; 119(Pt 9): 1914 - 1925. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Akisaka, H. Yoshida, and R. Suzuki The ruffled border and attachment regions of the apposing membrane of resorbing osteoclasts as visualized from the cytoplasmic face of the membrane J. Electron Microsc. (Tokyo), April 1, 2006; 55(2): 53 - 61. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Fuller, B. Kirstein, and T. J. Chambers Murine Osteoclast Formation and Function: Differential Regulation by Humoral Agents Endocrinology, April 1, 2006; 147(4): 1979 - 1985. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Rucci, C. DiGiacinto, L. Orru, D. Millimaggi, R. Baron, and A. Teti A novel protein kinase C {alpha}-dependent signal to ERK1/2 activated by {alpha}V{beta}3 integrin in osteoclasts and in Chinese hamster ovary (CHO) cells J. Cell Sci., August 1, 2005; 118(15): 3263 - 3275. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Destaing, F. Saltel, B. Gilquin, A. Chabadel, S. Khochbin, S. Ory, and P. Jurdic A novel Rho-mDia2-HDAC6 pathway controls podosome patterning through microtubule acetylation in osteoclasts J. Cell Sci., July 1, 2005; 118(13): 2901 - 2911. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. J. Pavlos, J. Xu, D. Riedel, J. S. G. Yeoh, S. L. Teitelbaum, J. M. Papadimitriou, R. Jahn, F. P. Ross, and M. H. Zheng Rab3D Regulates a Novel Vesicular Trafficking Pathway That Is Required for Osteoclastic Bone Resorption Mol. Cell. Biol., June 15, 2005; 25(12): 5253 - 5269. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Linder and P. Kopp Podosomes at a glance J. Cell Sci., May 15, 2005; 118(10): 2079 - 2082. [Full Text] [PDF]
|
|
