![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 15, Issue 12, 5431-5442, December 2004
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
* Department of Cell Biology, Emory University School of Medicine, Atlanta, GA 30322;
Department of Genetics/Cell and Developmental Biology, University of Minnesota, Minneapolis, MN 55455; and
Department of Biology, Marquette University, Milwaukee, WI 53201
Submitted August 12, 2004;
Revised September 20, 2004;
Accepted September 22, 2004
Monitoring Editor: Thomas Pollard
Increased phosphorylation of dynein IC IC138 correlates with decreases in flagellar microtubule sliding and phototaxis defects. To test the hypothesis that regulation of IC138 phosphorylation controls flagellar bending, we cloned the IC138 gene. IC138 encodes a novel protein with a calculated mass of 111 kDa and is predicted to form seven WD-repeats at the C terminus. IC138 maps near the BOP5 locus, and bop5-1 contains a point mutation resulting in a truncated IC138 lacking the C terminus, including the seventh WD-repeat. bop5-1 cells display wild-type flagellar beat frequency but swim slower than wild-type cells, suggesting that bop5-1 is altered in its ability to control flagellar waveform. Swimming speed is rescued in bop5-1 transformants containing the wild-type IC138, confirming that BOP5 encodes IC138. With the exception of the roadblock-related light chain, LC7b, all the other known components of the I1 complex, including the truncated IC138, are assembled in bop5-1 axonemes. Thus, the bop5-1 motility phenotype reveals a role for IC138 and LC7b in the control of flagellar bending. IC138 is hyperphosphorylated in paralyzed flagellar mutants lacking radial spoke and central pair components, further indicating a role for the radial spokes and central pair apparatus in control of IC138 phosphorylation and regulation of flagellar waveform.
Corresponding author. E-mail address: win{at}cellbio.emory.edu.
This article has been cited by other articles:
|
|
 |
|
  C. B. Lindemann and K. A. Lesich Flagellar and ciliary beating: the proven and the possible J. Cell Sci., February 15, 2010; 123(4): 519 - 528. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Yang, C. Yang, M. Wirschell, and S. Davis Novel LC8 Mutations Have Disparate Effects on the Assembly and Stability of Flagellar Complexes J. Biol. Chem., November 6, 2009; 284(45): 31412 - 31421. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Gokhale, M. Wirschell, and W. S. Sale Regulation of dynein-driven microtubule sliding by the axonemal protein kinase CK1 in Chlamydomonas flagella J. Cell Biol., September 21, 2009; 186(6): 817 - 824. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Boesger, V. Wagner, W. Weisheit, and M. Mittag Analysis of Flagellar Phosphoproteins from Chlamydomonas reinhardtii Eukaryot. Cell, July 1, 2009; 8(7): 922 - 932. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Bower, K. VanderWaal, E. O'Toole, L. Fox, C. Perrone, J. Mueller, M. Wirschell, R. Kamiya, W. S. Sale, and M. E. Porter IC138 Defines a Subdomain at the Base of the I1 Dynein That Regulates Microtubule Sliding and Flagellar Motility Mol. Biol. Cell, July 1, 2009; 20(13): 3055 - 3063. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. H. Bui, H. Sakakibara, T. Movassagh, K. Oiwa, and T. Ishikawa Molecular architecture of inner dynein arms in situ in Chlamydomonas reinhardtii flagella J. Cell Biol., December 1, 2008; 183(5): 923 - 932. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. Tanner, P. Rompolas, R. S. Patel-King, O. Gorbatyuk, K.-i. Wakabayashi, G. J. Pazour, and S. M. King Three Members of the LC8/DYNLL Family Are Required for Outer Arm Dynein Motor Function Mol. Biol. Cell, September 1, 2008; 19(9): 3724 - 3734. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. E. Dymek and E. F. Smith A conserved CaM- and radial spoke associated complex mediates regulation of flagellar dynein activity J. Cell Biol., November 5, 2007; 179(3): 515 - 526. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Hou, H. Qin, J. A. Follit, G. J. Pazour, J. L. Rosenbaum, and G. B. Witman Functional analysis of an individual IFT protein: IFT46 is required for transport of outer dynein arms into flagella J. Cell Biol., February 26, 2007; 176(5): 653 - 665. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. W.-H. Lo, H.-M. Kan, and K. K. Pfister Identification of a Novel Region of the Cytoplasmic Dynein Intermediate Chain Important for Dimerization in the Absence of the Light Chains J. Biol. Chem., April 7, 2006; 281(14): 9552 - 9559. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Wagner, G. Gessner, I. Heiland, M. Kaminski, S. Hawat, K. Scheffler, and M. Mittag Analysis of the Phosphoproteome of Chlamydomonas reinhardtii Provides New Insights into Various Cellular Pathways Eukaryot. Cell, March 1, 2006; 5(3): 457 - 468. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Yang and P. Yang The Flagellar Motility of Chlamydomonas pf25 Mutant Lacking an AKAP-binding Protein Is Overtly Sensitive to Medium Conditions Mol. Biol. Cell, January 1, 2006; 17(1): 227 - 238. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. M. DiBella, O. Gorbatyuk, M. Sakato, K.-i. Wakabayashi, R. S. Patel-King, G. J. Pazour, G. B. Witman, and S. M. King Differential Light Chain Assembly Influences Outer Arm Dynein Motor Function Mol. Biol. Cell, December 1, 2005; 16(12): 5661 - 5674. [Abstract] [Full Text] [PDF]
|
|
