QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

Vol. 15, Issue 2, 600-610, February 2004

This Article Full Text Full Text (PDF) All Versions of this Article: E03-06-0374v1 15/2/600    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Patterson, K. Articles by Fischer, J. A. Search for Related Content PubMed PubMed Citation Articles by Patterson, K. Articles by Fischer, J. A.

The Functions of Klarsicht and Nuclear Lamin in Developmentally Regulated Nuclear Migrations of Photoreceptor Cells in the Drosophila Eye

Kristin Patterson, Ari B. Molofsky ^*, Christina Robinson, Shelley Acosta, Courtney Cater, and Janice A. Fischer 

Section of Molecular Cell and Developmental Biology, Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, Texas 78712

Submitted June 8, 2003; Revised October 17, 2003; Accepted October 27, 2003
Monitoring Editor: Paul Matsudaira

Photoreceptor nuclei in the Drosophila eye undergo developmentally regulated migrations. Nuclear migration is known to require the perinuclear protein Klarsicht, but the function of Klarsicht has been obscure. Here, we show that Klarsicht is required for connecting the microtubule organizing center (MTOC) to the nucleus. In addition, in a genetic screen for klarsicht-interacting genes, we identified Lam Dm₀, which encodes nuclear lamin. We find that, like Klarsicht, lamin is required for photoreceptor nuclear migration and for nuclear attachment to the MTOC. Moreover, perinuclear localization of Klarsicht requires lamin. We propose that nuclear migration requires linkage of the MTOC to the nucleus through an interaction between microtubules, Klarsicht, and lamin. The Klarsicht/lamin interaction provides a framework for understanding the mechanistic basis of human laminopathies.

^* Present address: Department of Microbiology and Immunology, The University of Michigan Medical School, 6734 Medical Sciences Building II, Ann Arbor, Michigan 48109.

Corresponding author. E-mail address: jaf{at}mail.utexas.edu.

This article has been cited by other articles:

				K. Wilhelmsen, M. Ketema, H. Truong, and A. Sonnenberg KASH-domain proteins in nuclear migration, anchorage and other processes J. Cell Sci., December 15, 2006; 119(24): 5021 - 5029. [Abstract] [Full Text] [PDF]

				J. L. V. Broers, F. C. S. Ramaekers, G. Bonne, R. B. Yaou, and C. J. Hutchison Nuclear lamins: laminopathies and their role in premature ageing. Physiol Rev, July 1, 2006; 86(3): 967 - 1008. [Abstract] [Full Text] [PDF]

				M. D. McGee, R. Rillo, A. S. Anderson, and D. A. Starr UNC-83 Is a KASH Protein Required for Nuclear Migration and Is Recruited to the Outer Nuclear Membrane by a Physical Interaction with the SUN Protein UNC-84 Mol. Biol. Cell, April 1, 2006; 17(4): 1790 - 1801. [Abstract] [Full Text] [PDF]

				A. Pramatarova, P. G. Ochalski, C.-H. Lee, and B. W. Howell Mouse Disabled 1 Regulates the Nuclear Position of Neurons in a Drosophila Eye Model Mol. Cell. Biol., February 15, 2006; 26(4): 1510 - 1517. [Abstract] [Full Text] [PDF]

				X. Bao, W. Zhang, R. Krencik, H. Deng, Y. Wang, J. Girton, J. Johansen, and K. M. Johansen The JIL-1 kinase interacts with lamin Dm0 and regulates nuclear lamina morphology of Drosophila nurse cells J. Cell Sci., November 1, 2005; 118(21): 5079 - 5087. [Abstract] [Full Text] [PDF]

				S. R. Schulze, B. Curio-Penny, Y. Li, R. A. Imani, L. Rydberg, P. K. Geyer, and L. L. Wallrath Molecular Genetic Analysis of the Nested Drosophila melanogaster Lamin C Gene Genetics, September 1, 2005; 171(1): 185 - 196. [Abstract] [Full Text] [PDF]

				V.C. Padmakumar, T. Libotte, W. Lu, H. Zaim, S. Abraham, A. A. Noegel, J. Gotzmann, R. Foisner, and I. Karakesisoglou The inner nuclear membrane protein Sun1 mediates the anchorage of Nesprin-2 to the nuclear envelope J. Cell Sci., August 1, 2005; 118(15): 3419 - 3430. [Abstract] [Full Text] [PDF]

				R. M. Grady, D. A. Starr, G. L. Ackerman, J. R. Sanes, and M. Han From the Cover: Syne proteins anchor muscle nuclei at the neuromuscular junction PNAS, March 22, 2005; 102(12): 4359 - 4364. [Abstract] [Full Text] [PDF]

				Y. Guo, S. Jangi, and M. A. Welte Organelle-specific Control of Intracellular Transport: Distinctly Targeted Isoforms of the Regulator Klar Mol. Biol. Cell, March 1, 2005; 16(3): 1406 - 1416. [Abstract] [Full Text] [PDF]

				Q. Zhang, C. D. Ragnauth, J. N. Skepper, N. F. Worth, D. T. Warren, R. G. Roberts, P. L. Weissberg, J. A. Ellis, and C. M. Shanahan Nesprin-2 is a multi-isomeric protein that binds lamin and emerin at the nuclear envelope and forms a subcellular network in skeletal muscle J. Cell Sci., February 15, 2005; 118(4): 673 - 687. [Abstract] [Full Text] [PDF]

				J. L.V. Broers, E. A.G. Peeters, H. J.H. Kuijpers, J. Endert, C. V.C. Bouten, C. W.J. Oomens, F. P.T. Baaijens, and F. C.S. Ramaekers Decreased mechanical stiffness in LMNA-/- cells is caused by defective nucleo-cytoskeletal integrity: implications for the development of laminopathies Hum. Mol. Genet., November 1, 2004; 13(21): 2567 - 2580. [Abstract] [Full Text] [PDF]

				J. A. Fischer, S. Acosta, A. Kenny, C. Cater, C. Robinson, and J. Hook Drosophila Klarsicht Has Distinct Subcellular Localization Domains for Nuclear Envelope and Microtubule Localization in the Eye Genetics, November 1, 2004; 168(3): 1385 - 1393. [Abstract] [Full Text] [PDF]

				J. L. Whited, A. Cassell, M. Brouillette, and P. A. Garrity Dynactin is required to maintain nuclear position within postmitotic Drosophila photoreceptor neurons Development, October 1, 2004; 131(19): 4677 - 4686. [Abstract] [Full Text] [PDF]